HIPHOP chemogenomics database
YOL066C / RIB2 
Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YOL066C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.116 | 1.72E-11 | YHR078W_p | YHR078W_p | hom | High osmolarity-regulated gene of unknown function |
| 0.103 | 1.88E-9 | YHR098C | SFB3 | hom | Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p |
| 0.095 | 3.75E-8 | YJR013W | GPI14 | het | Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M |
| 0.093 | 5.86E-8 | YGR193C | PDX1 | hom | Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core |
| 0.090 | 2.01E-7 | YJR062C | NTA1 | hom | Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation |
| 0.085 | 7.83E-7 | YBR160W | CDC28 | het | Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress |
| 0.085 | 8.45E-7 | YER052C | HOM3 | hom | Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis |
| 0.084 | 1.10E-6 | YLR182W | SWI6 | hom | Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators |
| 0.084 | 1.13E-6 | YMR007W_d | YMR007W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data |
| 0.084 | 1.25E-6 | YOR375C | GDH1 | hom | NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication |
| 0.083 | 1.71E-6 | YKL108W | SLD2 | het | Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress |
| 0.078 | 5.46E-6 | YGR224W | AZR1 | hom | Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole |
| 0.078 | 6.33E-6 | YOR029W_d | YOR029W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| 0.077 | 7.38E-6 | YIR008C | PRI1 | het | Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair |
| 0.075 | 1.57E-5 | YNL181W_p | YNL181W_p | het | Putative oxidoreductase; required for cell viability |