YOL158C / ENB1

Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL158C / ENB1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.32|P-value:1.27E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:3.39|P-value:3.28E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.37|P-value:1.06E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:4.44|P-value:4.45E-6 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.79|P-value:7.13E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.33|P-value:4.12E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.79|P-value:1.12E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.14|P-value:7.80E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.32|P-value:4.26E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.21|P-value:6.12E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.90|P-value:4.61E-5 ID:SGTC_1737|Compound:st035557|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_1852|Compound:st056254|FD-Score:-4.93|P-value:7.65E-7 ID:SGTC_1857|Compound:st056007|FD-Score:-4.16|P-value:2.58E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-4.05|P-value:4.10E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.44|P-value:4.15E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.32|P-value:4.28E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.24|P-value:5.48E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.81|P-value:6.46E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.51|P-value:2.07E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.87|P-value:5.24E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.85|P-value:5.60E-5 ID:SGTC_3192|Compound:9110362|FD-Score:-3.47|P-value:3.79E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.68|P-value:1.73E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.32|P-value:1.27E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:3.39|P-value:3.28E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.37|P-value:1.06E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:4.44|P-value:4.45E-6 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.79|P-value:7.13E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.33|P-value:4.12E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.79|P-value:1.12E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.14|P-value:7.80E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.32|P-value:4.26E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.21|P-value:6.12E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.90|P-value:4.61E-5 ID:SGTC_1737|Compound:st035557|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_1852|Compound:st056254|FD-Score:-4.93|P-value:7.65E-7 ID:SGTC_1857|Compound:st056007|FD-Score:-4.16|P-value:2.58E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-4.05|P-value:4.10E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.44|P-value:4.15E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.32|P-value:4.28E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.24|P-value:5.48E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.81|P-value:6.46E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.51|P-value:2.07E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.87|P-value:5.24E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.85|P-value:5.60E-5 ID:SGTC_3192|Compound:9110362|FD-Score:-3.47|P-value:3.79E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.68|P-value:1.73E-4

Top fitness defect scores for YOL158C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_114 0364-0001 4.44 490.3 µM 4.45E-6 33 4.57E-6
2 SGTC_1727 st034309 3.90 9.9 µM 4.61E-5 43 4.84E-5
3 SGTC_2576 guaiol 3.87 23.1 µM 5.24E-5 22 5.51E-5
4 SGTC_2693 st077581 3.85 40.7 µM 5.60E-5 38 5.90E-5
5 SGTC_2504 purpurogallin 3.81 52.3 µM 6.46E-5 RPP1 & pyrimidine depletion 24 6.82E-5
6 SGTC_353 1988-1175 3.79 58.3 µM 7.13E-5 unfolded protein response 29 7.53E-5
7 SGTC_2567 aureobasidin a 3.51 60.0 nM 2.07E-4 114 2.20E-4
8 SGTC_1737 st035557 3.39 69.7 µM 3.21E-4 27 3.43E-4
9 SGTC_74 000s-0487 3.39 160.3 µM 3.28E-4 38 3.50E-4
10 SGTC_539 4859-0462 3.33 2.5 µM 4.12E-4 RNA pol III & RNase P/MRP 49 4.42E-4
11 SGTC_219 mitoxanthrone 3.32 164.4 µM 4.16E-4 DNA intercalators 69 4.45E-4
12 SGTC_817 1391-0715 3.32 46.0 µM 4.26E-4 35 4.56E-4
13 SGTC_2341 7987486 3.32 176.7 µM 4.28E-4 35 4.58E-4
14 SGTC_2346 9031566 3.24 143.8 µM 5.48E-4 Golgi 50 5.88E-4
15 SGTC_1125 tnp00248 3.21 1.3 µM 6.12E-4 77 6.58E-4
16 SGTC_649 1123-0311 3.14 367.0 µM 7.80E-4 endomembrane recycling 118 8.39E-4
17 SGTC_756 0508-1999 3.06 28.3 µM 0.00103 RPP1 & pyrimidine depletion 65 0.00111
18 SGTC_716 1683-5115 3.02 658.0 µM 0.00117 62 0.00126
19 SGTC_2764 methyldopa 3.00 94.7 µM 0.00124 70 0.00134
20 SGTC_2506 ethyl hematommate 2.93 54.2 µM 0.00155 RPP1 & pyrimidine depletion 64 0.00168

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 4.43E-23 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.138 1.24E-15 YJL118W YJL118W hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
0.124 5.10E-13 YDL180W_p YDL180W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.118 5.98E-12 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.117 1.17E-11 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.109 2.52E-10 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.109 2.75E-10 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.107 4.21E-10 YDR156W RPA14 hom RNA polymerase I subunit A14
0.106 7.47E-10 YGR124W ASN2 hom Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
0.098 1.57E-8 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.096 2.19E-8 YDL053C PBP4 hom Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.096 2.85E-8 YOR044W IRC23 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication
0.095 3.27E-8 YGR154C GTO1 hom Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
0.095 3.84E-8 YAL028W FRT2 hom Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
0.093 5.87E-8 YJR154W_p YJR154W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm