YOL159C-A

Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL159C-A / YOL159C-A

Click on Significant Values for Screen Details ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.67|P-value:7.61E-27 ID:SGTC_236|Compound:1509-0011|FD-Score:3.64|P-value:2.92E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:2.92|P-value:6.27E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:2.82|P-value:8.98E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:5.14|P-value:7.21E-9 ID:SGTC_825|Compound:1988-2224|FD-Score:3.92|P-value:7.35E-6 ID:SGTC_940|Compound:3232-1287|FD-Score:3.65|P-value:2.80E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:5.23|P-value:3.79E-9 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.37|P-value:1.12E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.31|P-value:1.46E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.15|P-value:2.31E-6 ID:SGTC_1182|Compound:1222-0449|FD-Score:-3.09|P-value:3.58E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.42|P-value:7.90E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:2.95|P-value:5.45E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.45|P-value:6.95E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.38|P-value:9.20E-5 ID:SGTC_1362|Compound:1611-0397|FD-Score:2.80|P-value:9.61E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:2.81|P-value:9.46E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.14|P-value:2.60E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.58|P-value:3.82E-5 ID:SGTC_1621|Compound:st003705|FD-Score:2.95|P-value:5.41E-4 ID:SGTC_1635|Compound:st007035|FD-Score:2.82|P-value:9.18E-4 ID:SGTC_1636|Compound:st007707|FD-Score:2.98|P-value:4.84E-4 ID:SGTC_1685|Compound:st024049|FD-Score:2.93|P-value:5.83E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.49|P-value:5.75E-5 ID:SGTC_1712|Compound:st032288|FD-Score:2.99|P-value:4.69E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.39|P-value:9.00E-5 ID:SGTC_1760|Compound:st045453|FD-Score:4.09|P-value:3.17E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.23|P-value:2.04E-4 ID:SGTC_1781|Compound:st038725|FD-Score:6.67|P-value:9.63E-14 ID:SGTC_1800|Compound:st050079|FD-Score:5.44|P-value:9.84E-10 ID:SGTC_1803|Compound:st050068|FD-Score:4.76|P-value:7.19E-8 ID:SGTC_1836|Compound:st055642|FD-Score:3.34|P-value:1.09E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.13|P-value:2.64E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.90|P-value:8.34E-6 ID:SGTC_1910|Compound:st062352|FD-Score:4.21|P-value:1.69E-6 ID:SGTC_1991|Compound:st073000|FD-Score:5.63|P-value:2.52E-10 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.26|P-value:1.56E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.20|P-value:1.78E-6 ID:SGTC_2335|Compound:9018011|FD-Score:2.79|P-value:9.93E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.50|P-value:5.56E-5 ID:SGTC_2345|Compound:9052315|FD-Score:4.34|P-value:8.00E-7 ID:SGTC_2469|Compound:5655019|FD-Score:3.14|P-value:2.56E-4 ID:SGTC_2474|Compound:5321570|FD-Score:2.85|P-value:8.01E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.26|P-value:3.13E-9 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.01|P-value:4.77E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:3.85|P-value:1.03E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.42|P-value:7.94E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.26|P-value:1.26E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:2.96|P-value:5.36E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.19|P-value:1.87E-6 ID:SGTC_2789|Compound:5281112|FD-Score:3.55|P-value:4.41E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.22|P-value:1.81E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.94|P-value:2.51E-8 ID:SGTC_2900|Compound:5328528|FD-Score:-2.89|P-value:7.94E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-3.53|P-value:5.63E-5 ID:SGTC_2957|Compound:9076800|FD-Score:-5.37|P-value:1.87E-9 ID:SGTC_2962|Compound:9082311|FD-Score:-3.38|P-value:1.09E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.88|P-value:3.67E-8 ID:SGTC_3281|Compound:9140613|FD-Score:5.11|P-value:8.67E-9 ID:SGTC_3299|Compound:9121982|FD-Score:-2.91|P-value:7.21E-4 ID:SGTC_3306|Compound:9127676|FD-Score:-3.46|P-value:7.52E-5 ID:SGTC_3333|Compound:9141820|FD-Score:-3.31|P-value:1.44E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.67|P-value:7.61E-27 ID:SGTC_236|Compound:1509-0011|FD-Score:3.64|P-value:2.92E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:2.92|P-value:6.27E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:2.82|P-value:8.98E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:5.14|P-value:7.21E-9 ID:SGTC_825|Compound:1988-2224|FD-Score:3.92|P-value:7.35E-6 ID:SGTC_940|Compound:3232-1287|FD-Score:3.65|P-value:2.80E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:5.23|P-value:3.79E-9 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.37|P-value:1.12E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.31|P-value:1.46E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.15|P-value:2.31E-6 ID:SGTC_1182|Compound:1222-0449|FD-Score:-3.09|P-value:3.58E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.42|P-value:7.90E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:2.95|P-value:5.45E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.45|P-value:6.95E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.38|P-value:9.20E-5 ID:SGTC_1362|Compound:1611-0397|FD-Score:2.80|P-value:9.61E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:2.81|P-value:9.46E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.14|P-value:2.60E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.58|P-value:3.82E-5 ID:SGTC_1621|Compound:st003705|FD-Score:2.95|P-value:5.41E-4 ID:SGTC_1635|Compound:st007035|FD-Score:2.82|P-value:9.18E-4 ID:SGTC_1636|Compound:st007707|FD-Score:2.98|P-value:4.84E-4 ID:SGTC_1685|Compound:st024049|FD-Score:2.93|P-value:5.83E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.49|P-value:5.75E-5 ID:SGTC_1712|Compound:st032288|FD-Score:2.99|P-value:4.69E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.39|P-value:9.00E-5 ID:SGTC_1760|Compound:st045453|FD-Score:4.09|P-value:3.17E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.23|P-value:2.04E-4 ID:SGTC_1781|Compound:st038725|FD-Score:6.67|P-value:9.63E-14 ID:SGTC_1800|Compound:st050079|FD-Score:5.44|P-value:9.84E-10 ID:SGTC_1803|Compound:st050068|FD-Score:4.76|P-value:7.19E-8 ID:SGTC_1836|Compound:st055642|FD-Score:3.34|P-value:1.09E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.13|P-value:2.64E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.90|P-value:8.34E-6 ID:SGTC_1910|Compound:st062352|FD-Score:4.21|P-value:1.69E-6 ID:SGTC_1991|Compound:st073000|FD-Score:5.63|P-value:2.52E-10 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.26|P-value:1.56E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.20|P-value:1.78E-6 ID:SGTC_2335|Compound:9018011|FD-Score:2.79|P-value:9.93E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.50|P-value:5.56E-5 ID:SGTC_2345|Compound:9052315|FD-Score:4.34|P-value:8.00E-7 ID:SGTC_2469|Compound:5655019|FD-Score:3.14|P-value:2.56E-4 ID:SGTC_2474|Compound:5321570|FD-Score:2.85|P-value:8.01E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.26|P-value:3.13E-9 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.01|P-value:4.77E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:3.85|P-value:1.03E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.42|P-value:7.94E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.26|P-value:1.26E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:2.96|P-value:5.36E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.19|P-value:1.87E-6 ID:SGTC_2789|Compound:5281112|FD-Score:3.55|P-value:4.41E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.22|P-value:1.81E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.94|P-value:2.51E-8 ID:SGTC_2900|Compound:5328528|FD-Score:-2.89|P-value:7.94E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-3.53|P-value:5.63E-5 ID:SGTC_2957|Compound:9076800|FD-Score:-5.37|P-value:1.87E-9 ID:SGTC_2962|Compound:9082311|FD-Score:-3.38|P-value:1.09E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.88|P-value:3.67E-8 ID:SGTC_3281|Compound:9140613|FD-Score:5.11|P-value:8.67E-9 ID:SGTC_3299|Compound:9121982|FD-Score:-2.91|P-value:7.21E-4 ID:SGTC_3306|Compound:9127676|FD-Score:-3.46|P-value:7.52E-5 ID:SGTC_3333|Compound:9141820|FD-Score:-3.31|P-value:1.44E-4

Top fitness defect scores for YOL159C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 9.67 10.3 µM 7.61E-27 NEO1-PIK1 63 1.93E-22
2 SGTC_1781 st038725 6.67 66.3 µM 9.63E-14 NEO1 47 1.30E-11
3 SGTC_1991 st073000 5.63 8.9 µM 2.52E-10 NEO1 41 8.77E-9
4 SGTC_1800 st050079 5.44 29.1 µM 9.84E-10 NEO1-PIK1 59 2.71E-8
5 SGTC_2563 tomatine 5.26 8.5 µM 3.13E-9 19 7.06E-8
6 SGTC_942 3253-1115 5.23 227.0 µM 3.79E-9 8 8.27E-8
7 SGTC_814 3342-0569 5.14 96.0 µM 7.21E-9 7 1.41E-7
8 SGTC_3281 9140613 5.11 49.5 µM 8.67E-9 RPP1 & pyrimidine depletion 6 1.64E-7
9 SGTC_2835 9006588 4.94 71.4 µM 2.51E-8 20 3.96E-7
10 SGTC_3037 9091026 4.88 49.5 µM 3.67E-8 NEO1-PIK1 79 5.42E-7
11 SGTC_1803 st050068 4.76 52.4 µM 7.19E-8 NEO1-PIK1 127 9.47E-7
12 SGTC_2345 9052315 4.34 157.4 µM 8.00E-7 16 6.97E-6
13 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.26 100.0 µM 1.26E-6 30 1.02E-5
14 SGTC_1910 st062352 4.21 60.4 µM 1.69E-6 25 1.30E-5
15 SGTC_2257 7960413 4.20 200.0 µM 1.78E-6 NEO1-PIK1 49 1.36E-5
16 SGTC_2688 kanamycin b 4.19 96.7 µM 1.87E-6 calcium & mitochondrial duress 336 1.41E-5
17 SGTC_1113 artemisinin 4.15 1.2 µM 2.31E-6 heme biosynthesis & mitochondrial translocase 12 1.68E-5
18 SGTC_1760 st045453 4.09 10.4 µM 3.17E-6 NEO1-PIK1 141 2.19E-5
19 SGTC_2564 betulinic acid 4.01 100.0 µM 4.77E-6 29 3.07E-5
20 SGTC_825 1988-2224 3.92 89.6 µM 7.35E-6 cell wall signaling 35 4.40E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.308 1.17E-74 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.302 2.19E-71 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.285 1.37E-63 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.277 5.16E-60 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.276 8.78E-60 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.274 9.08E-59 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.272 1.02E-57 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.268 2.87E-56 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.267 7.66E-56 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.260 1.11E-52 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.255 6.83E-51 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.253 6.54E-50 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.253 7.08E-50 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.252 1.18E-49 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.246 2.22E-47 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions