YNL291C / MID1

N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL291C / MID1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:9.74|P-value:2.37E-28 ID:SGTC_65|Compound:0798-0905|FD-Score:3.77|P-value:1.10E-5 ID:SGTC_111|Compound:0083-0039|FD-Score:6.62|P-value:4.10E-14 ID:SGTC_118|Compound:0083-0097|FD-Score:-3.50|P-value:3.78E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:2.92|P-value:5.13E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.59|P-value:2.66E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:2.86|P-value:6.42E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.27|P-value:7.68E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:5.37|P-value:7.31E-10 ID:SGTC_310|Compound:1273-0058|FD-Score:4.57|P-value:1.28E-7 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-2.93|P-value:4.62E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.84|P-value:6.90E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.89|P-value:5.76E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.94|P-value:4.43E-6 ID:SGTC_635|Compound:k038-0028|FD-Score:3.96|P-value:4.16E-6 ID:SGTC_668|Compound:1682-0499|FD-Score:3.73|P-value:1.30E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-2.96|P-value:4.02E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.86|P-value:6.31E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:4.97|P-value:1.07E-8 ID:SGTC_873|Compound:0591-4978|FD-Score:3.20|P-value:1.58E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.21|P-value:1.03E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.01|P-value:3.19E-6 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.34|P-value:8.34E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.12|P-value:1.70E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-2.82|P-value:7.19E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.05|P-value:2.97E-4 ID:SGTC_2184|Compound:6075246|FD-Score:2.89|P-value:5.59E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-2.97|P-value:3.84E-4 ID:SGTC_2328|Compound:6201408|FD-Score:3.61|P-value:2.43E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.76|P-value:1.12E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-2.86|P-value:6.00E-4 ID:SGTC_2375|Compound:9072683|FD-Score:6.41|P-value:2.50E-13 ID:SGTC_2382|Compound:9035899|FD-Score:3.03|P-value:3.21E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-2.73|P-value:9.99E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.21|P-value:1.43E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.33|P-value:8.89E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.84|P-value:2.40E-8 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-2.97|P-value:3.83E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.45|P-value:2.45E-7 ID:SGTC_2797|Compound:7859098|FD-Score:3.36|P-value:7.83E-5 ID:SGTC_3331|Compound:9141060|FD-Score:-2.77|P-value:8.77E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:9.74|P-value:2.37E-28 ID:SGTC_65|Compound:0798-0905|FD-Score:3.77|P-value:1.10E-5 ID:SGTC_111|Compound:0083-0039|FD-Score:6.62|P-value:4.10E-14 ID:SGTC_118|Compound:0083-0097|FD-Score:-3.50|P-value:3.78E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:2.92|P-value:5.13E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.59|P-value:2.66E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:2.86|P-value:6.42E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.27|P-value:7.68E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:5.37|P-value:7.31E-10 ID:SGTC_310|Compound:1273-0058|FD-Score:4.57|P-value:1.28E-7 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-2.93|P-value:4.62E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.84|P-value:6.90E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.89|P-value:5.76E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.94|P-value:4.43E-6 ID:SGTC_635|Compound:k038-0028|FD-Score:3.96|P-value:4.16E-6 ID:SGTC_668|Compound:1682-0499|FD-Score:3.73|P-value:1.30E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-2.96|P-value:4.02E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.86|P-value:6.31E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:4.97|P-value:1.07E-8 ID:SGTC_873|Compound:0591-4978|FD-Score:3.20|P-value:1.58E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.21|P-value:1.03E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.01|P-value:3.19E-6 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.34|P-value:8.34E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.12|P-value:1.70E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-2.82|P-value:7.19E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.05|P-value:2.97E-4 ID:SGTC_2184|Compound:6075246|FD-Score:2.89|P-value:5.59E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-2.97|P-value:3.84E-4 ID:SGTC_2328|Compound:6201408|FD-Score:3.61|P-value:2.43E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.76|P-value:1.12E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-2.86|P-value:6.00E-4 ID:SGTC_2375|Compound:9072683|FD-Score:6.41|P-value:2.50E-13 ID:SGTC_2382|Compound:9035899|FD-Score:3.03|P-value:3.21E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-2.73|P-value:9.99E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.21|P-value:1.43E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.33|P-value:8.89E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.84|P-value:2.40E-8 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-2.97|P-value:3.83E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.45|P-value:2.45E-7 ID:SGTC_2797|Compound:7859098|FD-Score:3.36|P-value:7.83E-5 ID:SGTC_3331|Compound:9141060|FD-Score:-2.77|P-value:8.77E-4

Top fitness defect scores for YNL291C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 9.74 9.0 µM 2.37E-28 cell wall 85 1.07E-22
2 SGTC_111 0083-0039 6.62 52.5 µM 4.10E-14 1 1.77E-11
3 SGTC_2375 9072683 6.41 200.0 µM 2.50E-13 mitochondrial processes 2 7.36E-11
4 SGTC_299 1273-0059 5.37 1.4 µM 7.31E-10 8 4.03E-8
5 SGTC_810 3825-7150 4.97 143.0 µM 1.07E-8 60S ribosome export 2 3.37E-7
6 SGTC_2582 agaric acid 4.84 100.0 µM 2.40E-8 redox potentiating 231 6.40E-7
7 SGTC_310 1273-0058 4.57 1.1 µM 1.28E-7 mitochondrial stress 205 2.41E-6
8 SGTC_255 tunicamycin 4.27 200.5 nM 7.68E-7 unfolded protein response 63 9.98E-6
9 SGTC_1083 didemethylchlorpromazine sulfoxide 4.21 707.4 nM 1.03E-6 redox potentiating 64 1.26E-5
10 SGTC_1585 2',4'-dihydroxychalcone 4.12 10.4 µM 1.70E-6 27 1.88E-5
11 SGTC_1203 3992-3522 4.01 103.0 µM 3.19E-6 29 3.09E-5
12 SGTC_635 k038-0028 3.96 24.7 µM 4.16E-6 ubiquinone biosynthesis & proteasome 44 3.82E-5
13 SGTC_634 1611-4317 3.94 11.2 µM 4.43E-6 ERG2 15 4.01E-5
14 SGTC_65 0798-0905 3.77 35.0 µM 1.10E-5 calcium & mitochondrial duress 97 8.29E-5
15 SGTC_2333 9017922 3.76 200.0 µM 1.12E-5 33 8.39E-5
16 SGTC_668 1682-0499 3.73 62.6 µM 1.30E-5 24 9.43E-5
17 SGTC_2328 6201408 3.61 200.0 µM 2.43E-5 29 1.55E-4
18 SGTC_185 k048-0037 3.59 25.5 µM 2.66E-5 RSC & ERG11 16 1.67E-4
19 SGTC_2797 7859098 3.36 81.8 µM 7.83E-5 48 3.95E-4
20 SGTC_1395 3486-0190 3.34 4.3 µM 8.34E-5 35 4.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.438 7.83E-157 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.339 6.82E-91 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.315 6.80E-78 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.308 9.22E-75 YIL123W SIM1 hom Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
0.300 1.74E-70 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.288 3.66E-65 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.286 2.78E-64 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.263 2.72E-54 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.263 4.11E-54 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.251 2.15E-49 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.249 1.94E-48 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.249 2.18E-48 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.246 1.65E-47 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.245 5.85E-47 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.240 5.78E-45 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress