YOL088C / MPD2

Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL088C / MPD2

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:7.15|P-value:3.92E-10 ID:SGTC_39|Compound:0986-0283|FD-Score:-4.20|P-value:7.47E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:4.11|P-value:2.45E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.10|P-value:2.54E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.18|P-value:1.98E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:-3.77|P-value:3.24E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.68|P-value:9.41E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.81|P-value:6.31E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:5.73|P-value:4.72E-7 ID:SGTC_389|Compound:tpck|FD-Score:4.54|P-value:5.60E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:3.69|P-value:9.25E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.71|P-value:8.71E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.73|P-value:8.04E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.73|P-value:8.21E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:5.94|P-value:1.79E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.84|P-value:2.55E-4 ID:SGTC_615|Compound:k087-0205|FD-Score:3.81|P-value:6.38E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:5.00|P-value:1.00E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:5.18|P-value:4.86E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:-7.90|P-value:1.09E-12 ID:SGTC_917|Compound:harmaline|FD-Score:3.76|P-value:7.35E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.74|P-value:7.90E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:5.77|P-value:4.04E-7 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.73|P-value:8.01E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.78|P-value:7.04E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.95|P-value:4.06E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.79|P-value:2.26E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:6.41|P-value:1.97E-8 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.72|P-value:8.30E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.95|P-value:4.16E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.84|P-value:1.87E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.93|P-value:4.34E-4 ID:SGTC_2049|Compound:5262831|FD-Score:5.68|P-value:5.96E-7 ID:SGTC_2152|Compound:5568786|FD-Score:5.78|P-value:3.74E-7 ID:SGTC_2181|Compound:6316240|FD-Score:4.87|P-value:1.65E-5 ID:SGTC_2207|Compound:7258803|FD-Score:5.22|P-value:4.24E-6 ID:SGTC_2243|Compound:6634756|FD-Score:3.70|P-value:8.84E-4 ID:SGTC_2296|Compound:7971656|FD-Score:6.47|P-value:1.43E-8 ID:SGTC_2308|Compound:7584454|FD-Score:-3.48|P-value:7.85E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.47|P-value:2.86E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.95|P-value:4.13E-4 ID:SGTC_2473|Compound:5854593|FD-Score:4.69|P-value:3.22E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:4.07|P-value:2.80E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:4.53|P-value:5.91E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.49|P-value:7.71E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-4.93|P-value:4.83E-6 ID:SGTC_2841|Compound:9004761|FD-Score:6.81|P-value:2.49E-9 ID:SGTC_2844|Compound:9010103|FD-Score:3.92|P-value:4.56E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-3.60|P-value:5.59E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.67|P-value:9.75E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.16|P-value:2.12E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.20|P-value:1.85E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.94|P-value:1.29E-5 ID:SGTC_3155|Compound:9099265|FD-Score:-3.92|P-value:2.01E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.86|P-value:5.47E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:7.15|P-value:3.92E-10 ID:SGTC_39|Compound:0986-0283|FD-Score:-4.20|P-value:7.47E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:4.11|P-value:2.45E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.10|P-value:2.54E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.18|P-value:1.98E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:-3.77|P-value:3.24E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.68|P-value:9.41E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.81|P-value:6.31E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:5.73|P-value:4.72E-7 ID:SGTC_389|Compound:tpck|FD-Score:4.54|P-value:5.60E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:3.69|P-value:9.25E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.71|P-value:8.71E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.73|P-value:8.04E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.73|P-value:8.21E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:5.94|P-value:1.79E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.84|P-value:2.55E-4 ID:SGTC_615|Compound:k087-0205|FD-Score:3.81|P-value:6.38E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:5.00|P-value:1.00E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:5.18|P-value:4.86E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:-7.90|P-value:1.09E-12 ID:SGTC_917|Compound:harmaline|FD-Score:3.76|P-value:7.35E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.74|P-value:7.90E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:5.77|P-value:4.04E-7 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.73|P-value:8.01E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.78|P-value:7.04E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.95|P-value:4.06E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.79|P-value:2.26E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:6.41|P-value:1.97E-8 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.72|P-value:8.30E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.95|P-value:4.16E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.84|P-value:1.87E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.93|P-value:4.34E-4 ID:SGTC_2049|Compound:5262831|FD-Score:5.68|P-value:5.96E-7 ID:SGTC_2152|Compound:5568786|FD-Score:5.78|P-value:3.74E-7 ID:SGTC_2181|Compound:6316240|FD-Score:4.87|P-value:1.65E-5 ID:SGTC_2207|Compound:7258803|FD-Score:5.22|P-value:4.24E-6 ID:SGTC_2243|Compound:6634756|FD-Score:3.70|P-value:8.84E-4 ID:SGTC_2296|Compound:7971656|FD-Score:6.47|P-value:1.43E-8 ID:SGTC_2308|Compound:7584454|FD-Score:-3.48|P-value:7.85E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.47|P-value:2.86E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.95|P-value:4.13E-4 ID:SGTC_2473|Compound:5854593|FD-Score:4.69|P-value:3.22E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:4.07|P-value:2.80E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:4.53|P-value:5.91E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.49|P-value:7.71E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-4.93|P-value:4.83E-6 ID:SGTC_2841|Compound:9004761|FD-Score:6.81|P-value:2.49E-9 ID:SGTC_2844|Compound:9010103|FD-Score:3.92|P-value:4.56E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-3.60|P-value:5.59E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.67|P-value:9.75E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.16|P-value:2.12E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.20|P-value:1.85E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.94|P-value:1.29E-5 ID:SGTC_3155|Compound:9099265|FD-Score:-3.92|P-value:2.01E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.86|P-value:5.47E-4

Top fitness defect scores for YOL088C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_16 erodoxin 7.15 22.8 µM 3.92E-10 unfolded protein response 16 4.21E-13
2 SGTC_2841 9004761 6.81 39.0 µM 2.49E-9 amide catabolism 5 4.86E-12
3 SGTC_2296 7971656 6.47 126.5 µM 1.43E-8 10 4.88E-11
4 SGTC_1587 estradiol valerate 6.41 56.1 µM 1.97E-8 2 7.47E-11
5 SGTC_533 1222-0222 5.94 6.7 µM 1.79E-7 PDR1 6 1.38E-9
6 SGTC_2152 5568786 5.78 200.0 µM 3.74E-7 2 3.66E-9
7 SGTC_1131 3454-3128 5.77 32.3 µM 4.04E-7 7 4.06E-9
8 SGTC_287 k060-0039 5.73 29.6 µM 4.72E-7 2 4.99E-9
9 SGTC_2049 5262831 5.68 67.2 µM 5.96E-7 6 6.79E-9
10 SGTC_2207 7258803 5.22 200.0 µM 4.24E-6 1 9.12E-8
11 SGTC_816 0325-0417 5.18 81.3 µM 4.86E-6 2 1.09E-7
12 SGTC_799 1310-0557 5.00 151.0 µM 1.00E-5 superoxide 10 2.84E-7
13 SGTC_3068 9116768 4.94 49.5 µM 1.29E-5 8 3.96E-7
14 SGTC_2181 6316240 4.87 54.3 µM 1.65E-5 10 5.50E-7
15 SGTC_1622 st003707 4.84 19.4 µM 1.87E-5 calcium & mitochondrial duress 16 6.49E-7
16 SGTC_1577 3(2'-chlorophenyl)-7-ethoxycoumarin 4.79 66.5 µM 2.26E-5 9 8.36E-7
17 SGTC_2473 5854593 4.69 8.9 µM 3.22E-5 3 1.34E-6
18 SGTC_389 tpck 4.54 16.7 µM 5.60E-5 21 2.78E-6
19 SGTC_2687 chenodiol 4.53 100.0 µM 5.91E-5 12 2.98E-6
20 SGTC_3009 9080819 4.20 71.4 µM 1.85E-4 12 1.35E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.74E-13 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.122 1.21E-12 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.108 3.30E-10 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.102 2.72E-9 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.099 8.27E-9 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.097 1.57E-8 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.083 1.31E-6 YBR007C DSF2 hom Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.083 1.37E-6 YBL100W-C_p YBL100W-C_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.083 1.35E-6 YOR316C COT1 hom Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
0.083 1.47E-6 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.081 2.66E-6 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.081 2.88E-6 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.081 2.93E-6 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.079 4.79E-6 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.076 9.31E-6 YLR421C RPN13 hom Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress