YOL043C / NTG2

DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL043C / NTG2

Click on Significant Values for Screen Details ID:SGTC_401|Compound:neomycin|FD-Score:-3.36|P-value:9.08E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:3.03|P-value:3.25E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-2.91|P-value:5.96E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:2.74|P-value:9.92E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:2.80|P-value:8.03E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.01|P-value:4.04E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.84|P-value:8.91E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:3.07|P-value:2.71E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.32|P-value:9.10E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:2.86|P-value:6.25E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.52|P-value:1.87E-7 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.15|P-value:1.97E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.07|P-value:2.74E-4 ID:SGTC_1612|Compound:st002233|FD-Score:2.75|P-value:9.80E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.45|P-value:2.73E-7 ID:SGTC_1636|Compound:st007707|FD-Score:2.88|P-value:5.74E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.06|P-value:2.83E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.68|P-value:7.23E-8 ID:SGTC_1813|Compound:st051246|FD-Score:3.59|P-value:2.65E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.21|P-value:1.52E-4 ID:SGTC_1923|Compound:st058458|FD-Score:2.95|P-value:4.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:2.85|P-value:6.53E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-2.79|P-value:9.39E-4 ID:SGTC_2262|Compound:9014885|FD-Score:2.79|P-value:8.27E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.05|P-value:2.91E-4 ID:SGTC_2374|Compound:9071832|FD-Score:3.45|P-value:5.19E-5 ID:SGTC_2382|Compound:9035899|FD-Score:-2.84|P-value:7.64E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-5.08|P-value:6.81E-9 ID:SGTC_2388|Compound:9080168|FD-Score:-4.46|P-value:3.16E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.14|P-value:1.99E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.41|P-value:6.10E-5 ID:SGTC_2797|Compound:7859098|FD-Score:3.67|P-value:1.84E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.38|P-value:7.14E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-3.36|P-value:9.08E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:3.03|P-value:3.25E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-2.91|P-value:5.96E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:2.74|P-value:9.92E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:2.80|P-value:8.03E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.01|P-value:4.04E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.84|P-value:8.91E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:3.07|P-value:2.71E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.32|P-value:9.10E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:2.86|P-value:6.25E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.52|P-value:1.87E-7 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.15|P-value:1.97E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.07|P-value:2.74E-4 ID:SGTC_1612|Compound:st002233|FD-Score:2.75|P-value:9.80E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.45|P-value:2.73E-7 ID:SGTC_1636|Compound:st007707|FD-Score:2.88|P-value:5.74E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-3.06|P-value:3.28E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.06|P-value:2.83E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.68|P-value:7.23E-8 ID:SGTC_1813|Compound:st051246|FD-Score:3.59|P-value:2.65E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.21|P-value:1.52E-4 ID:SGTC_1923|Compound:st058458|FD-Score:2.95|P-value:4.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:2.85|P-value:6.53E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-2.79|P-value:9.39E-4 ID:SGTC_2262|Compound:9014885|FD-Score:2.79|P-value:8.27E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.05|P-value:2.91E-4 ID:SGTC_2374|Compound:9071832|FD-Score:3.45|P-value:5.19E-5 ID:SGTC_2382|Compound:9035899|FD-Score:-2.84|P-value:7.64E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-5.08|P-value:6.81E-9 ID:SGTC_2388|Compound:9080168|FD-Score:-4.46|P-value:3.16E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.14|P-value:1.99E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.41|P-value:6.10E-5 ID:SGTC_2797|Compound:7859098|FD-Score:3.67|P-value:1.84E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.38|P-value:7.14E-5

Top fitness defect scores for YOL043C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1808 st050063 4.68 61.5 µM 7.23E-8 4 1.46E-6
2 SGTC_1400 3771-8998 4.52 51.5 µM 1.87E-7 3 3.12E-6
3 SGTC_1619 st003326 4.45 88.7 µM 2.73E-7 60S ribosome export 11 4.23E-6
4 SGTC_2797 7859098 3.67 81.8 µM 1.84E-5 35 1.23E-4
5 SGTC_1813 st051246 3.59 8.6 µM 2.65E-5 TSC3-RPN4 16 1.65E-4
6 SGTC_2374 9071832 3.45 200.0 µM 5.19E-5 endomembrane recycling 17 2.84E-4
7 SGTC_2678 equilin 3.41 100.0 µM 6.10E-5 37 3.23E-4
8 SGTC_3225 9130819 3.38 49.5 µM 7.14E-5 amide catabolism 45 3.66E-4
9 SGTC_1271 0832-2808 3.32 169.0 µM 9.10E-5 26 4.45E-4
10 SGTC_1830 st055982 3.21 50.3 µM 1.52E-4 54 6.73E-4
11 SGTC_973 1325-0077 3.19 65.9 µM 1.65E-4 32 7.18E-4
12 SGTC_1445 4239-0156 3.15 97.7 µM 1.97E-4 67 8.28E-4
13 SGTC_2588 indole-3-carbinol 3.14 100.0 µM 1.99E-4 94 8.35E-4
14 SGTC_994 3910-0338 3.07 138.0 µM 2.71E-4 calcium & mitochondrial duress 92 0.00107
15 SGTC_1449 4112-4002 3.07 201.0 µM 2.74E-4 RPP1 & pyrimidine depletion 42 0.00108
16 SGTC_1714 st033236 3.06 13.6 µM 2.83E-4 64 0.00111
17 SGTC_2369 9071472 3.05 200.0 µM 2.91E-4 50 0.00114
18 SGTC_426 0988-0037 3.03 14.6 µM 3.25E-4 ERAD & cell cycle 82 0.00124
19 SGTC_1923 st058458 2.95 19.1 µM 4.36E-4 96 0.00157
20 SGTC_1636 st007707 2.88 68.9 µM 5.74E-4 111 0.00196

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 1.06E-14 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.131 2.47E-14 YDR022C ATG31 hom Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion
0.113 4.97E-11 YJL125C GCD14 het Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
0.112 8.89E-11 YNL254C RTC4 hom Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.093 5.95E-8 YDL219W DTD1 hom D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
0.093 7.45E-8 YGR182C_d YGR182C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W
0.088 3.80E-7 YKL059C MPE1 het Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
0.085 8.43E-7 YPR112C MRD1 het Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
0.083 1.52E-6 YBR153W RIB7 het Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
0.082 1.81E-6 YDL241W_p YDL241W_p hom Putative protein of unknown function; YDL241W is not an essential gene
0.081 2.39E-6 YMR235C RNA1 het GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
0.081 2.46E-6 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.079 4.13E-6 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.078 5.69E-6 YJL200C ACO2 hom Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
0.077 8.52E-6 YBR108W AIM3 hom Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss