HIPHOP chemogenomics database

YOR002W / ALG6

Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR002W / ALG6

Click on Significant Values for Screen Details

Top fitness defect scores for YOR002W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2289	7946404	10.80	165.1 µM	1.68E-20		7	2.91E-27
2	SGTC_143	4032-1194	8.02	15.7 µM	3.57E-12		9	5.23E-16
3	SGTC_2009	4012297	7.43	12.1 µM	1.10E-10	cell wall signaling	16	5.40E-14
4	SGTC_3076	9116187	7.22	49.5 µM	3.56E-10		2	2.63E-13
5	SGTC_3208	9114115	6.47	49.5 µM	1.68E-8		8	4.80E-11
6	SGTC_72	0744-0168	6.33	198.4 µM	3.35E-8	RPP1 & pyrimidine depletion	6	1.21E-10
7	SGTC_1597	7-benzyloxygramine	5.89	47.5 µM	2.63E-7	calcium & mitochondrial duress	53	1.96E-9
8	SGTC_830	4076-0286	5.88	57.8 µM	2.71E-7	translation	18	2.04E-9
9	SGTC_2896	9052656	5.86	71.4 µM	3.01E-7	endomembrane recycling	41	2.36E-9
10	SGTC_2472	5377652	5.82	49.4 µM	3.60E-7	TRP & mitochondrial translation	22	3.00E-9
11	SGTC_1078	idebenone	5.78	36.1 µM	4.15E-7	Golgi	14	3.63E-9
12	SGTC_528	3492-0019	5.48	172.0 µM	1.52E-6	calcium & mitochondrial duress	140	2.10E-8
13	SGTC_903	1134-0042	5.47	114.0 µM	1.60E-6		7	2.25E-8
14	SGTC_2413	inermin	5.40	26.1 µM	2.11E-6		25	3.25E-8
15	SGTC_2737	bleomycin	5.30	30.5 nM	3.29E-6	Golgi	40	5.93E-8
16	SGTC_3202	9113761	5.06	49.5 µM	8.47E-6		21	2.12E-7
17	SGTC_498	2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)	4.96	150.0 µM	1.22E-5	Golgi	27	3.47E-7
18	SGTC_269	exalamide	4.96	29.0 µM	1.23E-5	ergosterol biosynthesis	7	3.50E-7
19	SGTC_65	0798-0905	4.93	35.0 µM	1.40E-5	calcium & mitochondrial duress	54	4.17E-7
20	SGTC_2795	7780010	4.80	54.5 µM	2.29E-5		11	8.13E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.299	2.80E-70	YOR067C	ALG8	hom	Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.290	7.43E-66	YDL096C_d	OPI6_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.262	8.54E-54	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.224	2.02E-39	YKL096C-B_p	YKL096C-B_p	hom	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.223	6.25E-39	YBL082C	ALG3	hom	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.208	4.32E-34	YGR166W	TRS65	hom	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.206	1.61E-33	YML115C	VAN1	hom	Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.201	4.67E-32	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.201	5.28E-32	YNL219C	ALG9	hom	Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.201	9.05E-32	YAL058W	CNE1	hom	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.194	7.77E-30	YJR075W	HOC1	hom	Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.174	2.75E-24	YBR229C	ROT2	hom	Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.171	1.59E-23	YBL083C_d	YBL083C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.171	2.43E-23	YAL053W	FLC2	hom	Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.167	1.85E-22	YDL095W	PMT1	hom	Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen