YOL059W / GPD2

NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL059W / GPD2

Click on Significant Values for Screen Details ID:SGTC_114|Compound:0364-0001|FD-Score:3.54|P-value:8.61E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.17|P-value:1.07E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.80|P-value:2.93E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.62|P-value:2.00E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.84|P-value:3.27E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.41|P-value:4.49E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:5.92|P-value:7.08E-8 ID:SGTC_860|Compound:0443-0016|FD-Score:4.40|P-value:4.69E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.82|P-value:2.72E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:5.79|P-value:1.29E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.43|P-value:9.26E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.92|P-value:2.57E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:4.17|P-value:1.08E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.24|P-value:8.47E-5 ID:SGTC_1761|Compound:st044296|FD-Score:4.95|P-value:5.35E-6 ID:SGTC_1984|Compound:st072354|FD-Score:4.36|P-value:5.51E-5 ID:SGTC_1989|Compound:st071868|FD-Score:-3.47|P-value:8.31E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-4.04|P-value:1.27E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.56|P-value:8.00E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.92|P-value:1.93E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.37|P-value:5.27E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.82|P-value:6.81E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.69|P-value:1.53E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:7.54|P-value:9.40E-12 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.45|P-value:8.72E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.71|P-value:4.97E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.14|P-value:2.48E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-3.60|P-value:5.49E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-4.08|P-value:1.12E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.91|P-value:6.56E-6 ID:SGTC_114|Compound:0364-0001|FD-Score:3.54|P-value:8.61E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.17|P-value:1.07E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.80|P-value:2.93E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.62|P-value:2.00E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.84|P-value:3.27E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.41|P-value:4.49E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:5.92|P-value:7.08E-8 ID:SGTC_860|Compound:0443-0016|FD-Score:4.40|P-value:4.69E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.82|P-value:2.72E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:5.79|P-value:1.29E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.43|P-value:9.26E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.92|P-value:2.57E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:4.17|P-value:1.08E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.24|P-value:8.47E-5 ID:SGTC_1761|Compound:st044296|FD-Score:4.95|P-value:5.35E-6 ID:SGTC_1984|Compound:st072354|FD-Score:4.36|P-value:5.51E-5 ID:SGTC_1989|Compound:st071868|FD-Score:-3.47|P-value:8.31E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-4.04|P-value:1.27E-4 ID:SGTC_2026|Compound:5148700|FD-Score:3.56|P-value:8.00E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.92|P-value:1.93E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.37|P-value:5.27E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.82|P-value:6.81E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.69|P-value:1.53E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:7.54|P-value:9.40E-12 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.45|P-value:8.72E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.71|P-value:4.97E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.14|P-value:2.48E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-3.60|P-value:5.49E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-4.08|P-value:1.12E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.91|P-value:6.56E-6

Top fitness defect scores for YOL059W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2758 dehydroepiandrosterone 7.54 69.3 µM 9.40E-12 16 2.42E-14
2 SGTC_574 1315-0397 5.92 40.0 µM 7.08E-8 6 1.65E-9
3 SGTC_1038 k292-0204 5.79 154.0 µM 1.29E-7 3 3.49E-9
4 SGTC_2989 9073649 5.14 71.4 µM 2.48E-6 13 1.39E-7
5 SGTC_1761 st044296 4.95 37.0 µM 5.35E-6 5 3.62E-7
6 SGTC_3223 9129970 4.91 49.5 µM 6.56E-6 10 4.67E-7
7 SGTC_2593 nobiletin 4.69 86.2 µM 1.53E-5 14 1.34E-6
8 SGTC_410 α-linolenic acid 4.62 100.0 µM 2.00E-5 excess fatty acid 21 1.88E-6
9 SGTC_573 3702-0553 4.41 84.7 µM 4.49E-5 8 5.12E-6
10 SGTC_860 0443-0016 4.40 25.1 µM 4.69E-5 22 5.40E-6
11 SGTC_2284 7945075 4.37 39.2 µM 5.27E-5 mitochondrial processes 18 6.24E-6
12 SGTC_1984 st072354 4.36 53.1 µM 5.51E-5 8 6.60E-6
13 SGTC_1357 1503-1303 4.24 39.5 µM 8.47E-5 23 1.13E-5
14 SGTC_121 0557-0495 4.17 61.3 µM 1.07E-4 calcium & mitochondrial duress 49 1.51E-5
15 SGTC_1265 0819-1001 4.17 68.8 µM 1.08E-4 21 1.52E-5
16 SGTC_1263 0774-1208 3.92 64.8 µM 2.57E-4 50 4.48E-5
17 SGTC_528 3492-0019 3.84 172.0 µM 3.27E-4 calcium & mitochondrial duress 231 6.04E-5
18 SGTC_2823 7987624 3.71 58.4 µM 4.97E-4 38 1.02E-4
19 SGTC_2026 5148700 3.56 152.0 µM 8.00E-4 28 1.83E-4
20 SGTC_114 0364-0001 3.54 490.3 µM 8.61E-4 67 2.01E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 1.20E-23 YGL015C_p YGL015C_p hom Putative protein of unknown function; null mutants accumulate cargo in the Golgi
0.169 6.39E-23 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.122 1.62E-12 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.116 1.79E-11 YGR237C_p YGR237C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.116 1.95E-11 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.111 1.09E-10 YPR050C_d YPR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.107 4.47E-10 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.106 7.06E-10 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.101 4.05E-9 YCR008W SAT4 hom Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
0.097 2.11E-8 YEL008W_d YEL008W_d hom Hypothetical protein predicted to be involved in metabolism
0.096 2.64E-8 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.096 2.93E-8 YLL047W_d YLL047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
0.095 3.64E-8 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.095 4.12E-8 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.094 5.24E-8 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication