HIPHOP chemogenomics database

YOL146W / PSF3

Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOL146W / PSF3

Click on Significant Values for Screen Details

Top fitness defect scores for YOL146W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2413	inermin	16.20	26.1 µM	1.86E-62		5	1.96E-59	6.57
2	SGTC_91	0417-1751	12.00	58.4 µM	2.26E-35	Golgi	1	1.05E-33	3.89
3	SGTC_81	0411-0046	10.80	10.0 µM	7.95E-29		6	1.75E-27	0.54
4	SGTC_878	0828-0289	10.80	13.7 µM	1.26E-28		4	2.71E-27	0.44
5	SGTC_964	1287-0107	9.25	19.4 µM	1.18E-21	cell wall	2	1.14E-20	1.69
6	SGTC_389	tpck	8.65	16.7 µM	3.55E-19		3	2.59E-18	3.73
7	SGTC_834	0312-0008	5.40	75.7 µM	1.50E-8	NEO1	6	3.29E-8	0.21
8	SGTC_3219	9131112	4.88	49.5 µM	2.72E-7	Golgi	11	5.17E-7	0.01
9	SGTC_1679	4130-1276	4.75	6.7 µM	5.63E-7	Golgi	18	1.03E-6	0.10
10	SGTC_1638	st008370	4.49	60.6 µM	2.08E-6	ergosterol biosynthesis	14	3.58E-6	0.18
11	SGTC_632	0781-2353	4.29	4.7 µM	5.36E-6	Golgi	15	8.83E-6	0.34
12	SGTC_3072	9116419	4.14	49.5 µM	1.09E-5	ERG2	4	1.73E-5	0.41
13	SGTC_2363	9053361	4.05	176.0 µM	1.60E-5	Golgi	11	2.51E-5	0.44
14	SGTC_2982	9019404	3.91	26.0 µM	2.99E-5		5	4.54E-5	0.59
15	SGTC_1081	sertraline	3.89	6.0 µM	3.32E-5	60S ribosome export	7	5.02E-5	0.08
16	SGTC_2090	5301565	3.88	104.5 µM	3.50E-5	TSC3-RPN4	6	5.28E-5	0.28
17	SGTC_1306	1185-0270	3.81	9.0 µM	4.65E-5	Golgi	11	6.92E-5	0.02
18	SGTC_1783	st039459	3.76	87.5 µM	5.76E-5	Golgi	10	8.49E-5	0.31
19	SGTC_3103	9120822	3.75	49.5 µM	5.96E-5		10	8.77E-5	0.30
20	SGTC_2846	9008642	3.72	19.5 µM	6.88E-5		9	1.01E-4	0.00

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.553	8.42E-269	YFL038C	YPT1	het	Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.292	3.57E-67	YDR108W	TRS85	hom	Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
0.218	1.77E-37	YBR164C	ARL1	hom	Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.203	1.05E-32	YGL113W	SLD3	het	Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.194	1.07E-29	YBR243C	ALG7	het	UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.193	1.36E-29	YPL051W	ARL3	hom	GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.191	7.10E-29	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.187	8.86E-28	YOR246C	ENV9	hom	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.179	1.92E-25	YDR162C	NBP2	hom	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.170	4.18E-23	YGL005C	COG7	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.164	1.05E-21	YMR079W	SEC14	het	Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.157	7.20E-20	YML071C	COG8	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.147	8.79E-18	YOL149W	DCP1	het	Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
0.142	1.34E-16	YML004C	GLO1	hom	Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
0.141	2.25E-16	YPL082C	MOT1	het	Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA