YOL016C / CMK2

Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL016C / CMK2

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:4.27|P-value:9.84E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:6.29|P-value:1.47E-10 ID:SGTC_92|Compound:0063-0024|FD-Score:3.44|P-value:3.03E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:3.17|P-value:7.93E-4 ID:SGTC_229|Compound:benomyl|FD-Score:4.35|P-value:6.89E-6 ID:SGTC_360|Compound:0957-0399|FD-Score:3.77|P-value:8.42E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.14|P-value:7.06E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:6.58|P-value:2.22E-11 ID:SGTC_467|Compound:ro 31-8220|FD-Score:14.80|P-value:1.41E-50 ID:SGTC_502|Compound:l-744,832|FD-Score:3.34|P-value:4.27E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:7.82|P-value:2.22E-15 ID:SGTC_582|Compound:0987-0078|FD-Score:8.14|P-value:1.67E-16 ID:SGTC_592|Compound:k241-0073|FD-Score:4.12|P-value:1.92E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:3.26|P-value:5.72E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.30|P-value:5.07E-4 ID:SGTC_678|Compound:1218-2179|FD-Score:3.70|P-value:1.12E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.14|P-value:8.67E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:3.82|P-value:6.98E-5 ID:SGTC_729|Compound:4092-0585|FD-Score:4.60|P-value:2.14E-6 ID:SGTC_892|Compound:0929-0076|FD-Score:3.28|P-value:5.30E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:5.25|P-value:7.38E-8 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.72|P-value:1.18E-6 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.47|P-value:2.67E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.18|P-value:7.63E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.68|P-value:1.45E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.41|P-value:5.33E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:8.95|P-value:1.35E-19 ID:SGTC_1672|Compound:st016598|FD-Score:-3.14|P-value:7.18E-4 ID:SGTC_1708|Compound:st033234|FD-Score:5.35|P-value:4.46E-8 ID:SGTC_1712|Compound:st032288|FD-Score:11.90|P-value:4.92E-33 ID:SGTC_1728|Compound:st035614|FD-Score:4.38|P-value:5.94E-6 ID:SGTC_1735|Compound:st034310|FD-Score:-4.25|P-value:8.17E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.16|P-value:8.19E-4 ID:SGTC_1826|Compound:st055655|FD-Score:4.71|P-value:1.28E-6 ID:SGTC_1987|Compound:st072290|FD-Score:3.35|P-value:4.15E-4 ID:SGTC_2253|Compound:7938677|FD-Score:3.58|P-value:1.77E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.10|P-value:2.16E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.59|P-value:2.20E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-4.05|P-value:1.98E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.57|P-value:2.42E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.83|P-value:6.57E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.89|P-value:1.87E-9 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.18|P-value:6.12E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.17|P-value:8.02E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.21|P-value:2.58E-13 ID:SGTC_2942|Compound:9052777|FD-Score:3.37|P-value:3.95E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.27|P-value:5.50E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.19|P-value:6.02E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.70|P-value:1.11E-4 ID:SGTC_3324|Compound:9138759|FD-Score:3.67|P-value:1.25E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:4.27|P-value:9.84E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:6.29|P-value:1.47E-10 ID:SGTC_92|Compound:0063-0024|FD-Score:3.44|P-value:3.03E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:3.17|P-value:7.93E-4 ID:SGTC_229|Compound:benomyl|FD-Score:4.35|P-value:6.89E-6 ID:SGTC_360|Compound:0957-0399|FD-Score:3.77|P-value:8.42E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.14|P-value:7.06E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:6.58|P-value:2.22E-11 ID:SGTC_467|Compound:ro 31-8220|FD-Score:14.80|P-value:1.41E-50 ID:SGTC_502|Compound:l-744,832|FD-Score:3.34|P-value:4.27E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:7.82|P-value:2.22E-15 ID:SGTC_582|Compound:0987-0078|FD-Score:8.14|P-value:1.67E-16 ID:SGTC_592|Compound:k241-0073|FD-Score:4.12|P-value:1.92E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:3.26|P-value:5.72E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.30|P-value:5.07E-4 ID:SGTC_678|Compound:1218-2179|FD-Score:3.70|P-value:1.12E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.14|P-value:8.67E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:3.82|P-value:6.98E-5 ID:SGTC_729|Compound:4092-0585|FD-Score:4.60|P-value:2.14E-6 ID:SGTC_892|Compound:0929-0076|FD-Score:3.28|P-value:5.30E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:5.25|P-value:7.38E-8 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.72|P-value:1.18E-6 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.47|P-value:2.67E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.18|P-value:7.63E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.68|P-value:1.45E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.41|P-value:5.33E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:8.95|P-value:1.35E-19 ID:SGTC_1672|Compound:st016598|FD-Score:-3.14|P-value:7.18E-4 ID:SGTC_1708|Compound:st033234|FD-Score:5.35|P-value:4.46E-8 ID:SGTC_1712|Compound:st032288|FD-Score:11.90|P-value:4.92E-33 ID:SGTC_1728|Compound:st035614|FD-Score:4.38|P-value:5.94E-6 ID:SGTC_1735|Compound:st034310|FD-Score:-4.25|P-value:8.17E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.16|P-value:8.19E-4 ID:SGTC_1826|Compound:st055655|FD-Score:4.71|P-value:1.28E-6 ID:SGTC_1987|Compound:st072290|FD-Score:3.35|P-value:4.15E-4 ID:SGTC_2253|Compound:7938677|FD-Score:3.58|P-value:1.77E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.10|P-value:2.16E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.59|P-value:2.20E-6 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-4.05|P-value:1.98E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.57|P-value:2.42E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.83|P-value:6.57E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.89|P-value:1.87E-9 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.18|P-value:6.12E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.17|P-value:8.02E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.21|P-value:2.58E-13 ID:SGTC_2942|Compound:9052777|FD-Score:3.37|P-value:3.95E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.27|P-value:5.50E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.19|P-value:6.02E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.70|P-value:1.11E-4 ID:SGTC_3324|Compound:9138759|FD-Score:3.67|P-value:1.25E-4

Top fitness defect scores for YOL016C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_467 ro 31-8220 14.80 72.8 µM 1.41E-50 calcium & mitochondrial duress 2 3.69E-50
2 SGTC_1712 st032288 11.90 53.4 µM 4.92E-33 calcium & mitochondrial duress 11 8.42E-33
3 SGTC_1597 7-benzyloxygramine 8.95 47.5 µM 1.35E-19 calcium & mitochondrial duress 19 1.71E-19
4 SGTC_582 0987-0078 8.14 156.0 µM 1.67E-16 calcium & mitochondrial duress 12 1.98E-16
5 SGTC_528 3492-0019 7.82 172.0 µM 2.22E-15 calcium & mitochondrial duress 77 2.58E-15
6 SGTC_2896 9052656 7.21 71.4 µM 2.58E-13 endomembrane recycling 25 2.88E-13
7 SGTC_439 amiodarone 6.58 34.4 µM 2.22E-11 NEO1-PIK1 38 2.39E-11
8 SGTC_65 0798-0905 6.29 35.0 µM 1.47E-10 calcium & mitochondrial duress 24 1.56E-10
9 SGTC_2688 kanamycin b 5.89 96.7 µM 1.87E-9 calcium & mitochondrial duress 163 1.95E-9
10 SGTC_1708 st033234 5.35 31.8 µM 4.46E-8 4 4.52E-8
11 SGTC_913 3487-1245 5.25 167.0 µM 7.38E-8 5 7.46E-8
12 SGTC_1043 0916-0009 4.72 19.3 µM 1.18E-6 10 1.17E-6
13 SGTC_1826 st055655 4.71 41.6 µM 1.28E-6 ERG2 28 1.27E-6
14 SGTC_1256 0570-0116 4.68 285.0 µM 1.45E-6 7 1.44E-6
15 SGTC_729 4092-0585 4.60 94.1 µM 2.14E-6 14 2.12E-6
16 SGTC_2482 5981709 4.59 142.7 µM 2.20E-6 PDR1 28 2.17E-6
17 SGTC_2619 obliquin 4.57 87.9 µM 2.42E-6 cell wall 113 2.40E-6
18 SGTC_1310 1188-0297 4.41 994.0 nM 5.33E-6 azole & statin 22 5.24E-6
19 SGTC_1728 st035614 4.38 33.8 µM 5.94E-6 32 5.84E-6
20 SGTC_229 benomyl 4.35 22.9 µM 6.89E-6 tubulin folding & SWR complex 24 6.77E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.495 1.78E-206 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.439 5.78E-158 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.384 3.29E-118 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.361 3.61E-104 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.355 1.57E-100 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.339 5.37E-91 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.309 3.30E-75 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.286 4.33E-64 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.259 9.47E-53 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.256 2.58E-51 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.253 3.60E-50 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.240 2.68E-45 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.239 6.99E-45 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.236 1.33E-43 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.234 3.65E-43 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS