YOL084W / PHM7

Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL084W / PHM7

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:2.61|P-value:8.10E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.72|P-value:4.44E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:4.41|P-value:5.68E-8 ID:SGTC_933|Compound:2914-0560|FD-Score:5.64|P-value:5.81E-12 ID:SGTC_935|Compound:2996-0153|FD-Score:3.21|P-value:5.52E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.22|P-value:6.02E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.74|P-value:3.24E-6 ID:SGTC_1399|Compound:3562-5772|FD-Score:-2.88|P-value:2.96E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:-2.91|P-value:2.62E-4 ID:SGTC_1605|Compound:st000082|FD-Score:2.56|P-value:9.86E-4 ID:SGTC_1914|Compound:st062367|FD-Score:2.72|P-value:5.15E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.65|P-value:7.74E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-2.90|P-value:2.74E-4 ID:SGTC_2371|Compound:9071574|FD-Score:2.58|P-value:9.17E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.28|P-value:3.95E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.69|P-value:4.42E-6 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:2.72|P-value:5.05E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.01|P-value:1.44E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:2.83|P-value:3.23E-4 ID:SGTC_2809|Compound:7964133|FD-Score:-2.95|P-value:2.18E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.14|P-value:8.94E-5 ID:SGTC_2982|Compound:9019404|FD-Score:4.35|P-value:8.39E-8 ID:SGTC_3077|Compound:9117319|FD-Score:-2.67|P-value:7.16E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-2.96|P-value:2.03E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.42|P-value:1.92E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:2.61|P-value:8.10E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.72|P-value:4.44E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:4.41|P-value:5.68E-8 ID:SGTC_933|Compound:2914-0560|FD-Score:5.64|P-value:5.81E-12 ID:SGTC_935|Compound:2996-0153|FD-Score:3.21|P-value:5.52E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.22|P-value:6.02E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.74|P-value:3.24E-6 ID:SGTC_1399|Compound:3562-5772|FD-Score:-2.88|P-value:2.96E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:-2.91|P-value:2.62E-4 ID:SGTC_1605|Compound:st000082|FD-Score:2.56|P-value:9.86E-4 ID:SGTC_1914|Compound:st062367|FD-Score:2.72|P-value:5.15E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.65|P-value:7.74E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-2.90|P-value:2.74E-4 ID:SGTC_2371|Compound:9071574|FD-Score:2.58|P-value:9.17E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.28|P-value:3.95E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.69|P-value:4.42E-6 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:2.72|P-value:5.05E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.01|P-value:1.44E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:2.83|P-value:3.23E-4 ID:SGTC_2809|Compound:7964133|FD-Score:-2.95|P-value:2.18E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.14|P-value:8.94E-5 ID:SGTC_2982|Compound:9019404|FD-Score:4.35|P-value:8.39E-8 ID:SGTC_3077|Compound:9117319|FD-Score:-2.67|P-value:7.16E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-2.96|P-value:2.03E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.42|P-value:1.92E-5

Top fitness defect scores for YOL084W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_933 2914-0560 5.64 114.0 µM 5.81E-12 10 8.49E-9
2 SGTC_930 2810-3379 4.41 265.0 µM 5.68E-8 7 5.28E-6
3 SGTC_2982 9019404 4.35 26.0 µM 8.39E-8 16 6.94E-6
4 SGTC_1117 retinoic acid 3.74 1.1 µM 3.24E-6 20 9.06E-5
5 SGTC_2437 5741531 3.69 144.2 µM 4.42E-6 43 1.13E-4
6 SGTC_3197 9113331 3.42 49.5 µM 1.92E-5 51 3.17E-4
7 SGTC_2375 9072683 3.28 200.0 µM 3.95E-5 mitochondrial processes 34 5.28E-4
8 SGTC_935 2996-0153 3.21 430.0 µM 5.52E-5 PDR1 50 6.69E-4
9 SGTC_2620 irigenin 3.01 100.0 µM 1.44E-4 83 0.00132
10 SGTC_2621 lithochol-11-enic acid 2.83 100.0 µM 3.23E-4 95 0.00234
11 SGTC_2613 3',4'-dimethoxydalbergione 2.72 24.6 µM 5.05E-4 90 0.00322
12 SGTC_1914 st062367 2.72 72.6 µM 5.15E-4 86 0.00326
13 SGTC_39 0986-0283 2.61 58.4 µM 8.10E-4 calcium & mitochondrial duress 123 0.00450
14 SGTC_2371 9071574 2.58 200.0 µM 9.17E-4 74 0.00491
15 SGTC_1605 st000082 2.56 49.1 µM 9.86E-4 60S ribosome export 100 0.00518
16 SGTC_693 4161-2696 2.53 114.0 µM 0.00111 58 0.00562
17 SGTC_3207 9114163 2.53 49.5 µM 0.00114 142 0.00576
18 SGTC_548 1436-0017 2.51 110.0 µM 0.00122 TSC3-RPN4 147 0.00604
19 SGTC_2917 7979103 2.49 53.8 µM 0.00132 138 0.00637
20 SGTC_1434 4073-0095 2.49 163.0 µM 0.00133 85 0.00641

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 7.69E-26 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.174 3.25E-24 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.167 2.39E-22 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.135 4.05E-15 YKL141W SDH3 het Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
0.125 3.75E-13 YHR121W LSM12 hom Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
0.123 8.02E-13 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.121 1.81E-12 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.121 2.28E-12 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.117 1.31E-11 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.115 2.18E-11 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.112 7.04E-11 YDR510W SMT3 het Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
0.110 1.79E-10 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.108 3.16E-10 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.107 4.35E-10 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.106 7.71E-10 YNL266W_d YNL266W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C