YNL313C / EMW1

Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL313C / EMW1

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:3.15|P-value:3.57E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:-3.72|P-value:3.42E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:2.92|P-value:8.48E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.83|P-value:1.93E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.18|P-value:1.31E-8 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.56|P-value:6.93E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:3.52|P-value:7.94E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:4.28|P-value:2.14E-6 ID:SGTC_784|Compound:4579-0127|FD-Score:4.02|P-value:7.83E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:6.33|P-value:5.31E-12 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.08|P-value:4.79E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:2.89|P-value:9.40E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.22|P-value:3.00E-6 ID:SGTC_1681|Compound:tripelennamine|FD-Score:2.89|P-value:9.69E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.46|P-value:1.04E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.05|P-value:5.23E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.05|P-value:5.26E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.14|P-value:3.77E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.87|P-value:1.63E-5 ID:SGTC_2104|Compound:5389443|FD-Score:2.92|P-value:8.63E-4 ID:SGTC_2113|Compound:5491891|FD-Score:2.96|P-value:7.46E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.91|P-value:1.38E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.07|P-value:4.81E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.17|P-value:3.38E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.26|P-value:2.42E-6 ID:SGTC_2435|Compound:5706476|FD-Score:3.08|P-value:4.71E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:2.91|P-value:8.88E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.37|P-value:1.34E-6 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.68|P-value:3.88E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.42|P-value:1.25E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.66|P-value:4.35E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.67|P-value:3.99E-5 ID:SGTC_3123|Compound:9126162|FD-Score:-3.89|P-value:1.52E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-2.93|P-value:8.55E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.15|P-value:3.57E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:-3.72|P-value:3.42E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:2.92|P-value:8.48E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.83|P-value:1.93E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.18|P-value:1.31E-8 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.56|P-value:6.93E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:3.52|P-value:7.94E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:4.28|P-value:2.14E-6 ID:SGTC_784|Compound:4579-0127|FD-Score:4.02|P-value:7.83E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:6.33|P-value:5.31E-12 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.08|P-value:4.79E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:2.89|P-value:9.40E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.22|P-value:3.00E-6 ID:SGTC_1681|Compound:tripelennamine|FD-Score:2.89|P-value:9.69E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.46|P-value:1.04E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.05|P-value:5.23E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.05|P-value:5.26E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.14|P-value:3.77E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.87|P-value:1.63E-5 ID:SGTC_2104|Compound:5389443|FD-Score:2.92|P-value:8.63E-4 ID:SGTC_2113|Compound:5491891|FD-Score:2.96|P-value:7.46E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.91|P-value:1.38E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.07|P-value:4.81E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.17|P-value:3.38E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.26|P-value:2.42E-6 ID:SGTC_2435|Compound:5706476|FD-Score:3.08|P-value:4.71E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:2.91|P-value:8.88E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.37|P-value:1.34E-6 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.68|P-value:3.88E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.42|P-value:1.25E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.66|P-value:4.35E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.67|P-value:3.99E-5 ID:SGTC_3123|Compound:9126162|FD-Score:-3.89|P-value:1.52E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-2.93|P-value:8.55E-4

Top fitness defect scores for YNL313C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_879 0831-1030 6.33 162.0 µM 5.31E-12 4 1.19E-10 0.57
2 SGTC_513 4245-1575 5.18 41.7 µM 1.31E-8 unfolded protein response 16 1.08E-7 0.06
3 SGTC_2544 sphondin 4.37 68.5 µM 1.34E-6 RNA pol III & RNase P/MRP 33 6.14E-6 0.10
4 SGTC_611 3453-1652 4.28 23.7 µM 2.14E-6 17 9.24E-6 0.13
5 SGTC_2426 5582046 4.26 32.2 µM 2.42E-6 5 1.03E-5 0.02
6 SGTC_1585 2',4'-dihydroxychalcone 4.22 10.4 µM 3.00E-6 8 1.24E-5 0.07
7 SGTC_784 4579-0127 4.02 173.0 µM 7.83E-6 3 2.87E-5 0.11
8 SGTC_2135 5326629 3.91 150.4 µM 1.38E-5 RPP1 & pyrimidine depletion 6 4.71E-5 0.19
9 SGTC_1987 st072290 3.87 61.9 µM 1.63E-5 2 5.43E-5 0.01
10 SGTC_485 niflumic acid 3.83 177.0 µM 1.93E-5 1 6.31E-5 0.20
11 SGTC_2749 bexarotene 3.68 57.4 µM 3.88E-5 7 1.16E-4 0.04
12 SGTC_2788 5282734 3.67 71.4 µM 3.99E-5 11 1.19E-4 0.03
13 SGTC_524 r073-0010 3.52 27.8 µM 7.94E-5 19 2.17E-4 0.03
14 SGTC_1704 st028805 3.46 70.8 µM 1.04E-4 9 2.75E-4 0.26
15 SGTC_2327 6189656 3.17 200.0 µM 3.38E-4 19 7.72E-4 0.02
16 SGTC_115 0527-0192 3.15 112.0 µM 3.57E-4 41 8.11E-4 0.11
17 SGTC_2435 5706476 3.08 10.6 µM 4.71E-4 6 0.00103 0.06
18 SGTC_2137 5261020 3.07 140.0 µM 4.81E-4 17 0.00105 0.04
19 SGTC_1742 st038235 3.05 28.9 µM 5.23E-4 mitochondrial stress 20 0.00113 0.04
20 SGTC_1916 st067606 3.05 49.2 µM 5.26E-4 17 0.00114 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.49E-16 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.137 1.99E-15 YPL010W RET3 het Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.116 1.39E-11 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.114 3.98E-11 YCR095W-A_p YCR095W-A_p hom Putative protein of unknown function
0.113 5.58E-11 YLL001W DNM1 hom Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance
0.106 6.98E-10 YMR185W RTP1 het Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
0.106 8.20E-10 YDR249C_p YDR249C_p hom Putative protein of unknown function
0.102 2.84E-9 YKR045C_p YKR045C_p hom Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
0.100 6.95E-9 YMR155W_p YMR155W_p hom Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen
0.098 1.48E-8 YDL208W NHP2 het Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
0.096 2.85E-8 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.096 2.96E-8 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.094 5.50E-8 YOR262W GPN2 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
0.091 1.41E-7 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.090 1.78E-7 YDR219C MFB1 hom Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding