YNL328C / MDJ2

Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL328C / MDJ2

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:3.90|P-value:1.33E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:-3.78|P-value:1.66E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.50|P-value:5.40E-4 ID:SGTC_448|Compound:monastrol|FD-Score:5.71|P-value:4.46E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:5.02|P-value:1.30E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.31|P-value:8.29E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.55|P-value:3.79E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.92|P-value:1.25E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.85|P-value:1.30E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.41|P-value:5.98E-4 ID:SGTC_2332|Compound:7982454|FD-Score:5.22|P-value:5.00E-7 ID:SGTC_2337|Compound:9009472|FD-Score:-4.80|P-value:2.73E-6 ID:SGTC_2421|Compound:5690543|FD-Score:-4.60|P-value:6.45E-6 ID:SGTC_2425|Compound:5628681|FD-Score:6.43|P-value:8.38E-10 ID:SGTC_2430|Compound:5571206|FD-Score:-3.33|P-value:7.74E-4 ID:SGTC_2435|Compound:5706476|FD-Score:-3.71|P-value:2.19E-4 ID:SGTC_3315|Compound:9136395|FD-Score:-3.53|P-value:4.03E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.90|P-value:1.33E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:-3.78|P-value:1.66E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.50|P-value:5.40E-4 ID:SGTC_448|Compound:monastrol|FD-Score:5.71|P-value:4.46E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:5.02|P-value:1.30E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.31|P-value:8.29E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.55|P-value:3.79E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.92|P-value:1.25E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.85|P-value:1.30E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.41|P-value:5.98E-4 ID:SGTC_2332|Compound:7982454|FD-Score:5.22|P-value:5.00E-7 ID:SGTC_2337|Compound:9009472|FD-Score:-4.80|P-value:2.73E-6 ID:SGTC_2421|Compound:5690543|FD-Score:-4.60|P-value:6.45E-6 ID:SGTC_2425|Compound:5628681|FD-Score:6.43|P-value:8.38E-10 ID:SGTC_2430|Compound:5571206|FD-Score:-3.33|P-value:7.74E-4 ID:SGTC_2435|Compound:5706476|FD-Score:-3.71|P-value:2.19E-4 ID:SGTC_3315|Compound:9136395|FD-Score:-3.53|P-value:4.03E-4

Top fitness defect scores for YNL328C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2425 5628681 6.43 7.8 µM 8.38E-10 1 6.37E-11
2 SGTC_448 monastrol 5.71 171.0 µM 4.46E-8 3 5.68E-9
3 SGTC_2332 7982454 5.22 138.9 µM 5.00E-7 27 8.72E-8
4 SGTC_542 3381-0244 5.02 89.3 µM 1.30E-6 19 2.57E-7
5 SGTC_1850 st056238 3.92 17.0 µM 1.25E-4 RPP1 & pyrimidine depletion 21 4.43E-5
6 SGTC_118 0083-0097 3.90 135.3 µM 1.33E-4 translation 138 4.77E-5
7 SGTC_237 nikkomycin 3.50 200.0 µM 5.40E-4 RSC complex & mRNA processing 26 2.32E-4
8 SGTC_1157 4092-0394 3.28 27.6 µM 0.00109 49 5.14E-4
9 SGTC_300 r073-0011 3.28 5.0 µM 0.00109 43 5.14E-4
10 SGTC_1840 st055363 3.27 71.0 µM 0.00115 34 5.41E-4
11 SGTC_949 1165-0505 3.21 146.0 µM 0.00137 53 6.61E-4
12 SGTC_868 0527-0199 3.12 213.0 µM 0.00179 fatty acid desaturase (OLE1) 58 8.92E-4
13 SGTC_2694 st077685 3.10 81.9 µM 0.00193 endomembrane recycling 63 9.72E-4
14 SGTC_121 0557-0495 2.98 61.3 µM 0.00272 calcium & mitochondrial duress 137 0.00143
15 SGTC_298 1391-0729 2.96 10.0 µM 0.00287 calcium & mitochondrial duress 42 0.00152
16 SGTC_734 2023-0417 2.95 80.0 µM 0.00298 ergosterol biosynthesis 78 0.00159
17 SGTC_2240 6690706 2.93 185.9 µM 0.00316 47 0.00170
18 SGTC_2058 5236698 2.92 133.0 µM 0.00329 78 0.00177
19 SGTC_2091 4-phenylthiadiazole 2.89 200.0 µM 0.00357 57 0.00195
20 SGTC_2092 5303694 2.88 80.7 µM 0.00367 TSC3-RPN4 91 0.00201

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.45E-17 YGR278W CWC22 het Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
0.134 5.32E-15 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.108 4.37E-10 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.095 3.71E-8 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.090 1.58E-7 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.090 1.84E-7 YER076C_p YER076C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
0.089 2.52E-7 YLR084C RAX2 hom N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
0.086 5.38E-7 YGR014W MSB2 hom Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate
0.086 6.49E-7 YEL033W_p MTC7_p hom Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
0.085 7.12E-7 YDL213C NOP6 hom rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
0.085 7.33E-7 YIR007W_p YIR007W_p hom Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
0.085 8.03E-7 YDR146C SWI5 hom Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
0.084 1.03E-6 YJR099W YUH1 hom Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
0.084 1.06E-6 YOR107W RGS2 hom Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
0.084 1.20E-6 YOL131W_p YOL131W_p hom Putative protein of unknown function