YNL281W / HCH1

Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL281W / HCH1

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:3.02|P-value:2.66E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.59|P-value:1.96E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.87|P-value:5.86E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.45|P-value:3.80E-5 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.16|P-value:1.77E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.15|P-value:1.55E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.88|P-value:4.53E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.39|P-value:6.48E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.65|P-value:5.11E-8 ID:SGTC_769|Compound:0302-0231|FD-Score:3.21|P-value:1.18E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:2.78|P-value:7.18E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:4.45|P-value:1.80E-7 ID:SGTC_984|Compound:1492-0255|FD-Score:3.08|P-value:2.10E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:2.71|P-value:9.38E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:2.77|P-value:7.51E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.83|P-value:5.93E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.58|P-value:8.05E-8 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-2.84|P-value:6.74E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.11|P-value:1.79E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:2.26E-4 ID:SGTC_1836|Compound:st055642|FD-Score:2.74|P-value:8.23E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.87|P-value:1.31E-8 ID:SGTC_1961|Compound:st076550|FD-Score:2.96|P-value:3.46E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-2.92|P-value:4.84E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.11|P-value:1.80E-4 ID:SGTC_2019|Compound:4016004|FD-Score:3.58|P-value:2.05E-5 ID:SGTC_2149|Compound:5554786|FD-Score:4.02|P-value:2.08E-6 ID:SGTC_2283|Compound:7944793|FD-Score:-3.37|P-value:6.97E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.97|P-value:8.43E-16 ID:SGTC_2324|Compound:6149169|FD-Score:3.29|P-value:7.97E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.03|P-value:3.08E-4 ID:SGTC_2373|Compound:9071805|FD-Score:3.12|P-value:1.71E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:2.77|P-value:7.37E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.16|P-value:1.43E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.69|P-value:1.19E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-2.78|P-value:8.39E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.84|P-value:5.43E-6 ID:SGTC_3054|Compound:9094397|FD-Score:6.08|P-value:1.93E-12 ID:SGTC_3062|Compound:9077494|FD-Score:3.06|P-value:2.24E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.04|P-value:2.95E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.19|P-value:1.29E-4 ID:SGTC_3145|Compound:9085516|FD-Score:6.34|P-value:2.21E-13 ID:SGTC_3213|Compound:9129884|FD-Score:5.05|P-value:3.96E-9 ID:SGTC_3219|Compound:9131112|FD-Score:2.93|P-value:3.89E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.60|P-value:1.83E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:3.02|P-value:2.66E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.59|P-value:1.96E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.87|P-value:5.86E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.45|P-value:3.80E-5 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.16|P-value:1.77E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.15|P-value:1.55E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.88|P-value:4.53E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.39|P-value:6.48E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.65|P-value:5.11E-8 ID:SGTC_769|Compound:0302-0231|FD-Score:3.21|P-value:1.18E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:2.78|P-value:7.18E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:4.45|P-value:1.80E-7 ID:SGTC_984|Compound:1492-0255|FD-Score:3.08|P-value:2.10E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:2.71|P-value:9.38E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:2.77|P-value:7.51E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.83|P-value:5.93E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.58|P-value:8.05E-8 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-2.84|P-value:6.74E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.11|P-value:1.79E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:2.26E-4 ID:SGTC_1836|Compound:st055642|FD-Score:2.74|P-value:8.23E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.87|P-value:1.31E-8 ID:SGTC_1961|Compound:st076550|FD-Score:2.96|P-value:3.46E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-2.92|P-value:4.84E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.11|P-value:1.80E-4 ID:SGTC_2019|Compound:4016004|FD-Score:3.58|P-value:2.05E-5 ID:SGTC_2149|Compound:5554786|FD-Score:4.02|P-value:2.08E-6 ID:SGTC_2283|Compound:7944793|FD-Score:-3.37|P-value:6.97E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.97|P-value:8.43E-16 ID:SGTC_2324|Compound:6149169|FD-Score:3.29|P-value:7.97E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.03|P-value:3.08E-4 ID:SGTC_2373|Compound:9071805|FD-Score:3.12|P-value:1.71E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:2.77|P-value:7.37E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.16|P-value:1.43E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.69|P-value:1.19E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-2.78|P-value:8.39E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.84|P-value:5.43E-6 ID:SGTC_3054|Compound:9094397|FD-Score:6.08|P-value:1.93E-12 ID:SGTC_3062|Compound:9077494|FD-Score:3.06|P-value:2.24E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.04|P-value:2.95E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.19|P-value:1.29E-4 ID:SGTC_3145|Compound:9085516|FD-Score:6.34|P-value:2.21E-13 ID:SGTC_3213|Compound:9129884|FD-Score:5.05|P-value:3.96E-9 ID:SGTC_3219|Compound:9131112|FD-Score:2.93|P-value:3.89E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.60|P-value:1.83E-5

Top fitness defect scores for YNL281W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2292 7671323 6.97 168.1 µM 8.43E-16 RNA processing & uracil transport 3 1.55E-12
2 SGTC_3145 9085516 6.34 49.5 µM 2.21E-13 fatty acid desaturase (OLE1) 5 1.15E-10
3 SGTC_3054 9094397 6.08 49.5 µM 1.93E-12 fatty acid desaturase (OLE1) 8 6.13E-10
4 SGTC_3213 9129884 5.05 49.5 µM 3.96E-9 6 2.24E-7
5 SGTC_1958 st076513 4.87 41.9 µM 1.31E-8 60S ribosome export 16 5.66E-7
6 SGTC_663 1486-0733 4.65 13.9 µM 5.11E-8 cell wall signaling 35 1.63E-6
7 SGTC_1567 st069303 4.58 68.4 µM 8.05E-8 mitochondrial processes 7 2.32E-6
8 SGTC_937 3122-0005 4.45 46.7 µM 1.80E-7 16 4.33E-6
9 SGTC_2149 5554786 4.02 194.8 µM 2.08E-6 cell wall signaling 36 2.89E-5
10 SGTC_473 2-arachidonoylglycerol 3.88 10.0 µM 4.53E-6 21 5.29E-5
11 SGTC_3000 9075062 3.84 71.4 µM 5.43E-6 21 6.10E-5
12 SGTC_2925 9010536 3.69 32.5 µM 1.19E-5 azole & statin 61 1.12E-4
13 SGTC_3330 9141424 3.60 71.4 µM 1.83E-5 amide catabolism 28 1.57E-4
14 SGTC_74 000s-0487 3.59 160.3 µM 1.96E-5 29 1.65E-4
15 SGTC_2019 4016004 3.58 108.0 µM 2.05E-5 41 1.71E-4
16 SGTC_89 0302-0250 3.45 142.1 µM 3.80E-5 40 2.77E-4
17 SGTC_2324 6149169 3.29 200.0 µM 7.97E-5 33 4.93E-4
18 SGTC_769 0302-0231 3.21 76.0 µM 1.18E-4 97 6.71E-4
19 SGTC_3128 9127691 3.19 49.5 µM 1.29E-4 41 7.19E-4
20 SGTC_2842 9000675 3.16 19.5 µM 1.43E-4 52 7.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.15E-12 YIL132C CSM2 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
0.108 4.01E-10 YCR014C POL4 hom DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
0.102 3.24E-9 YGR069W_d YGR069W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.101 5.11E-9 YJR126C VPS70 hom Protein of unknown function involved in vacuolar protein sorting
0.101 5.26E-9 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.094 5.66E-8 YKL071W_p YKL071W_p hom Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.094 5.68E-8 YIL061C SNP1 het Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
0.091 1.11E-7 YNL230C ELA1 hom Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
0.091 1.50E-7 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.087 4.10E-7 YBL106C SRO77 hom Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
0.087 4.54E-7 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.087 5.13E-7 YPL211W NIP7 het Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
0.086 5.60E-7 YKR075C_p YKR075C_p hom Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.085 8.11E-7 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.084 1.01E-6 YNL303W_d YNL303W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data