YNR018W / RCF2

Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR018W / RCF2

Click on Significant Values for Screen Details ID:SGTC_102|Compound:gallobenzophenone|FD-Score:2.94|P-value:9.94E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:3.34|P-value:2.14E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.95|P-value:9.51E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.20|P-value:3.72E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.37|P-value:1.88E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:4.34|P-value:2.40E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.02|P-value:6.60E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.30|P-value:2.96E-6 ID:SGTC_389|Compound:tpck|FD-Score:-4.06|P-value:8.38E-6 ID:SGTC_428|Compound:3448-4153|FD-Score:3.58|P-value:8.20E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.44|P-value:1.47E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.41|P-value:1.61E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.99|P-value:7.38E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:6.83|P-value:2.77E-13 ID:SGTC_736|Compound:0958-0254|FD-Score:3.00|P-value:7.97E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.12|P-value:5.11E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.80|P-value:3.03E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:3.66|P-value:5.78E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:3.39|P-value:1.78E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:6.31|P-value:1.41E-11 ID:SGTC_1093|Compound:fluphenazine|FD-Score:5.54|P-value:2.46E-9 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.07|P-value:8.81E-6 ID:SGTC_1243|Compound:0416-0003|FD-Score:6.20|P-value:3.03E-11 ID:SGTC_1249|Compound:0481-0015|FD-Score:5.08|P-value:4.20E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:-5.31|P-value:8.86E-9 ID:SGTC_1415|Compound:3966-0071|FD-Score:-2.96|P-value:8.28E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:4.69|P-value:3.69E-7 ID:SGTC_1438|Compound:4092-1045|FD-Score:-3.06|P-value:5.86E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.97|P-value:1.40E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.21|P-value:3.32E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.27|P-value:2.91E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.55|P-value:8.10E-5 ID:SGTC_1590|Compound:st077108|FD-Score:-3.13|P-value:4.44E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.14|P-value:5.68E-6 ID:SGTC_1635|Compound:st007035|FD-Score:38.90|P-value:0 ID:SGTC_1651|Compound:st011928|FD-Score:3.99|P-value:1.30E-5 ID:SGTC_1664|Compound:st014217|FD-Score:-3.03|P-value:6.42E-4 ID:SGTC_1757|Compound:st041620|FD-Score:3.47|P-value:1.27E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.31|P-value:2.47E-4 ID:SGTC_1979|Compound:st071809|FD-Score:2.97|P-value:8.69E-4 ID:SGTC_1992|Compound:st073643|FD-Score:3.54|P-value:9.65E-5 ID:SGTC_2045|Compound:5106652|FD-Score:-3.09|P-value:5.15E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.19|P-value:3.84E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.09|P-value:5.72E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.09|P-value:5.64E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-4.14|P-value:5.64E-6 ID:SGTC_2431|Compound:5490079|FD-Score:3.84|P-value:2.56E-5 ID:SGTC_2453|Compound:5731132|FD-Score:-3.32|P-value:2.12E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.17|P-value:4.24E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:2.95|P-value:9.38E-4 ID:SGTC_2825|Compound:7998094|FD-Score:-3.08|P-value:5.35E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.45|P-value:1.39E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-2.92|P-value:9.65E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.49|P-value:1.15E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.82|P-value:4.20E-10 ID:SGTC_3020|Compound:9089140|FD-Score:3.98|P-value:1.37E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.09|P-value:5.66E-4 ID:SGTC_3038|Compound:9091290|FD-Score:3.41|P-value:1.65E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.05|P-value:5.06E-8 ID:SGTC_3303|Compound:9125618|FD-Score:-2.96|P-value:8.28E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-2.99|P-value:7.36E-4 ID:SGTC_3322|Compound:9139058|FD-Score:3.04|P-value:6.89E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:2.94|P-value:9.94E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:3.34|P-value:2.14E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.95|P-value:9.51E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.20|P-value:3.72E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.37|P-value:1.88E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:4.34|P-value:2.40E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.02|P-value:6.60E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.30|P-value:2.96E-6 ID:SGTC_389|Compound:tpck|FD-Score:-4.06|P-value:8.38E-6 ID:SGTC_428|Compound:3448-4153|FD-Score:3.58|P-value:8.20E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.44|P-value:1.47E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.41|P-value:1.61E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.99|P-value:7.38E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:6.83|P-value:2.77E-13 ID:SGTC_736|Compound:0958-0254|FD-Score:3.00|P-value:7.97E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.12|P-value:5.11E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.80|P-value:3.03E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:3.66|P-value:5.78E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:3.39|P-value:1.78E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:6.31|P-value:1.41E-11 ID:SGTC_1093|Compound:fluphenazine|FD-Score:5.54|P-value:2.46E-9 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.07|P-value:8.81E-6 ID:SGTC_1243|Compound:0416-0003|FD-Score:6.20|P-value:3.03E-11 ID:SGTC_1249|Compound:0481-0015|FD-Score:5.08|P-value:4.20E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:-5.31|P-value:8.86E-9 ID:SGTC_1415|Compound:3966-0071|FD-Score:-2.96|P-value:8.28E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:4.69|P-value:3.69E-7 ID:SGTC_1438|Compound:4092-1045|FD-Score:-3.06|P-value:5.86E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.97|P-value:1.40E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.21|P-value:3.32E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.27|P-value:2.91E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.55|P-value:8.10E-5 ID:SGTC_1590|Compound:st077108|FD-Score:-3.13|P-value:4.44E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.14|P-value:5.68E-6 ID:SGTC_1635|Compound:st007035|FD-Score:38.90|P-value:0 ID:SGTC_1651|Compound:st011928|FD-Score:3.99|P-value:1.30E-5 ID:SGTC_1664|Compound:st014217|FD-Score:-3.03|P-value:6.42E-4 ID:SGTC_1757|Compound:st041620|FD-Score:3.47|P-value:1.27E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.31|P-value:2.47E-4 ID:SGTC_1979|Compound:st071809|FD-Score:2.97|P-value:8.69E-4 ID:SGTC_1992|Compound:st073643|FD-Score:3.54|P-value:9.65E-5 ID:SGTC_2045|Compound:5106652|FD-Score:-3.09|P-value:5.15E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.19|P-value:3.84E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.09|P-value:5.72E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.09|P-value:5.64E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-4.14|P-value:5.64E-6 ID:SGTC_2431|Compound:5490079|FD-Score:3.84|P-value:2.56E-5 ID:SGTC_2453|Compound:5731132|FD-Score:-3.32|P-value:2.12E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.17|P-value:4.24E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:2.95|P-value:9.38E-4 ID:SGTC_2825|Compound:7998094|FD-Score:-3.08|P-value:5.35E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.45|P-value:1.39E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-2.92|P-value:9.65E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.49|P-value:1.15E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.82|P-value:4.20E-10 ID:SGTC_3020|Compound:9089140|FD-Score:3.98|P-value:1.37E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.09|P-value:5.66E-4 ID:SGTC_3038|Compound:9091290|FD-Score:3.41|P-value:1.65E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.05|P-value:5.06E-8 ID:SGTC_3303|Compound:9125618|FD-Score:-2.96|P-value:8.28E-4 ID:SGTC_3304|Compound:9125678|FD-Score:-2.99|P-value:7.36E-4 ID:SGTC_3322|Compound:9139058|FD-Score:3.04|P-value:6.89E-4

Top fitness defect scores for YNR018W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1635 st007035 38.90 42.4 µM 0 3 0
2 SGTC_704 1310-0270 6.83 142.0 µM 2.77E-13 3 4.20E-12
3 SGTC_1031 k035-0009 6.31 16.0 µM 1.41E-11 4 1.43E-10
4 SGTC_1243 0416-0003 6.20 5.7 µM 3.03E-11 PDR1 5 2.85E-10
5 SGTC_2950 9072895 5.82 21.5 µM 4.20E-10 9 3.02E-9
6 SGTC_1093 fluphenazine 5.54 8.0 µM 2.46E-9 3 1.48E-8
7 SGTC_1249 0481-0015 5.08 10.2 µM 4.20E-8 12 1.90E-7
8 SGTC_3225 9130819 5.05 49.5 µM 5.06E-8 amide catabolism 9 2.24E-7
9 SGTC_1416 3966-0321 4.69 25.3 µM 3.69E-7 5 1.34E-6
10 SGTC_364 0083-0097 4.34 67.7 µM 2.40E-6 18 7.22E-6
11 SGTC_381 3461-2219 4.30 42.7 µM 2.96E-6 copper-dependent oxidative stress 15 8.72E-6
12 SGTC_1146 3579-1023 4.07 7.0 µM 8.81E-6 12 2.33E-5
13 SGTC_1651 st011928 3.99 86.1 µM 1.30E-5 RSC complex & mRNA processing 12 3.30E-5
14 SGTC_3020 9089140 3.98 49.5 µM 1.37E-5 29 3.47E-5
15 SGTC_1510 1123-0200 3.97 71.5 µM 1.40E-5 41 3.54E-5
16 SGTC_2431 5490079 3.84 200.0 µM 2.56E-5 41 6.11E-5
17 SGTC_777 1315-0167 3.80 517.0 µM 3.03E-5 Golgi 30 7.10E-5
18 SGTC_785 1270-0111 3.66 65.3 µM 5.78E-5 38 1.27E-4
19 SGTC_428 3448-4153 3.58 16.5 µM 8.20E-5 TSC3-RPN4 43 1.74E-4
20 SGTC_1992 st073643 3.54 23.3 µM 9.65E-5 mitochondrial processes 15 2.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.604 0 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.552 1.43E-267 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.507 2.22E-218 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.503 5.40E-215 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.497 1.56E-208 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.464 5.04E-179 YPL189C-A COA2 hom Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
0.459 4.38E-174 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.441 1.23E-159 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.410 2.47E-136 YLR038C COX12 hom Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
0.379 5.09E-115 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.372 1.00E-110 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.369 4.36E-109 YDL067C COX9 hom Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.369 1.59E-108 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
0.361 3.67E-104 YIL098C FMC1 hom Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
0.354 6.17E-100 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies