YNL227C / JJJ1

Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL227C / JJJ1

Click on Significant Values for Screen Details ID:SGTC_62|Compound:0180-0423|FD-Score:3.71|P-value:7.94E-5 ID:SGTC_97|Compound:0535-0701|FD-Score:3.66|P-value:9.48E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:7.15|P-value:5.16E-13 ID:SGTC_200|Compound:k038-0079|FD-Score:-4.10|P-value:6.83E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.17|P-value:5.61E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.31|P-value:3.49E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.30|P-value:3.61E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.92|P-value:3.62E-7 ID:SGTC_645|Compound:0845-0943|FD-Score:3.94|P-value:3.12E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:3.04|P-value:8.83E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:3.33|P-value:3.22E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:4.23|P-value:9.22E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:4.29|P-value:6.82E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:4.82|P-value:5.73E-7 ID:SGTC_998|Compound:3996-0085|FD-Score:4.60|P-value:1.65E-6 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.80|P-value:2.13E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.05|P-value:8.56E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:4.18|P-value:1.12E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:-4.28|P-value:3.26E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.26|P-value:4.10E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.65|P-value:3.66E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.57|P-value:4.74E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.94|P-value:3.30E-7 ID:SGTC_1547|Compound:harmine|FD-Score:-3.60|P-value:4.37E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.98|P-value:2.67E-5 ID:SGTC_1602|Compound:st001878|FD-Score:-3.73|P-value:2.75E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.67|P-value:9.28E-5 ID:SGTC_1827|Compound:berberine|FD-Score:3.39|P-value:2.58E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-3.43|P-value:7.78E-4 ID:SGTC_1881|Compound:5652484|FD-Score:-4.75|P-value:4.39E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-3.56|P-value:5.00E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.23|P-value:4.65E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.78|P-value:5.90E-5 ID:SGTC_2511|Compound:berberine|FD-Score:3.36|P-value:2.96E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.39|P-value:2.03E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.46|P-value:2.00E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.84|P-value:3.05E-15 ID:SGTC_2696|Compound:st077691|FD-Score:-3.35|P-value:9.92E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.30|P-value:3.57E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.73|P-value:2.72E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.71|P-value:7.94E-5 ID:SGTC_97|Compound:0535-0701|FD-Score:3.66|P-value:9.48E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:7.15|P-value:5.16E-13 ID:SGTC_200|Compound:k038-0079|FD-Score:-4.10|P-value:6.83E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.17|P-value:5.61E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.31|P-value:3.49E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.30|P-value:3.61E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.92|P-value:3.62E-7 ID:SGTC_645|Compound:0845-0943|FD-Score:3.94|P-value:3.12E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:3.04|P-value:8.83E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:3.33|P-value:3.22E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:4.23|P-value:9.22E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:4.29|P-value:6.82E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:4.82|P-value:5.73E-7 ID:SGTC_998|Compound:3996-0085|FD-Score:4.60|P-value:1.65E-6 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.80|P-value:2.13E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.05|P-value:8.56E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:4.18|P-value:1.12E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:-4.28|P-value:3.26E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.26|P-value:4.10E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.65|P-value:3.66E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.57|P-value:4.74E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.94|P-value:3.30E-7 ID:SGTC_1547|Compound:harmine|FD-Score:-3.60|P-value:4.37E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.98|P-value:2.67E-5 ID:SGTC_1602|Compound:st001878|FD-Score:-3.73|P-value:2.75E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.67|P-value:9.28E-5 ID:SGTC_1827|Compound:berberine|FD-Score:3.39|P-value:2.58E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-3.43|P-value:7.78E-4 ID:SGTC_1881|Compound:5652484|FD-Score:-4.75|P-value:4.39E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-3.56|P-value:5.00E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.23|P-value:4.65E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.78|P-value:5.90E-5 ID:SGTC_2511|Compound:berberine|FD-Score:3.36|P-value:2.96E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.39|P-value:2.03E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.46|P-value:2.00E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.84|P-value:3.05E-15 ID:SGTC_2696|Compound:st077691|FD-Score:-3.35|P-value:9.92E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.30|P-value:3.57E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.73|P-value:2.72E-4

Top fitness defect scores for YNL227C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 7.84 96.7 µM 3.05E-15 calcium & mitochondrial duress 70 2.20E-15
2 SGTC_118 0083-0097 7.15 135.3 µM 5.16E-13 translation 7 4.37E-13
3 SGTC_1521 st012942 4.94 49.4 µM 3.30E-7 12 4.00E-7
4 SGTC_625 k213-0058 4.92 121.0 µM 3.62E-7 translation 59 4.40E-7
5 SGTC_911 3456-4043 4.82 236.0 µM 5.73E-7 mitochondrial processes 15 7.02E-7
6 SGTC_998 3996-0085 4.60 177.0 µM 1.65E-6 9 2.06E-6
7 SGTC_832 4083-0019 4.29 164.0 µM 6.82E-6 translation 72 8.74E-6
8 SGTC_730 1436-0052 4.23 41.2 µM 9.22E-6 mitochondrial processes 9 1.19E-5
9 SGTC_1052 3253-3998 4.18 198.0 µM 1.12E-5 14 1.45E-5
10 SGTC_1564 D-Arginine 3.98 115.0 µM 2.67E-5 26 3.49E-5
11 SGTC_645 0845-0943 3.94 5.4 µM 3.12E-5 60S ribosome export 10 4.09E-5
12 SGTC_2402 5553605 3.78 200.0 µM 5.90E-5 34 7.78E-5
13 SGTC_62 0180-0423 3.71 3.2 µM 7.94E-5 60S ribosome export 25 1.05E-4
14 SGTC_1682 st012968 3.67 530.0 nM 9.28E-5 23 1.23E-4
15 SGTC_97 0535-0701 3.66 19.1 µM 9.48E-5 60S ribosome export 20 1.26E-4
16 SGTC_2687 chenodiol 3.46 100.0 µM 2.00E-4 39 2.68E-4
17 SGTC_1827 berberine 3.39 7.2 µM 2.58E-4 60S ribosome export 34 3.45E-4
18 SGTC_2511 berberine 3.36 10.5 µM 2.96E-4 60S ribosome export 38 3.96E-4
19 SGTC_728 3030-5151 3.33 22.5 µM 3.22E-4 30 4.32E-4
20 SGTC_538 2922-0838 3.31 53.3 µM 3.49E-4 Golgi 57 4.69E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.213 7.68E-36 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.212 2.15E-35 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.197 1.22E-30 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.191 8.52E-29 YDL082W RPL13A hom Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
0.185 2.89E-27 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.173 4.99E-24 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.164 1.18E-21 YMR230W RPS10B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication
0.152 7.64E-19 YDR083W RRP8 hom Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
0.148 7.19E-18 YMR193C-A_d YMR193C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.148 8.07E-18 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.145 2.98E-17 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.141 2.48E-16 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.139 5.02E-16 YIR026C YVH1 hom Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
0.138 9.41E-16 YBR048W RPS11B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
0.138 1.13E-15 YLR264C-A_p YLR264C-A_p hom Putative protein of unknown function