YOL032W / OPI10

Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL032W / OPI10

Click on Significant Values for Screen Details ID:SGTC_159|Compound:3970-0959|FD-Score:3.65|P-value:7.07E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.29|P-value:5.80E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.23|P-value:7.17E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.68|P-value:6.23E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.01|P-value:7.88E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.32|P-value:5.20E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.14|P-value:5.01E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.57|P-value:9.76E-5 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.38|P-value:2.02E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.16|P-value:9.11E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.94|P-value:2.03E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.98|P-value:8.77E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.09|P-value:6.03E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.08|P-value:6.16E-4 ID:SGTC_1762|Compound:st045503|FD-Score:3.52|P-value:1.19E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.70|P-value:1.19E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.89|P-value:2.57E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.06|P-value:6.67E-4 ID:SGTC_2197|Compound:6588183|FD-Score:-3.29|P-value:5.71E-4 ID:SGTC_2203|Compound:7227154|FD-Score:-4.33|P-value:8.04E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-3.67|P-value:1.38E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.30|P-value:3.94E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:5.98|P-value:3.14E-10 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.71|P-value:2.18E-12 ID:SGTC_2798|Compound:7813637|FD-Score:4.84|P-value:2.66E-7 ID:SGTC_3208|Compound:9114115|FD-Score:4.71|P-value:5.02E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:3.65|P-value:7.07E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.29|P-value:5.80E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.23|P-value:7.17E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.68|P-value:6.23E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.01|P-value:7.88E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.32|P-value:5.20E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.14|P-value:5.01E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.57|P-value:9.76E-5 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.38|P-value:2.02E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.16|P-value:9.11E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.94|P-value:2.03E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.98|P-value:8.77E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.09|P-value:6.03E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.08|P-value:6.16E-4 ID:SGTC_1762|Compound:st045503|FD-Score:3.52|P-value:1.19E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.70|P-value:1.19E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.89|P-value:2.57E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.06|P-value:6.67E-4 ID:SGTC_2197|Compound:6588183|FD-Score:-3.29|P-value:5.71E-4 ID:SGTC_2203|Compound:7227154|FD-Score:-4.33|P-value:8.04E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-3.67|P-value:1.38E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.30|P-value:3.94E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:5.98|P-value:3.14E-10 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.71|P-value:2.18E-12 ID:SGTC_2798|Compound:7813637|FD-Score:4.84|P-value:2.66E-7 ID:SGTC_3208|Compound:9114115|FD-Score:4.71|P-value:5.02E-7

Top fitness defect scores for YOL032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2638 epoxomicin 6.71 18.0 µM 2.18E-12 3 9.50E-12
2 SGTC_2574 2',4'-dihydroxychalcone 5.98 14.6 µM 3.14E-10 6 1.09E-9
3 SGTC_2798 7813637 4.84 45.5 µM 2.66E-7 14 6.64E-7
4 SGTC_3208 9114115 4.71 49.5 µM 5.02E-7 21 1.21E-6
5 SGTC_1417 3966-0327 4.38 90.4 µM 2.70E-6 10 5.99E-6
6 SGTC_2434 5670830 4.30 169.9 µM 3.94E-6 18 8.59E-6
7 SGTC_1570 verbenone 3.94 133.0 µM 2.03E-5 16 4.06E-5
8 SGTC_1796 st049173 3.89 110.4 µM 2.57E-5 16 5.07E-5
9 SGTC_334 1502-1024 3.68 68.5 µM 6.23E-5 17 1.17E-4
10 SGTC_159 3970-0959 3.65 50.0 µM 7.07E-5 97 1.32E-4
11 SGTC_1127 asiatic acid 3.57 681.4 nM 9.76E-5 36 1.80E-4
12 SGTC_1762 st045503 3.52 25.5 µM 1.19E-4 39 2.16E-4
13 SGTC_1280 0880-0253 3.38 10.1 µM 2.02E-4 33 3.58E-4
14 SGTC_967 1306-0166 3.14 352.0 µM 5.01E-4 72 8.43E-4
15 SGTC_1630 st005209 3.09 31.3 µM 6.03E-4 64 0.00100
16 SGTC_1699 st031318 3.08 29.1 µM 6.16E-4 52 0.00102
17 SGTC_1820 st053765 3.06 18.6 µM 6.67E-4 RPP1 & pyrimidine depletion 61 0.00110
18 SGTC_362 0310-0100 3.01 9.0 µM 7.88E-4 RPP1 & pyrimidine depletion 82 0.00129
19 SGTC_1191 1487-1166 3.00 10.6 µM 8.16E-4 azole & statin 72 0.00134
20 SGTC_1576 erythromycin ethylsuccinate 2.98 23.2 µM 8.77E-4 65 0.00143

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 1.01E-16 YMR130W_p YMR130W_p hom Putative protein of unknown function; YMR130W is not an essential gene
0.133 9.52E-15 YOL122C SMF1 hom Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
0.126 2.42E-13 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.118 6.09E-12 YDL229W SSB1 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
0.109 2.53E-10 YER101C AST2 hom Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
0.104 1.68E-9 YMR028W TAP42 het Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
0.102 2.84E-9 YDR399W HPT1 hom Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
0.102 3.46E-9 YPR201W ARR3 hom Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
0.100 6.84E-9 YDL164C CDC9 het DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
0.100 7.46E-9 YPR154W PIN3 hom Protein that induces appearance of [PIN+] prion when overproduced
0.098 1.11E-8 YGR209C TRX2 hom Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.095 3.01E-8 YPR085C ASA1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.091 1.45E-7 YGR068C ART5 hom Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane
0.089 2.13E-7 YJR025C BNA1 hom 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
0.088 3.81E-7 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains