YOR006C / TSR3

Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR006C / TSR3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:4.69|P-value:1.25E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:4.51|P-value:2.52E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.45|P-value:9.78E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.19|P-value:1.55E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.95|P-value:1.59E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:-3.60|P-value:5.03E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.64|P-value:5.52E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-5.75|P-value:8.42E-8 ID:SGTC_551|Compound:k048-0028|FD-Score:3.50|P-value:8.39E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.81|P-value:3.16E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.94|P-value:2.02E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:5.10|P-value:2.29E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:5.06|P-value:2.73E-6 ID:SGTC_850|Compound:0388-0143|FD-Score:-3.69|P-value:3.76E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.69|P-value:4.72E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.51|P-value:8.30E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.88|P-value:2.52E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:4.82|P-value:7.38E-6 ID:SGTC_1334|Compound:1486-0610|FD-Score:5.22|P-value:1.36E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:6.17|P-value:1.39E-8 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.79|P-value:2.71E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.58|P-value:5.31E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.23|P-value:7.22E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.48|P-value:9.08E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.49|P-value:2.10E-5 ID:SGTC_1829|Compound:berberine|FD-Score:-5.18|P-value:1.20E-6 ID:SGTC_1991|Compound:st073000|FD-Score:4.14|P-value:1.01E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.63|P-value:1.61E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.76|P-value:9.40E-6 ID:SGTC_2176|Compound:5809774|FD-Score:3.54|P-value:7.52E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.35|P-value:4.65E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-3.54|P-value:6.05E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.67|P-value:4.90E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.60|P-value:5.00E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.46|P-value:9.51E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.41|P-value:9.24E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.71|P-value:4.39E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.58|P-value:6.64E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.39|P-value:6.19E-7 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:4.34|P-value:4.78E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:5.75|P-value:1.17E-7 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.87|P-value:2.61E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.72|P-value:1.32E-7 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.48|P-value:7.45E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.17|P-value:8.97E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-5.07|P-value:1.95E-6 ID:SGTC_2945|Compound:9067681|FD-Score:-4.45|P-value:2.48E-5 ID:SGTC_2950|Compound:9072895|FD-Score:4.25|P-value:6.86E-5 ID:SGTC_2954|Compound:9074818|FD-Score:4.52|P-value:2.47E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.36|P-value:4.43E-5 ID:SGTC_3069|Compound:9117009|FD-Score:3.90|P-value:2.36E-4 ID:SGTC_3072|Compound:9116419|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_3134|Compound:9127454|FD-Score:-4.28|P-value:4.82E-5 ID:SGTC_3141|Compound:9094609|FD-Score:-4.15|P-value:7.71E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.59|P-value:6.32E-4 ID:SGTC_3283|Compound:9109332|FD-Score:4.68|P-value:1.31E-5 ID:SGTC_3296|Compound:9122258|FD-Score:-3.71|P-value:3.54E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:4.69|P-value:1.25E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:4.51|P-value:2.52E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.45|P-value:9.78E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.19|P-value:1.55E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.95|P-value:1.59E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:-3.60|P-value:5.03E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.64|P-value:5.52E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-5.75|P-value:8.42E-8 ID:SGTC_551|Compound:k048-0028|FD-Score:3.50|P-value:8.39E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.81|P-value:3.16E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.94|P-value:2.02E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:5.10|P-value:2.29E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:5.06|P-value:2.73E-6 ID:SGTC_850|Compound:0388-0143|FD-Score:-3.69|P-value:3.76E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.69|P-value:4.72E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.51|P-value:8.30E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.88|P-value:2.52E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:4.82|P-value:7.38E-6 ID:SGTC_1334|Compound:1486-0610|FD-Score:5.22|P-value:1.36E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:6.17|P-value:1.39E-8 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.79|P-value:2.71E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.58|P-value:5.31E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.23|P-value:7.22E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.48|P-value:9.08E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.49|P-value:2.10E-5 ID:SGTC_1829|Compound:berberine|FD-Score:-5.18|P-value:1.20E-6 ID:SGTC_1991|Compound:st073000|FD-Score:4.14|P-value:1.01E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.63|P-value:1.61E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.76|P-value:9.40E-6 ID:SGTC_2176|Compound:5809774|FD-Score:3.54|P-value:7.52E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.35|P-value:4.65E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-3.54|P-value:6.05E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.67|P-value:4.90E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.60|P-value:5.00E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.46|P-value:9.51E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.41|P-value:9.24E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.71|P-value:4.39E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.58|P-value:6.64E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.39|P-value:6.19E-7 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:4.34|P-value:4.78E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:5.75|P-value:1.17E-7 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.87|P-value:2.61E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.72|P-value:1.32E-7 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.48|P-value:7.45E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.17|P-value:8.97E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-5.07|P-value:1.95E-6 ID:SGTC_2945|Compound:9067681|FD-Score:-4.45|P-value:2.48E-5 ID:SGTC_2950|Compound:9072895|FD-Score:4.25|P-value:6.86E-5 ID:SGTC_2954|Compound:9074818|FD-Score:4.52|P-value:2.47E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.36|P-value:4.43E-5 ID:SGTC_3069|Compound:9117009|FD-Score:3.90|P-value:2.36E-4 ID:SGTC_3072|Compound:9116419|FD-Score:4.68|P-value:1.29E-5 ID:SGTC_3134|Compound:9127454|FD-Score:-4.28|P-value:4.82E-5 ID:SGTC_3141|Compound:9094609|FD-Score:-4.15|P-value:7.71E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.59|P-value:6.32E-4 ID:SGTC_3283|Compound:9109332|FD-Score:4.68|P-value:1.31E-5 ID:SGTC_3296|Compound:9122258|FD-Score:-3.71|P-value:3.54E-4

Top fitness defect scores for YOR006C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1340 1486-1359 6.17 257.0 µM 1.39E-8 3 3.43E-10
2 SGTC_2627 acetyl isogambogic acid 5.75 100.0 µM 1.17E-7 TSC3-RPN4 16 4.58E-9
3 SGTC_2688 kanamycin b 5.72 96.7 µM 1.32E-7 calcium & mitochondrial duress 175 5.32E-9
4 SGTC_2445 5742597 5.39 35.6 µM 6.19E-7 15 3.51E-8
5 SGTC_1334 1486-0610 5.22 236.0 µM 1.36E-6 60S ribosome export 4 9.15E-8
6 SGTC_219 mitoxanthrone 5.19 164.4 µM 1.55E-6 DNA intercalators 25 1.08E-7
7 SGTC_718 1130-0073 5.10 349.0 µM 2.29E-6 8 1.73E-7
8 SGTC_800 3448-7362 5.06 155.0 µM 2.73E-6 translation 87 2.15E-7
9 SGTC_1325 1431-0015 4.82 227.0 µM 7.38E-6 Golgi 6 7.21E-7
10 SGTC_2011 4012611 4.76 177.0 µM 9.40E-6 mitochondrial processes 14 9.68E-7
11 SGTC_7 0192-1582 4.69 4.2 µM 1.25E-5 41 1.37E-6
12 SGTC_3072 9116419 4.68 49.5 µM 1.29E-5 ERG2 6 1.42E-6
13 SGTC_1154 4088-0362 4.68 150.0 µM 1.29E-5 8 1.43E-6
14 SGTC_3283 9109332 4.68 35.8 µM 1.31E-5 DNA intercalators 5 1.46E-6
15 SGTC_2006 4011487 4.63 200.0 µM 1.61E-5 endomembrane recycling 63 1.87E-6
16 SGTC_2954 9074818 4.52 52.7 µM 2.47E-5 24 3.14E-6
17 SGTC_61 0978-8327 4.51 98.6 µM 2.52E-5 ergosterol biosynthesis 16 3.22E-6
18 SGTC_3037 9091026 4.36 49.5 µM 4.43E-5 NEO1-PIK1 100 6.40E-6
19 SGTC_2268 7933382 4.35 153.8 µM 4.65E-5 28 6.79E-6
20 SGTC_2578 7,8-dihydroxyflavone 4.34 64.4 µM 4.78E-5 26 7.02E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 5.96E-15 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.128 1.00E-13 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.126 2.07E-13 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.125 3.63E-13 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.125 4.10E-13 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.124 6.60E-13 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.123 8.24E-13 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.120 3.70E-12 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.118 6.13E-12 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.117 1.00E-11 YOR295W UAF30 hom Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
0.116 1.36E-11 YGL043W DST1 hom General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
0.116 1.36E-11 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.116 1.48E-11 YBR181C RPS6B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
0.115 2.23E-11 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.115 2.69E-11 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene