YOL055C / THI20

Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL055C / THI20

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.32|P-value:1.35E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:3.76|P-value:1.92E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-5.23|P-value:4.60E-9 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.95|P-value:2.70E-8 ID:SGTC_263|Compound:alizarin|FD-Score:3.72|P-value:2.32E-5 ID:SGTC_497|Compound:capsazepine|FD-Score:4.90|P-value:3.85E-8 ID:SGTC_621|Compound:3584-0052|FD-Score:4.13|P-value:2.96E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:3.02|P-value:4.59E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.70|P-value:2.52E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.03|P-value:4.45E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.06|P-value:3.97E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-2.81|P-value:9.98E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:2.90|P-value:7.54E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.06|P-value:3.94E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.22|P-value:2.11E-4 ID:SGTC_1741|Compound:st038134|FD-Score:3.26|P-value:1.79E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.08|P-value:3.66E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.71|P-value:1.25E-7 ID:SGTC_2307|Compound:7538926|FD-Score:3.37|P-value:1.10E-4 ID:SGTC_2308|Compound:7584454|FD-Score:2.85|P-value:8.92E-4 ID:SGTC_2309|Compound:7664088|FD-Score:4.03|P-value:5.13E-6 ID:SGTC_2477|Compound:5967686|FD-Score:-2.84|P-value:9.18E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.46|P-value:5.10E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.35|P-value:1.20E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-6.04|P-value:1.68E-11 ID:SGTC_2714|Compound:st079313|FD-Score:-2.88|P-value:7.77E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.55|P-value:3.14E-7 ID:SGTC_2850|Compound:9015429|FD-Score:2.92|P-value:6.84E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-2.93|P-value:6.48E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.06|P-value:3.96E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.24|P-value:1.96E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-5.56|P-value:5.11E-10 ID:SGTC_2958|Compound:9077254|FD-Score:3.63|P-value:3.52E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.20|P-value:2.24E-4 ID:SGTC_3091|Compound:9116372|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.84|P-value:9.19E-4 ID:SGTC_3252|Compound:9135871|FD-Score:2.94|P-value:6.44E-4 ID:SGTC_3299|Compound:9121982|FD-Score:2.86|P-value:8.66E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.32|P-value:1.35E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:3.76|P-value:1.92E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-5.23|P-value:4.60E-9 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.95|P-value:2.70E-8 ID:SGTC_263|Compound:alizarin|FD-Score:3.72|P-value:2.32E-5 ID:SGTC_497|Compound:capsazepine|FD-Score:4.90|P-value:3.85E-8 ID:SGTC_621|Compound:3584-0052|FD-Score:4.13|P-value:2.96E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:3.02|P-value:4.59E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.70|P-value:2.52E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.03|P-value:4.45E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.06|P-value:3.97E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-2.81|P-value:9.98E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:2.90|P-value:7.54E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.06|P-value:3.94E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.22|P-value:2.11E-4 ID:SGTC_1741|Compound:st038134|FD-Score:3.26|P-value:1.79E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.08|P-value:3.66E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.71|P-value:1.25E-7 ID:SGTC_2307|Compound:7538926|FD-Score:3.37|P-value:1.10E-4 ID:SGTC_2308|Compound:7584454|FD-Score:2.85|P-value:8.92E-4 ID:SGTC_2309|Compound:7664088|FD-Score:4.03|P-value:5.13E-6 ID:SGTC_2477|Compound:5967686|FD-Score:-2.84|P-value:9.18E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.46|P-value:5.10E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.35|P-value:1.20E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-6.04|P-value:1.68E-11 ID:SGTC_2714|Compound:st079313|FD-Score:-2.88|P-value:7.77E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.55|P-value:3.14E-7 ID:SGTC_2850|Compound:9015429|FD-Score:2.92|P-value:6.84E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-2.93|P-value:6.48E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.06|P-value:3.96E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.24|P-value:1.96E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-5.56|P-value:5.11E-10 ID:SGTC_2958|Compound:9077254|FD-Score:3.63|P-value:3.52E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.20|P-value:2.24E-4 ID:SGTC_3091|Compound:9116372|FD-Score:3.36|P-value:1.17E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.84|P-value:9.19E-4 ID:SGTC_3252|Compound:9135871|FD-Score:2.94|P-value:6.44E-4 ID:SGTC_3299|Compound:9121982|FD-Score:2.86|P-value:8.66E-4

Top fitness defect scores for YOL055C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_497 capsazepine 4.90 133.0 µM 3.85E-8 2 4.74E-7
2 SGTC_2012 4023501 4.71 200.0 µM 1.25E-7 17 1.27E-6
3 SGTC_2848 9016370 4.55 26.0 µM 3.14E-7 12 2.74E-6
4 SGTC_2481 5478948 4.46 45.2 µM 5.10E-7 PDR1 24 4.11E-6
5 SGTC_621 3584-0052 4.13 32.1 µM 2.96E-6 24 1.80E-5
6 SGTC_2309 7664088 4.03 163.9 µM 5.13E-6 24 2.85E-5
7 SGTC_111 0083-0039 3.76 52.5 µM 1.92E-5 4 8.60E-5
8 SGTC_263 alizarin 3.72 107.9 µM 2.32E-5 iron homeostasis 66 1.01E-4
9 SGTC_746 1095-0025 3.70 46.6 µM 2.52E-5 RSC & ERG11 19 1.08E-4
10 SGTC_2958 9077254 3.63 16.7 µM 3.52E-5 40 1.43E-4
11 SGTC_2307 7538926 3.37 6.3 µM 1.10E-4 60 3.73E-4
12 SGTC_3091 9116372 3.36 49.5 µM 1.17E-4 30 3.93E-4
13 SGTC_2636 apigenin dimethyl ether 3.35 100.0 µM 1.20E-4 72 4.02E-4
14 SGTC_36 0335-0881 3.32 14.2 µM 1.35E-4 ubiquinone biosynthesis & proteasome 154 4.43E-4
15 SGTC_1741 st038134 3.26 10.2 µM 1.79E-4 60 5.63E-4
16 SGTC_2916 7875993 3.24 68.8 µM 1.96E-4 55 6.07E-4
17 SGTC_1420 4'-methoxyflavone 3.22 42.0 µM 2.11E-4 31 6.45E-4
18 SGTC_3063 1327-0034 3.20 4.0 µM 2.24E-4 63 6.80E-4
19 SGTC_1743 st037606 3.08 46.4 µM 3.66E-4 65 0.00103
20 SGTC_2913 7966556 3.06 36.6 µM 3.96E-4 60S ribosome export 46 0.00110

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.31E-27 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.179 1.86E-25 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.154 3.98E-19 YGL249W ZIP2 hom Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
0.115 2.66E-11 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.111 1.09E-10 YBL035C POL12 het B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
0.106 8.78E-10 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
0.104 1.80E-9 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.099 8.21E-9 YDR356W SPC110 het Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
0.095 3.26E-8 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.095 4.03E-8 YOR226C ISU2 hom Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved
0.093 6.66E-8 YDR139C RUB1 hom Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
0.091 1.42E-7 YPL133C RDS2 hom Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
0.089 2.62E-7 YFR046C CNN1 hom Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
0.087 4.41E-7 YIL087C AIM19 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth
0.087 4.84E-7 YOL125W TRM13 hom 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases