YOL013C / HRD1

Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL013C / HRD1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:4.40|P-value:2.41E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.07|P-value:2.31E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:2.84|P-value:6.11E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:-3.11|P-value:1.56E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.68|P-value:1.34E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:-2.83|P-value:5.17E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.63|P-value:1.30E-5 ID:SGTC_305|Compound:1682-2595|FD-Score:2.89|P-value:4.92E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:2.73|P-value:9.25E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.69|P-value:2.51E-11 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:2.72|P-value:9.77E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.05|P-value:2.02E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:7.10|P-value:2.11E-16 ID:SGTC_570|Compound:0987-0065|FD-Score:2.80|P-value:7.06E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:-2.79|P-value:5.99E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.58|P-value:2.17E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.90|P-value:3.80E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.12|P-value:1.86E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:4.41|P-value:2.37E-7 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.01|P-value:2.42E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.03|P-value:2.71E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:2.81|P-value:6.86E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.24|P-value:1.09E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.38|P-value:5.77E-5 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.54|P-value:1.08E-7 ID:SGTC_1088|Compound:bifonazole|FD-Score:2.97|P-value:3.63E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.17|P-value:1.49E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.96|P-value:3.10E-6 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.06|P-value:2.48E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:2.95|P-value:3.83E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.13|P-value:1.41E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:2.85|P-value:5.75E-4 ID:SGTC_1548|Compound:st061468|FD-Score:2.83|P-value:6.30E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:2.86|P-value:5.50E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.26|P-value:1.01E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.24|P-value:1.10E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.30|P-value:8.16E-5 ID:SGTC_1665|Compound:st014639|FD-Score:3.26|P-value:9.98E-5 ID:SGTC_1677|Compound:st019204|FD-Score:-2.70|P-value:8.61E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-2.79|P-value:6.06E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.17|P-value:1.48E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.21|P-value:1.25E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.36|P-value:6.30E-5 ID:SGTC_1912|Compound:st060209|FD-Score:6.98|P-value:6.49E-16 ID:SGTC_1916|Compound:st067606|FD-Score:6.40|P-value:1.18E-13 ID:SGTC_1978|Compound:st071761|FD-Score:-2.86|P-value:4.54E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-4.12|P-value:9.29E-7 ID:SGTC_2260|Compound:7964312|FD-Score:3.10|P-value:2.04E-4 ID:SGTC_2261|Compound:9010538|FD-Score:5.12|P-value:2.40E-9 ID:SGTC_2291|Compound:7917025|FD-Score:-3.00|P-value:2.52E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.54|P-value:2.03E-5 ID:SGTC_2320|Compound:7373003|FD-Score:2.95|P-value:3.91E-4 ID:SGTC_2324|Compound:6149169|FD-Score:5.94|P-value:5.39E-12 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.01|P-value:2.97E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.51|P-value:1.27E-7 ID:SGTC_2469|Compound:5655019|FD-Score:2.77|P-value:8.16E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:2.96|P-value:3.67E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.76|P-value:8.96E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.96|P-value:3.70E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-2.79|P-value:5.98E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.60|P-value:1.48E-34 ID:SGTC_2584|Compound:androsterone|FD-Score:5.32|P-value:5.71E-10 ID:SGTC_2585|Compound:alliin|FD-Score:-2.86|P-value:4.47E-4 ID:SGTC_2713|Compound:st079149|FD-Score:-3.38|P-value:4.61E-5 ID:SGTC_2745|Compound:rifampicin|FD-Score:3.22|P-value:1.18E-4 ID:SGTC_2932|Compound:9016711|FD-Score:2.82|P-value:6.55E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.24|P-value:8.82E-5 ID:SGTC_2958|Compound:9077254|FD-Score:3.85|P-value:5.56E-6 ID:SGTC_2996|Compound:9066192|FD-Score:4.00|P-value:2.50E-6 ID:SGTC_3030|Compound:9087619|FD-Score:2.84|P-value:6.04E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.28|P-value:3.77E-7 ID:SGTC_3195|Compound:9113082|FD-Score:2.83|P-value:6.30E-4 ID:SGTC_3289|Compound:9119912|FD-Score:3.43|P-value:4.61E-5 ID:SGTC_3324|Compound:9138759|FD-Score:2.91|P-value:4.66E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.40|P-value:2.41E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.07|P-value:2.31E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:2.84|P-value:6.11E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:-3.11|P-value:1.56E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.68|P-value:1.34E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:-2.83|P-value:5.17E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.63|P-value:1.30E-5 ID:SGTC_305|Compound:1682-2595|FD-Score:2.89|P-value:4.92E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:2.73|P-value:9.25E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.69|P-value:2.51E-11 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:2.72|P-value:9.77E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.05|P-value:2.02E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:7.10|P-value:2.11E-16 ID:SGTC_570|Compound:0987-0065|FD-Score:2.80|P-value:7.06E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:-2.79|P-value:5.99E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.58|P-value:2.17E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.90|P-value:3.80E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.12|P-value:1.86E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:4.41|P-value:2.37E-7 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.01|P-value:2.42E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.03|P-value:2.71E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:2.81|P-value:6.86E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.24|P-value:1.09E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.38|P-value:5.77E-5 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.54|P-value:1.08E-7 ID:SGTC_1088|Compound:bifonazole|FD-Score:2.97|P-value:3.63E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.17|P-value:1.49E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.96|P-value:3.10E-6 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.06|P-value:2.48E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:2.95|P-value:3.83E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.13|P-value:1.41E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:2.85|P-value:5.75E-4 ID:SGTC_1548|Compound:st061468|FD-Score:2.83|P-value:6.30E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:2.86|P-value:5.50E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.26|P-value:1.01E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.24|P-value:1.10E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.30|P-value:8.16E-5 ID:SGTC_1665|Compound:st014639|FD-Score:3.26|P-value:9.98E-5 ID:SGTC_1677|Compound:st019204|FD-Score:-2.70|P-value:8.61E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-2.79|P-value:6.06E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.17|P-value:1.48E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.21|P-value:1.25E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.36|P-value:6.30E-5 ID:SGTC_1912|Compound:st060209|FD-Score:6.98|P-value:6.49E-16 ID:SGTC_1916|Compound:st067606|FD-Score:6.40|P-value:1.18E-13 ID:SGTC_1978|Compound:st071761|FD-Score:-2.86|P-value:4.54E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-4.12|P-value:9.29E-7 ID:SGTC_2260|Compound:7964312|FD-Score:3.10|P-value:2.04E-4 ID:SGTC_2261|Compound:9010538|FD-Score:5.12|P-value:2.40E-9 ID:SGTC_2291|Compound:7917025|FD-Score:-3.00|P-value:2.52E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.54|P-value:2.03E-5 ID:SGTC_2320|Compound:7373003|FD-Score:2.95|P-value:3.91E-4 ID:SGTC_2324|Compound:6149169|FD-Score:5.94|P-value:5.39E-12 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.01|P-value:2.97E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.51|P-value:1.27E-7 ID:SGTC_2469|Compound:5655019|FD-Score:2.77|P-value:8.16E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:2.96|P-value:3.67E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.76|P-value:8.96E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.96|P-value:3.70E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-2.79|P-value:5.98E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.60|P-value:1.48E-34 ID:SGTC_2584|Compound:androsterone|FD-Score:5.32|P-value:5.71E-10 ID:SGTC_2585|Compound:alliin|FD-Score:-2.86|P-value:4.47E-4 ID:SGTC_2713|Compound:st079149|FD-Score:-3.38|P-value:4.61E-5 ID:SGTC_2745|Compound:rifampicin|FD-Score:3.22|P-value:1.18E-4 ID:SGTC_2932|Compound:9016711|FD-Score:2.82|P-value:6.55E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.24|P-value:8.82E-5 ID:SGTC_2958|Compound:9077254|FD-Score:3.85|P-value:5.56E-6 ID:SGTC_2996|Compound:9066192|FD-Score:4.00|P-value:2.50E-6 ID:SGTC_3030|Compound:9087619|FD-Score:2.84|P-value:6.04E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.28|P-value:3.77E-7 ID:SGTC_3195|Compound:9113082|FD-Score:2.83|P-value:6.30E-4 ID:SGTC_3289|Compound:9119912|FD-Score:3.43|P-value:4.61E-5 ID:SGTC_3324|Compound:9138759|FD-Score:2.91|P-value:4.66E-4

Top fitness defect scores for YOL013C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 10.60 100.0 µM 1.48E-34 redox potentiating 55 9.60E-27
2 SGTC_513 4245-1575 7.10 41.7 µM 2.11E-16 unfolded protein response 33 6.28E-13
3 SGTC_1912 st060209 6.98 90.8 µM 6.49E-16 4 1.48E-12
4 SGTC_1916 st067606 6.40 49.2 µM 1.18E-13 7 7.84E-11
5 SGTC_2324 6149169 5.94 200.0 µM 5.39E-12 3 1.45E-9
6 SGTC_2584 androsterone 5.32 48.7 µM 5.71E-10 cell wall 72 5.15E-8
7 SGTC_2261 9010538 5.12 184.7 µM 2.40E-9 4 1.55E-7
8 SGTC_1072 oxiconazole 4.54 14.9 nM 1.08E-7 ergosterol depletion effects on membrane 39 2.87E-6
9 SGTC_2467 5474540 4.51 200.0 µM 1.27E-7 8 3.24E-6
10 SGTC_865 0488-0499 4.41 301.0 µM 2.37E-7 11 5.25E-6
11 SGTC_16 erodoxin 4.40 22.8 µM 2.41E-7 unfolded protein response 50 5.32E-6
12 SGTC_2996 9066192 4.00 71.4 µM 2.50E-6 30 3.22E-5
13 SGTC_1161 k007-0610 3.96 110.0 µM 3.10E-6 ERAD & cell cycle 120 3.79E-5
14 SGTC_2958 9077254 3.85 16.7 µM 5.56E-6 34 5.96E-5
15 SGTC_2572 astragaloside iv 3.76 100.0 µM 8.96E-6 35 8.61E-5
16 SGTC_252 miconazole 3.68 1.3 nM 1.34E-5 ergosterol depletion effects on membrane 40 1.17E-4
17 SGTC_588 3970-1144 3.58 52.9 µM 2.17E-5 35 1.70E-4
18 SGTC_3289 9119912 3.43 71.4 µM 4.61E-5 29 3.05E-4
19 SGTC_1044 1222-0021 3.38 1.8 µM 5.77E-5 heme biosynthesis & mitochondrial translocase 58 3.63E-4
20 SGTC_1893 st059353 3.36 36.1 µM 6.30E-5 47 3.89E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.325 4.47E-83 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.267 5.97E-56 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.232 4.09E-42 YLR238W FAR10 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
0.222 7.18E-39 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.219 9.18E-38 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.211 3.43E-35 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.208 3.49E-34 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.205 3.06E-33 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.204 1.01E-32 YJR091C JSN1 hom Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl
0.202 2.41E-32 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.202 3.55E-32 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.200 1.08E-31 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.198 5.01E-31 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.194 1.11E-29 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.190 2.03E-28 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress