YOL135C / MED7

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL135C / MED7

Click on Significant Values for Screen Details ID:SGTC_124|Compound:0136-0217|FD-Score:3.08|P-value:9.53E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.48|P-value:2.29E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.37|P-value:7.43E-11 ID:SGTC_227|Compound:fluconazole|FD-Score:4.53|P-value:2.54E-6 ID:SGTC_263|Compound:alizarin|FD-Score:3.84|P-value:5.53E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:9.03|P-value:5.37E-20 ID:SGTC_302|Compound:1094-0063|FD-Score:3.63|P-value:1.29E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.02|P-value:2.79E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.22|P-value:6.28E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:7.04|P-value:7.19E-13 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.41|P-value:5.70E-11 ID:SGTC_505|Compound:gw-5074|FD-Score:5.35|P-value:3.69E-8 ID:SGTC_568|Compound:1193-0097|FD-Score:3.10|P-value:9.04E-4 ID:SGTC_643|Compound:k048-0106|FD-Score:4.03|P-value:2.53E-5 ID:SGTC_652|Compound:0437-0092|FD-Score:-3.38|P-value:3.61E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.43|P-value:2.74E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.09|P-value:9.38E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.57|P-value:1.61E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.15|P-value:7.43E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.52|P-value:2.13E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:-3.13|P-value:8.53E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.10|P-value:9.06E-4 ID:SGTC_1603|Compound:st002045|FD-Score:6.61|P-value:1.45E-11 ID:SGTC_1637|Compound:st007924|FD-Score:-4.65|P-value:1.60E-6 ID:SGTC_1687|Compound:st020778|FD-Score:3.36|P-value:3.63E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.15|P-value:1.48E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.20|P-value:6.32E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:6.11|P-value:4.09E-10 ID:SGTC_1810|Compound:st050138|FD-Score:3.33|P-value:4.04E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.10|P-value:9.58E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.18|P-value:7.14E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.92|P-value:4.20E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.49|P-value:2.32E-4 ID:SGTC_2107|Compound:5363541|FD-Score:4.00|P-value:2.81E-5 ID:SGTC_2109|Compound:5483026|FD-Score:4.05|P-value:2.32E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.14|P-value:7.81E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.60|P-value:1.46E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.22|P-value:5.82E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-4.85|P-value:5.72E-7 ID:SGTC_2876|Compound:9059903|FD-Score:3.44|P-value:2.64E-4 ID:SGTC_3011|Compound:9081274|FD-Score:-3.10|P-value:9.52E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.10|P-value:8.93E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.14|P-value:7.77E-4 ID:SGTC_3338|Compound:9144300|FD-Score:-3.49|P-value:2.32E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.08|P-value:9.53E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.48|P-value:2.29E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.37|P-value:7.43E-11 ID:SGTC_227|Compound:fluconazole|FD-Score:4.53|P-value:2.54E-6 ID:SGTC_263|Compound:alizarin|FD-Score:3.84|P-value:5.53E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:9.03|P-value:5.37E-20 ID:SGTC_302|Compound:1094-0063|FD-Score:3.63|P-value:1.29E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.02|P-value:2.79E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.22|P-value:6.28E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:7.04|P-value:7.19E-13 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.41|P-value:5.70E-11 ID:SGTC_505|Compound:gw-5074|FD-Score:5.35|P-value:3.69E-8 ID:SGTC_568|Compound:1193-0097|FD-Score:3.10|P-value:9.04E-4 ID:SGTC_643|Compound:k048-0106|FD-Score:4.03|P-value:2.53E-5 ID:SGTC_652|Compound:0437-0092|FD-Score:-3.38|P-value:3.61E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.43|P-value:2.74E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.09|P-value:9.38E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.57|P-value:1.61E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.15|P-value:7.43E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.52|P-value:2.13E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:-3.13|P-value:8.53E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.10|P-value:9.06E-4 ID:SGTC_1603|Compound:st002045|FD-Score:6.61|P-value:1.45E-11 ID:SGTC_1637|Compound:st007924|FD-Score:-4.65|P-value:1.60E-6 ID:SGTC_1687|Compound:st020778|FD-Score:3.36|P-value:3.63E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.15|P-value:1.48E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.20|P-value:6.32E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:6.11|P-value:4.09E-10 ID:SGTC_1810|Compound:st050138|FD-Score:3.33|P-value:4.04E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.10|P-value:9.58E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.18|P-value:7.14E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.92|P-value:4.20E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.49|P-value:2.32E-4 ID:SGTC_2107|Compound:5363541|FD-Score:4.00|P-value:2.81E-5 ID:SGTC_2109|Compound:5483026|FD-Score:4.05|P-value:2.32E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.14|P-value:7.81E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.60|P-value:1.46E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.22|P-value:5.82E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-4.85|P-value:5.72E-7 ID:SGTC_2876|Compound:9059903|FD-Score:3.44|P-value:2.64E-4 ID:SGTC_3011|Compound:9081274|FD-Score:-3.10|P-value:9.52E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.10|P-value:8.93E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.14|P-value:7.77E-4 ID:SGTC_3338|Compound:9144300|FD-Score:-3.49|P-value:2.32E-4

Top fitness defect scores for YOL135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_268 camptothecin 9.03 39.6 µM 5.37E-20 DNA damage response 8 8.37E-20 0.13
2 SGTC_431 k089-0097 7.04 107.0 µM 7.19E-13 iron homeostasis 2 9.57E-13 0.21
3 SGTC_1603 st002045 6.61 67.8 µM 1.45E-11 TSC3-RPN4 2 1.87E-11 3.20
4 SGTC_453 enantio-paf c16 6.41 10.0 µM 5.70E-11 3 7.26E-11 0.33
5 SGTC_173 1273-0061 6.37 2.7 µM 7.43E-11 mitochondrial stress 7 9.46E-11 0.78
6 SGTC_1787 fluconazole 6.11 80.0 µM 4.09E-10 iron homeostasis 1 5.12E-10 0.55
7 SGTC_505 gw-5074 5.35 63.9 µM 3.69E-8 3 4.41E-8 0.23
8 SGTC_227 fluconazole 4.53 33.5 µM 2.54E-6 azole & statin 41 2.92E-6 0.02
9 SGTC_1707 st032323 4.15 6.3 µM 1.48E-5 DNA damage response 28 1.67E-5 0.21
10 SGTC_2109 5483026 4.05 200.0 µM 2.32E-5 RSC complex & mRNA processing 33 2.60E-5 0.04
11 SGTC_643 k048-0106 4.03 28.4 µM 2.53E-5 RSC & ERG11 15 2.83E-5 0.29
12 SGTC_2107 5363541 4.00 170.2 µM 2.81E-5 7 3.14E-5 0.21
13 SGTC_263 alizarin 3.84 107.9 µM 5.53E-5 iron homeostasis 12 6.15E-5 0.02
14 SGTC_302 1094-0063 3.63 18.9 µM 1.29E-4 21 1.42E-4 0.06
15 SGTC_2396 5342950 3.60 42.8 µM 1.46E-4 RSC complex & mRNA processing 42 1.60E-4 0.03
16 SGTC_783 0848-0141 3.57 92.7 µM 1.61E-4 RSC & ERG11 20 1.77E-4 0.06
17 SGTC_145 3737-0113 3.48 38.4 µM 2.29E-4 DNA intercalators 62 2.50E-4 0.04
18 SGTC_2876 9059903 3.44 26.0 µM 2.64E-4 3 2.89E-4 0.24
19 SGTC_709 3807-1509 3.43 243.0 µM 2.74E-4 RSC complex & mRNA processing 20 2.99E-4 0.02
20 SGTC_682 0kpi-0099 3.36 17.0 µM 3.58E-4 46 3.90E-4 6.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.201 6.03E-32 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.159 2.39E-20 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.156 8.62E-20 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.155 1.64E-19 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.155 1.67E-19 YDR436W PPZ2 hom Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.152 6.68E-19 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.150 2.26E-18 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.149 5.08E-18 YOL134C_d YOL134C_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
0.148 5.16E-18 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.145 3.25E-17 YLR321C SFH1 het Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
0.145 4.00E-17 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.144 6.07E-17 YPL003W ULA1 hom Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
0.143 7.28E-17 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.138 1.10E-15 YHR058C MED6 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
0.136 2.34E-15 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway