YOL141W / PPM2

AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL141W / PPM2

Click on Significant Values for Screen Details ID:SGTC_333|Compound:3486-0131|FD-Score:2.73|P-value:8.04E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-5.06|P-value:5.19E-9 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.46|P-value:4.84E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-5.00|P-value:8.08E-9 ID:SGTC_632|Compound:0781-2353|FD-Score:2.89|P-value:4.22E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.73|P-value:4.68E-8 ID:SGTC_634|Compound:1611-4317|FD-Score:3.70|P-value:1.02E-5 ID:SGTC_636|Compound:0kpi-0074|FD-Score:-4.44|P-value:2.71E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.01|P-value:5.14E-12 ID:SGTC_808|Compound:0711-0032|FD-Score:3.41|P-value:4.26E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:-3.06|P-value:2.85E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-2.82|P-value:7.67E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.10|P-value:2.42E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-2.88|P-value:6.00E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.15|P-value:1.38E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.80|P-value:8.25E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:2.79|P-value:6.39E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.70|P-value:9.09E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.49|P-value:4.27E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.33|P-value:9.10E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.94|P-value:3.45E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.96|P-value:3.18E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.82|P-value:7.84E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-3.26|P-value:1.20E-4 ID:SGTC_2280|Compound:7785178|FD-Score:-3.72|P-value:1.38E-5 ID:SGTC_2293|Compound:7624732|FD-Score:-3.22|P-value:1.43E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.07|P-value:2.00E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.06|P-value:2.07E-4 ID:SGTC_2804|Compound:7954240|FD-Score:5.45|P-value:1.97E-10 ID:SGTC_3206|Compound:9114350|FD-Score:-4.46|P-value:2.36E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-3.69|P-value:1.60E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:2.73|P-value:8.04E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-5.06|P-value:5.19E-9 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.46|P-value:4.84E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-5.00|P-value:8.08E-9 ID:SGTC_632|Compound:0781-2353|FD-Score:2.89|P-value:4.22E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.73|P-value:4.68E-8 ID:SGTC_634|Compound:1611-4317|FD-Score:3.70|P-value:1.02E-5 ID:SGTC_636|Compound:0kpi-0074|FD-Score:-4.44|P-value:2.71E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.01|P-value:5.14E-12 ID:SGTC_808|Compound:0711-0032|FD-Score:3.41|P-value:4.26E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:-3.06|P-value:2.85E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-2.82|P-value:7.67E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.10|P-value:2.42E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-2.88|P-value:6.00E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.15|P-value:1.38E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.80|P-value:8.25E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:2.79|P-value:6.39E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.70|P-value:9.09E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.49|P-value:4.27E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.33|P-value:9.10E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.94|P-value:3.45E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.96|P-value:3.18E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.82|P-value:7.84E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-3.26|P-value:1.20E-4 ID:SGTC_2280|Compound:7785178|FD-Score:-3.72|P-value:1.38E-5 ID:SGTC_2293|Compound:7624732|FD-Score:-3.22|P-value:1.43E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.07|P-value:2.00E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.06|P-value:2.07E-4 ID:SGTC_2804|Compound:7954240|FD-Score:5.45|P-value:1.97E-10 ID:SGTC_3206|Compound:9114350|FD-Score:-4.46|P-value:2.36E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-3.69|P-value:1.60E-5

Top fitness defect scores for YOL141W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2804 7954240 5.45 19.5 µM 1.97E-10 8 2.49E-8
2 SGTC_634 1611-4317 3.70 11.2 µM 1.02E-5 ERG2 20 1.08E-4
3 SGTC_808 0711-0032 3.41 135.0 µM 4.26E-5 31 3.26E-4
4 SGTC_933 2914-0560 3.15 114.0 µM 1.38E-4 59 8.09E-4
5 SGTC_2435 5706476 3.07 10.6 µM 2.00E-4 62 0.00108
6 SGTC_2754 granisetron 3.06 64.0 µM 2.07E-4 85 0.00111
7 SGTC_1270 0831-1170 2.96 17.3 µM 3.18E-4 51 0.00155
8 SGTC_1263 0774-1208 2.94 64.8 µM 3.45E-4 121 0.00165
9 SGTC_632 0781-2353 2.89 4.7 µM 4.22E-4 Golgi 92 0.00193
10 SGTC_968 1309-0633 2.79 340.0 µM 6.39E-4 63 0.00266
11 SGTC_333 3486-0131 2.73 40.1 µM 8.04E-4 amide catabolism 57 0.00318
12 SGTC_988 1497-1057 2.70 103.0 µM 9.09E-4 66 0.00350
13 SGTC_39 0986-0283 2.60 58.4 µM 0.00133 calcium & mitochondrial duress 129 0.00470
14 SGTC_3209 9113616 2.54 49.5 µM 0.00163 128 0.00551
15 SGTC_784 4579-0127 2.54 173.0 µM 0.00167 106 0.00562
16 SGTC_2103 5378138 2.52 10.0 µM 0.00175 113 0.00581
17 SGTC_1322 1348-1070 2.52 12.0 µM 0.00178 63 0.00589
18 SGTC_1183 1254-0324 2.52 198.0 µM 0.00179 96 0.00591
19 SGTC_621 3584-0052 2.50 32.1 µM 0.00189 120 0.00617
20 SGTC_1679 4130-1276 2.49 6.7 µM 0.00200 Golgi 183 0.00646

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.423 1.66E-145 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.317 6.77E-79 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.296 8.22E-69 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.286 2.29E-64 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.267 1.05E-55 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.249 1.30E-48 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.239 6.28E-45 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.234 7.79E-43 YLL046C RNP1 hom Ribonucleoprotein that contains two RNA recognition motifs (RRM)
0.232 2.49E-42 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.231 5.70E-42 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.229 4.61E-41 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.227 2.70E-40 YOR345C_d YOR345C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.220 3.92E-38 YGL134W PCL10 hom Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate
0.219 1.26E-37 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.218 2.13E-37 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth