HIPHOP chemogenomics database

YOL059W / GPD2

NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1

Zygosity: Homozygous strain

fixedexpanded
Profile for YOL059W / GPD2

Click on Significant Values for Screen Details

Top fitness defect scores for YOL059W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2758	dehydroepiandrosterone	7.54	69.3 µM	9.40E-12		16	2.42E-14
2	SGTC_574	1315-0397	5.92	40.0 µM	7.08E-8		6	1.65E-9
3	SGTC_1038	k292-0204	5.79	154.0 µM	1.29E-7		3	3.49E-9
4	SGTC_2989	9073649	5.14	71.4 µM	2.48E-6		13	1.39E-7
5	SGTC_1761	st044296	4.95	37.0 µM	5.35E-6		5	3.62E-7
6	SGTC_3223	9129970	4.91	49.5 µM	6.56E-6		10	4.67E-7
7	SGTC_2593	nobiletin	4.69	86.2 µM	1.53E-5		14	1.34E-6
8	SGTC_410	α-linolenic acid	4.62	100.0 µM	2.00E-5	excess fatty acid	21	1.88E-6
9	SGTC_573	3702-0553	4.41	84.7 µM	4.49E-5		8	5.12E-6
10	SGTC_860	0443-0016	4.40	25.1 µM	4.69E-5		22	5.40E-6
11	SGTC_2284	7945075	4.37	39.2 µM	5.27E-5	mitochondrial processes	18	6.24E-6
12	SGTC_1984	st072354	4.36	53.1 µM	5.51E-5		8	6.60E-6
13	SGTC_1357	1503-1303	4.24	39.5 µM	8.47E-5		23	1.13E-5
14	SGTC_121	0557-0495	4.17	61.3 µM	1.07E-4	calcium & mitochondrial duress	49	1.51E-5
15	SGTC_1265	0819-1001	4.17	68.8 µM	1.08E-4		21	1.52E-5
16	SGTC_1263	0774-1208	3.92	64.8 µM	2.57E-4		50	4.48E-5
17	SGTC_528	3492-0019	3.84	172.0 µM	3.27E-4	calcium & mitochondrial duress	231	6.04E-5
18	SGTC_2823	7987624	3.71	58.4 µM	4.97E-4		38	1.02E-4
19	SGTC_2026	5148700	3.56	152.0 µM	8.00E-4		28	1.83E-4
20	SGTC_114	0364-0001	3.54	490.3 µM	8.61E-4		67	2.01E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.172	1.20E-23	YGL015C_p	YGL015C_p	hom	Putative protein of unknown function; null mutants accumulate cargo in the Golgi
0.169	6.39E-23	YDR475C	JIP4	hom	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.122	1.62E-12	YOR086C	TCB1	hom	Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.116	1.79E-11	YGR237C_p	YGR237C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.116	1.95E-11	YJL165C	HAL5	hom	Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.111	1.09E-10	YPR050C_d	YPR050C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.107	4.47E-10	YKL051W	SFK1	hom	Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.106	7.06E-10	YJL129C	TRK1	hom	Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.101	4.05E-9	YCR008W	SAT4	hom	Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
0.097	2.11E-8	YEL008W_d	YEL008W_d	hom	Hypothetical protein predicted to be involved in metabolism
0.096	2.64E-8	YBL051C	PIN4	hom	Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.096	2.93E-8	YLL047W_d	YLL047W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
0.095	3.64E-8	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.095	4.12E-8	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.094	5.24E-8	YOL081W	IRA2	hom	GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication