YNL258C / DSL1

Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL258C / DSL1

Click on Significant Values for Screen Details ID:SGTC_130|Compound:0136-0219|FD-Score:3.20|P-value:9.08E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.57|P-value:2.36E-8 ID:SGTC_179|Compound:1273-0063|FD-Score:3.48|P-value:3.45E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.23|P-value:8.22E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.26|P-value:7.45E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.23|P-value:7.54E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.25|P-value:7.15E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:-3.22|P-value:7.78E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.22|P-value:8.52E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.32|P-value:5.96E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.72|P-value:1.44E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.88|P-value:7.72E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.65|P-value:1.72E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:4.18|P-value:2.27E-5 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.19|P-value:9.35E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.01|P-value:4.59E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.33|P-value:5.78E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:5.88|P-value:4.62E-9 ID:SGTC_1428|Compound:4049-0204|FD-Score:-3.78|P-value:1.05E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.19|P-value:2.17E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.28|P-value:6.89E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:5.07|P-value:3.65E-7 ID:SGTC_1775|Compound:st081588|FD-Score:3.35|P-value:5.51E-4 ID:SGTC_1783|Compound:st039459|FD-Score:4.09|P-value:3.29E-5 ID:SGTC_1862|Compound:st057513|FD-Score:3.35|P-value:5.39E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.73|P-value:1.37E-4 ID:SGTC_1887|Compound:st057648|FD-Score:-4.92|P-value:6.93E-7 ID:SGTC_1895|Compound:st058434|FD-Score:-3.84|P-value:8.40E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.44|P-value:3.92E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-4.36|P-value:9.59E-6 ID:SGTC_1904|Compound:st060225|FD-Score:3.82|P-value:9.49E-5 ID:SGTC_1967|Compound:st070177|FD-Score:-4.22|P-value:1.78E-5 ID:SGTC_1978|Compound:st071761|FD-Score:3.24|P-value:7.87E-4 ID:SGTC_2084|Compound:5306078|FD-Score:3.85|P-value:8.48E-5 ID:SGTC_2094|Compound:5332512|FD-Score:4.45|P-value:6.85E-6 ID:SGTC_2125|Compound:5246583|FD-Score:-3.17|P-value:9.37E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-4.71|P-value:1.92E-6 ID:SGTC_2310|Compound:7668677|FD-Score:4.10|P-value:3.13E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-3.67|P-value:1.62E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.55|P-value:2.62E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.61|P-value:1.98E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.57|P-value:4.12E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.41|P-value:8.42E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.66|P-value:1.80E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.55|P-value:4.48E-6 ID:SGTC_2966|Compound:9084041|FD-Score:-3.66|P-value:1.63E-4 ID:SGTC_3029|Compound:9088683|FD-Score:3.44|P-value:3.94E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.32|P-value:5.59E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.50|P-value:3.22E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.26|P-value:7.47E-4 ID:SGTC_3166|Compound:9102297|FD-Score:4.28|P-value:1.47E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.56|P-value:2.43E-4 ID:SGTC_3345|Compound:9148410|FD-Score:3.31|P-value:6.30E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:3.20|P-value:9.08E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.57|P-value:2.36E-8 ID:SGTC_179|Compound:1273-0063|FD-Score:3.48|P-value:3.45E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.23|P-value:8.22E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.26|P-value:7.45E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.23|P-value:7.54E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.25|P-value:7.15E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:-3.22|P-value:7.78E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.22|P-value:8.52E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.32|P-value:5.96E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.72|P-value:1.44E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.88|P-value:7.72E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.65|P-value:1.72E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:4.18|P-value:2.27E-5 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.19|P-value:9.35E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.01|P-value:4.59E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.33|P-value:5.78E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:5.88|P-value:4.62E-9 ID:SGTC_1428|Compound:4049-0204|FD-Score:-3.78|P-value:1.05E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.19|P-value:2.17E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.28|P-value:6.89E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:5.07|P-value:3.65E-7 ID:SGTC_1775|Compound:st081588|FD-Score:3.35|P-value:5.51E-4 ID:SGTC_1783|Compound:st039459|FD-Score:4.09|P-value:3.29E-5 ID:SGTC_1862|Compound:st057513|FD-Score:3.35|P-value:5.39E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.73|P-value:1.37E-4 ID:SGTC_1887|Compound:st057648|FD-Score:-4.92|P-value:6.93E-7 ID:SGTC_1895|Compound:st058434|FD-Score:-3.84|P-value:8.40E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.44|P-value:3.92E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-4.36|P-value:9.59E-6 ID:SGTC_1904|Compound:st060225|FD-Score:3.82|P-value:9.49E-5 ID:SGTC_1967|Compound:st070177|FD-Score:-4.22|P-value:1.78E-5 ID:SGTC_1978|Compound:st071761|FD-Score:3.24|P-value:7.87E-4 ID:SGTC_2084|Compound:5306078|FD-Score:3.85|P-value:8.48E-5 ID:SGTC_2094|Compound:5332512|FD-Score:4.45|P-value:6.85E-6 ID:SGTC_2125|Compound:5246583|FD-Score:-3.17|P-value:9.37E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-4.71|P-value:1.92E-6 ID:SGTC_2310|Compound:7668677|FD-Score:4.10|P-value:3.13E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-3.67|P-value:1.62E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.55|P-value:2.62E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.61|P-value:1.98E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.57|P-value:4.12E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.41|P-value:8.42E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.66|P-value:1.80E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.55|P-value:4.48E-6 ID:SGTC_2966|Compound:9084041|FD-Score:-3.66|P-value:1.63E-4 ID:SGTC_3029|Compound:9088683|FD-Score:3.44|P-value:3.94E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.32|P-value:5.59E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.50|P-value:3.22E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.26|P-value:7.47E-4 ID:SGTC_3166|Compound:9102297|FD-Score:4.28|P-value:1.47E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.56|P-value:2.43E-4 ID:SGTC_3345|Compound:9148410|FD-Score:3.31|P-value:6.30E-4

Top fitness defect scores for YNL258C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1329 1447-1697 5.88 50.6 µM 4.62E-9 1 2.04E-9 1.55
2 SGTC_1469 k284-2957 5.07 126.0 µM 3.65E-7 7 1.97E-7 0.06
3 SGTC_2593 nobiletin 4.57 86.2 µM 4.12E-6 6 2.47E-6 0.12
4 SGTC_2827 7993847 4.55 71.4 µM 4.48E-6 4 2.70E-6 0.11
5 SGTC_2094 5332512 4.45 191.7 µM 6.85E-6 2 4.21E-6 0.36
6 SGTC_2601 securinine 4.41 100.0 µM 8.42E-6 14 5.22E-6 0.09
7 SGTC_3166 9102297 4.28 49.5 µM 1.47E-5 Golgi 3 9.36E-6 0.52
8 SGTC_1454 4239-0054 4.19 42.6 µM 2.17E-5 5 1.40E-5 0.02
9 SGTC_930 2810-3379 4.18 265.0 µM 2.27E-5 2 1.47E-5 0.09
10 SGTC_2310 7668677 4.10 200.0 µM 3.13E-5 5 2.05E-5 0.04
11 SGTC_1783 st039459 4.09 87.5 µM 3.29E-5 Golgi 9 2.17E-5 0.33
12 SGTC_1156 4092-0367 4.01 4.9 µM 4.59E-5 2 3.07E-5 0.03
13 SGTC_883 0850-0245 3.88 24.6 µM 7.72E-5 cell wall 6 5.28E-5 0.08
14 SGTC_2084 5306078 3.85 60.6 µM 8.48E-5 3 5.83E-5 0.15
15 SGTC_1904 st060225 3.82 78.0 µM 9.49E-5 5 6.55E-5 0.02
16 SGTC_1881 5652484 3.73 16.0 µM 1.37E-4 TRP & mitochondrial translation 8 9.64E-5 0.15
17 SGTC_878 0828-0289 3.72 13.7 µM 1.44E-4 22 1.02E-4 0.21
18 SGTC_2779 methotrexate 3.66 400.0 µM 1.80E-4 17 1.28E-4 0.01
19 SGTC_2544 sphondin 3.55 68.5 µM 2.62E-4 RNA pol III & RNase P/MRP 43 1.89E-4 0.09
20 SGTC_3069 9117009 3.50 49.5 µM 3.22E-4 60S ribosome export 4 2.35E-4 0.18

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.83E-13 YNL332W THI12 hom Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13
0.112 8.93E-11 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.111 1.15E-10 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.091 1.34E-7 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.090 1.73E-7 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.089 2.53E-7 YHL008C_p YHL008C_p hom Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.088 3.75E-7 YGR288W MAL13 hom MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
0.088 3.75E-7 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.083 1.36E-6 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.077 7.37E-6 YCR004C YCP4 hom Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.076 9.17E-6 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.076 1.08E-5 YDR079C-A TFB5 hom Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
0.075 1.40E-5 YNR071C_p YNR071C_p hom Putative aldose 1-epimerase
0.074 1.60E-5 YPL253C VIK1 hom Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p
0.074 1.73E-5 YJR039W_p YJR039W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies