YNL219C / ALG9

Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL219C / ALG9

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-12.60|P-value:9.07E-34 ID:SGTC_122|Compound:0236-0016|FD-Score:3.67|P-value:2.37E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.32|P-value:1.78E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:4.02|P-value:6.50E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.86|P-value:1.19E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.38|P-value:6.45E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.29|P-value:8.12E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:6.77|P-value:5.79E-11 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.32|P-value:7.32E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.96|P-value:7.58E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.80|P-value:1.37E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.51|P-value:4.15E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.79|P-value:2.57E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:4.00|P-value:7.00E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:3.29|P-value:8.91E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.24|P-value:2.76E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:4.21|P-value:3.15E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:3.54|P-value:3.75E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.33|P-value:7.17E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.28|P-value:9.02E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.09|P-value:4.97E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.89|P-value:1.60E-6 ID:SGTC_1716|Compound:st033144|FD-Score:-4.33|P-value:1.73E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.41|P-value:5.46E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.13|P-value:4.22E-5 ID:SGTC_1857|Compound:st056007|FD-Score:3.57|P-value:3.43E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.21|P-value:2.87E-5 ID:SGTC_2009|Compound:4012297|FD-Score:5.30|P-value:2.22E-7 ID:SGTC_2021|Compound:4019055|FD-Score:4.40|P-value:1.39E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.83|P-value:1.25E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-3.46|P-value:4.74E-4 ID:SGTC_2077|Compound:5212015|FD-Score:-3.52|P-value:3.80E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.72|P-value:3.54E-6 ID:SGTC_2453|Compound:5731132|FD-Score:-3.87|P-value:1.06E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-4.12|P-value:4.13E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.71|P-value:2.07E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.68|P-value:2.30E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.34|P-value:7.52E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.29|P-value:8.87E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.85|P-value:1.94E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.52|P-value:4.02E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.70|P-value:2.14E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.13|P-value:2.69E-9 ID:SGTC_2791|Compound:5663188|FD-Score:-3.48|P-value:4.37E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.50|P-value:4.44E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.23|P-value:3.24E-7 ID:SGTC_2967|Compound:9089538|FD-Score:5.29|P-value:2.36E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.48|P-value:4.67E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.87|P-value:1.17E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.03|P-value:6.28E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.42|P-value:5.73E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-4.26|P-value:2.33E-5 ID:SGTC_3292|Compound:9120825|FD-Score:-3.30|P-value:7.82E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-12.60|P-value:9.07E-34 ID:SGTC_122|Compound:0236-0016|FD-Score:3.67|P-value:2.37E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.32|P-value:1.78E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:4.02|P-value:6.50E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.86|P-value:1.19E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.38|P-value:6.45E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.29|P-value:8.12E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:6.77|P-value:5.79E-11 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.32|P-value:7.32E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.96|P-value:7.58E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.80|P-value:1.37E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.51|P-value:4.15E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.79|P-value:2.57E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:4.00|P-value:7.00E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:3.29|P-value:8.91E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.24|P-value:2.76E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:4.21|P-value:3.15E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:3.54|P-value:3.75E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.33|P-value:7.17E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.28|P-value:9.02E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.09|P-value:4.97E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.89|P-value:1.60E-6 ID:SGTC_1716|Compound:st033144|FD-Score:-4.33|P-value:1.73E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.41|P-value:5.46E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.13|P-value:4.22E-5 ID:SGTC_1857|Compound:st056007|FD-Score:3.57|P-value:3.43E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.21|P-value:2.87E-5 ID:SGTC_2009|Compound:4012297|FD-Score:5.30|P-value:2.22E-7 ID:SGTC_2021|Compound:4019055|FD-Score:4.40|P-value:1.39E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.83|P-value:1.25E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-3.46|P-value:4.74E-4 ID:SGTC_2077|Compound:5212015|FD-Score:-3.52|P-value:3.80E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.72|P-value:3.54E-6 ID:SGTC_2453|Compound:5731132|FD-Score:-3.87|P-value:1.06E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-4.12|P-value:4.13E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.71|P-value:2.07E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.68|P-value:2.30E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.34|P-value:7.52E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.29|P-value:8.87E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.85|P-value:1.94E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.52|P-value:4.02E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.70|P-value:2.14E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.13|P-value:2.69E-9 ID:SGTC_2791|Compound:5663188|FD-Score:-3.48|P-value:4.37E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.50|P-value:4.44E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.23|P-value:3.24E-7 ID:SGTC_2967|Compound:9089538|FD-Score:5.29|P-value:2.36E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.48|P-value:4.67E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.87|P-value:1.17E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.03|P-value:6.28E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.42|P-value:5.73E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-4.26|P-value:2.33E-5 ID:SGTC_3292|Compound:9120825|FD-Score:-3.30|P-value:7.82E-4

Top fitness defect scores for YNL219C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_312 4092-0653 6.77 6.8 µM 5.79E-11 ergosterol depletion effects on membrane 26 6.59E-12
2 SGTC_2688 kanamycin b 6.13 96.7 µM 2.69E-9 calcium & mitochondrial duress 151 4.46E-10
3 SGTC_2009 4012297 5.30 12.1 µM 2.22E-7 cell wall signaling 34 5.67E-8
4 SGTC_2967 9089538 5.29 49.9 µM 2.36E-7 9 6.08E-8
5 SGTC_2896 9052656 5.23 71.4 µM 3.24E-7 endomembrane recycling 52 8.60E-8
6 SGTC_1713 st033146 4.89 12.8 µM 1.60E-6 11 4.95E-7
7 SGTC_2552 juarezic acid 4.85 87.0 µM 1.94E-6 TSC3-RPN4 14 6.15E-7
8 SGTC_568 1193-0097 4.79 7.5 µM 2.57E-6 ERAD & cell cycle 97 8.33E-7
9 SGTC_2413 inermin 4.72 26.1 µM 3.54E-6 34 1.19E-6
10 SGTC_2021 4019055 4.40 173.0 µM 1.39E-5 endomembrane recycling 97 5.31E-6
11 SGTC_800 3448-7362 4.24 155.0 µM 2.76E-5 translation 139 1.13E-5
12 SGTC_860 0443-0016 4.21 25.1 µM 3.15E-5 25 1.31E-5
13 SGTC_1789 tunicamycin 4.13 25.0 nM 4.22E-5 unfolded protein response 40 1.80E-5
14 SGTC_1597 7-benzyloxygramine 4.09 47.5 µM 4.97E-5 calcium & mitochondrial duress 113 2.15E-5
15 SGTC_3108 9121785 4.03 49.5 µM 6.28E-5 12 2.78E-5
16 SGTC_143 4032-1194 4.02 15.7 µM 6.50E-5 62 2.89E-5
17 SGTC_637 1181-0519 4.00 7.3 µM 7.00E-5 fatty acid desaturase (OLE1) 100 3.13E-5
18 SGTC_3037 9091026 3.87 49.5 µM 1.17E-4 NEO1-PIK1 128 5.48E-5
19 SGTC_255 tunicamycin 3.86 200.5 nM 1.19E-4 unfolded protein response 77 5.58E-5
20 SGTC_776 4476-4378 3.74 112.0 µM 1.84E-4 ERG2 24 9.06E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.248 2.27E-48 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.209 1.90E-34 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.201 5.28E-32 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.201 6.71E-32 YDR156W RPA14 hom RNA polymerase I subunit A14
0.199 3.97E-31 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.189 2.20E-28 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.189 2.28E-28 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.182 1.55E-26 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.181 2.94E-26 YML019W OST6 hom Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.180 7.14E-26 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.179 1.12E-25 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.174 3.92E-24 YFR040W SAP155 hom Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
0.171 1.61E-23 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.166 4.00E-22 YKL074C MUD2 hom Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.165 8.85E-22 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane