YNL332W / THI12

Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL332W / THI12

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.00|P-value:7.97E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:3.83|P-value:2.89E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.86|P-value:3.27E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:3.40|P-value:1.77E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.30|P-value:2.58E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:4.64|P-value:5.64E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:4.25|P-value:4.06E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.64|P-value:6.47E-5 ID:SGTC_851|Compound:0394-0702|FD-Score:3.04|P-value:6.90E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.25|P-value:3.10E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:4.41|P-value:1.87E-6 ID:SGTC_986|Compound:1493-0483|FD-Score:3.22|P-value:3.53E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.06|P-value:9.88E-6 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.51|P-value:1.12E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:5.21|P-value:2.41E-8 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.67|P-value:5.88E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:-3.76|P-value:4.98E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:-3.08|P-value:7.41E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.38|P-value:1.93E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.65|P-value:6.21E-5 ID:SGTC_1660|Compound:st012960|FD-Score:3.22|P-value:3.54E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.31|P-value:3.09E-6 ID:SGTC_1741|Compound:st038134|FD-Score:3.00|P-value:7.86E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.48|P-value:1.29E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.13|P-value:4.99E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.54|P-value:9.95E-5 ID:SGTC_1893|Compound:st059353|FD-Score:5.69|P-value:1.23E-9 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.04|P-value:6.94E-4 ID:SGTC_1903|Compound:st060211|FD-Score:4.11|P-value:8.01E-6 ID:SGTC_1904|Compound:st060225|FD-Score:3.88|P-value:2.28E-5 ID:SGTC_1905|Compound:st060207|FD-Score:-4.17|P-value:8.07E-6 ID:SGTC_2014|Compound:4013930|FD-Score:2.94|P-value:9.91E-4 ID:SGTC_2020|Compound:4017018|FD-Score:2.99|P-value:8.27E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.22|P-value:3.54E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-4.19|P-value:7.15E-6 ID:SGTC_2329|Compound:6138490|FD-Score:-3.00|P-value:9.84E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.01|P-value:7.76E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.67|P-value:5.80E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.95|P-value:9.43E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.00|P-value:8.06E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.97|P-value:1.56E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.43|P-value:1.55E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.14|P-value:5.99E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:8.39|P-value:8.65E-19 ID:SGTC_2623|Compound:humulene|FD-Score:3.33|P-value:2.31E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.79|P-value:6.38E-10 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.35|P-value:2.73E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.77|P-value:3.79E-5 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.34|P-value:2.20E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.19|P-value:4.91E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.01|P-value:7.79E-4 ID:SGTC_2861|Compound:9024595|FD-Score:3.00|P-value:7.88E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-4.09|P-value:1.14E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.69|P-value:6.85E-5 ID:SGTC_3016|Compound:9080866|FD-Score:-3.30|P-value:3.24E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.39|P-value:1.85E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-3.08|P-value:7.47E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.76|P-value:3.84E-5 ID:SGTC_3139|Compound:9095020|FD-Score:3.06|P-value:6.36E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.00|P-value:7.97E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:3.83|P-value:2.89E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.86|P-value:3.27E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:3.40|P-value:1.77E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.30|P-value:2.58E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:4.64|P-value:5.64E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:4.25|P-value:4.06E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.64|P-value:6.47E-5 ID:SGTC_851|Compound:0394-0702|FD-Score:3.04|P-value:6.90E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.25|P-value:3.10E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:4.41|P-value:1.87E-6 ID:SGTC_986|Compound:1493-0483|FD-Score:3.22|P-value:3.53E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.06|P-value:9.88E-6 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.51|P-value:1.12E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:5.21|P-value:2.41E-8 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.67|P-value:5.88E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:-3.76|P-value:4.98E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:-3.08|P-value:7.41E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.38|P-value:1.93E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.65|P-value:6.21E-5 ID:SGTC_1660|Compound:st012960|FD-Score:3.22|P-value:3.54E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.31|P-value:3.09E-6 ID:SGTC_1741|Compound:st038134|FD-Score:3.00|P-value:7.86E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.48|P-value:1.29E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.13|P-value:4.99E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.54|P-value:9.95E-5 ID:SGTC_1893|Compound:st059353|FD-Score:5.69|P-value:1.23E-9 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.04|P-value:6.94E-4 ID:SGTC_1903|Compound:st060211|FD-Score:4.11|P-value:8.01E-6 ID:SGTC_1904|Compound:st060225|FD-Score:3.88|P-value:2.28E-5 ID:SGTC_1905|Compound:st060207|FD-Score:-4.17|P-value:8.07E-6 ID:SGTC_2014|Compound:4013930|FD-Score:2.94|P-value:9.91E-4 ID:SGTC_2020|Compound:4017018|FD-Score:2.99|P-value:8.27E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.22|P-value:3.54E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-4.19|P-value:7.15E-6 ID:SGTC_2329|Compound:6138490|FD-Score:-3.00|P-value:9.84E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.01|P-value:7.76E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.67|P-value:5.80E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.95|P-value:9.43E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.00|P-value:8.06E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.97|P-value:1.56E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.43|P-value:1.55E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.14|P-value:5.99E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:8.39|P-value:8.65E-19 ID:SGTC_2623|Compound:humulene|FD-Score:3.33|P-value:2.31E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.79|P-value:6.38E-10 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.35|P-value:2.73E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.77|P-value:3.79E-5 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.34|P-value:2.20E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.19|P-value:4.91E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.01|P-value:7.79E-4 ID:SGTC_2861|Compound:9024595|FD-Score:3.00|P-value:7.88E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-4.09|P-value:1.14E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.69|P-value:6.85E-5 ID:SGTC_3016|Compound:9080866|FD-Score:-3.30|P-value:3.24E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.39|P-value:1.85E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-3.08|P-value:7.47E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.76|P-value:3.84E-5 ID:SGTC_3139|Compound:9095020|FD-Score:3.06|P-value:6.36E-4

Top fitness defect scores for YNL332W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2622 2-methoxyxanthone 8.39 59.9 µM 8.65E-19 1 2.46E-17
2 SGTC_2626 ethyl orsellinate 5.79 100.0 µM 6.38E-10 7 3.42E-9
3 SGTC_1893 st059353 5.69 36.1 µM 1.23E-9 6 6.24E-9
4 SGTC_1329 1447-1697 5.21 50.6 µM 2.41E-8 6 9.61E-8
5 SGTC_608 k048-0125 4.64 159.0 µM 5.64E-7 16 1.74E-6
6 SGTC_980 1473-0395 4.41 201.0 µM 1.87E-6 4 5.21E-6
7 SGTC_1740 st038286 4.31 13.2 µM 3.09E-6 20 8.27E-6
8 SGTC_611 3453-1652 4.25 23.7 µM 4.06E-6 13 1.06E-5
9 SGTC_1903 st060211 4.11 78.3 µM 8.01E-6 22 1.98E-5
10 SGTC_1034 k072-0165 4.06 12.7 µM 9.88E-6 Golgi 19 2.40E-5
11 SGTC_2593 nobiletin 3.97 86.2 µM 1.56E-5 26 3.65E-5
12 SGTC_1904 st060225 3.88 78.0 µM 2.28E-5 23 5.17E-5
13 SGTC_49 0717-0965 3.83 32.1 µM 2.89E-5 60S ribosome export 22 6.44E-5
14 SGTC_2671 danthron 3.77 100.0 µM 3.79E-5 DNA damage response 49 8.26E-5
15 SGTC_3131 9127911 3.76 49.5 µM 3.84E-5 34 8.36E-5
16 SGTC_2437 5741531 3.67 144.2 µM 5.80E-5 45 1.22E-4
17 SGTC_1377 2944-0135 3.67 198.0 µM 5.88E-5 calcium & mitochondrial duress 47 1.24E-4
18 SGTC_1564 D-Arginine 3.65 115.0 µM 6.21E-5 37 1.30E-4
19 SGTC_769 0302-0231 3.64 76.0 µM 6.47E-5 52 1.35E-4
20 SGTC_1892 st058411 3.54 7.4 µM 9.95E-5 40 2.00E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.98E-14 YLR178C TFS1 hom Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
0.126 2.83E-13 YNL258C DSL1 het Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
0.097 1.54E-8 YCR004C YCP4 hom Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.086 6.39E-7 YLR454W_p FMP27_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.085 8.11E-7 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.084 9.72E-7 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
0.083 1.56E-6 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.080 3.49E-6 YBL032W HEK2 hom RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
0.074 1.78E-5 YOL091W SPO21 hom Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
0.073 2.11E-5 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.072 3.10E-5 YER163C GCG1 hom Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
0.072 3.18E-5 YPL183C RTT10 hom WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
0.071 3.91E-5 YOR339C UBC11 hom Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
0.071 4.00E-5 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.071 4.00E-5 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport