YOR056C / NOB1

Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR056C / NOB1

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:-3.02|P-value:9.04E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:3.24|P-value:5.72E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.63|P-value:1.30E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:-3.78|P-value:4.81E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.15|P-value:5.69E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.75|P-value:8.01E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-4.45|P-value:2.36E-6 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.38|P-value:3.47E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.05|P-value:7.95E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-4.61|P-value:1.06E-6 ID:SGTC_1725|Compound:st036284|FD-Score:-4.59|P-value:1.18E-6 ID:SGTC_1727|Compound:st034309|FD-Score:5.41|P-value:2.41E-8 ID:SGTC_1739|Compound:st037196|FD-Score:-3.63|P-value:8.93E-5 ID:SGTC_1747|Compound:st037496|FD-Score:4.65|P-value:1.36E-6 ID:SGTC_1775|Compound:st081588|FD-Score:3.16|P-value:7.74E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.40|P-value:3.16E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.66|P-value:1.18E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.64|P-value:1.26E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.37|P-value:3.63E-4 ID:SGTC_2479|Compound:5617353|FD-Score:-3.32|P-value:3.05E-4 ID:SGTC_2482|Compound:5981709|FD-Score:4.07|P-value:2.11E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.94|P-value:3.73E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.16|P-value:7.78E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.05|P-value:8.12E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.94|P-value:3.77E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.25|P-value:5.47E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-4.91|P-value:2.21E-7 ID:SGTC_2944|Compound:9067346|FD-Score:5.87|P-value:1.53E-9 ID:SGTC_2965|Compound:9082772|FD-Score:-3.49|P-value:1.59E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.24|P-value:9.94E-6 ID:SGTC_3151|Compound:9099821|FD-Score:3.46|P-value:2.61E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.12|P-value:1.22E-7 ID:SGTC_3310|Compound:9130229|FD-Score:3.20|P-value:6.59E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:-3.02|P-value:9.04E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:3.24|P-value:5.72E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.63|P-value:1.30E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:-3.78|P-value:4.81E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.15|P-value:5.69E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.75|P-value:8.01E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-4.45|P-value:2.36E-6 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.38|P-value:3.47E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.05|P-value:7.95E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-4.61|P-value:1.06E-6 ID:SGTC_1725|Compound:st036284|FD-Score:-4.59|P-value:1.18E-6 ID:SGTC_1727|Compound:st034309|FD-Score:5.41|P-value:2.41E-8 ID:SGTC_1739|Compound:st037196|FD-Score:-3.63|P-value:8.93E-5 ID:SGTC_1747|Compound:st037496|FD-Score:4.65|P-value:1.36E-6 ID:SGTC_1775|Compound:st081588|FD-Score:3.16|P-value:7.74E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.40|P-value:3.16E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.66|P-value:1.18E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.64|P-value:1.26E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.37|P-value:3.63E-4 ID:SGTC_2479|Compound:5617353|FD-Score:-3.32|P-value:3.05E-4 ID:SGTC_2482|Compound:5981709|FD-Score:4.07|P-value:2.11E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.94|P-value:3.73E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.16|P-value:7.78E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.05|P-value:8.12E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.94|P-value:3.77E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.25|P-value:5.47E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-4.91|P-value:2.21E-7 ID:SGTC_2944|Compound:9067346|FD-Score:5.87|P-value:1.53E-9 ID:SGTC_2965|Compound:9082772|FD-Score:-3.49|P-value:1.59E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.24|P-value:9.94E-6 ID:SGTC_3151|Compound:9099821|FD-Score:3.46|P-value:2.61E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.12|P-value:1.22E-7 ID:SGTC_3310|Compound:9130229|FD-Score:3.20|P-value:6.59E-4

Top fitness defect scores for YOR056C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2944 9067346 5.87 25.6 µM 1.53E-9 4 2.17E-9 0.88
2 SGTC_1727 st034309 5.41 9.9 µM 2.41E-8 6 3.19E-8 0.45
3 SGTC_3217 9129673 5.12 49.5 µM 1.22E-7 4 1.54E-7 0.31
4 SGTC_1747 st037496 4.65 66.6 µM 1.36E-6 4 1.62E-6 0.67
5 SGTC_2981 9013952 4.24 39.0 µM 9.94E-6 6 1.13E-5 0.17
6 SGTC_2482 5981709 4.07 142.7 µM 2.11E-5 PDR1 14 2.35E-5 0.28
7 SGTC_2484 5809818 3.94 12.0 µM 3.73E-5 3 4.11E-5 0.25
8 SGTC_2894 9048625 3.94 32.5 µM 3.77E-5 9 4.15E-5 0.04
9 SGTC_573 3702-0553 3.75 84.7 µM 8.01E-5 4 8.67E-5 0.10
10 SGTC_2317 5986382 3.66 200.0 µM 1.18E-4 6 1.26E-4 0.07
11 SGTC_2319 6033012 3.64 152.6 µM 1.26E-4 tubulin folding & SWR complex 6 1.35E-4 0.22
12 SGTC_229 benomyl 3.63 22.9 µM 1.30E-4 tubulin folding & SWR complex 15 1.40E-4 0.01
13 SGTC_3151 9099821 3.46 49.5 µM 2.61E-4 5 2.75E-4 0.13
14 SGTC_2006 4011487 3.40 200.0 µM 3.16E-4 endomembrane recycling 26 3.32E-4 0.02
15 SGTC_1015 4130-4525 3.38 82.0 µM 3.47E-4 60S ribosome export 14 3.64E-4 0.05
16 SGTC_2383 9036125 3.37 200.0 µM 3.63E-4 4 3.80E-4 0.19
17 SGTC_2907 7721985 3.25 69.9 µM 5.47E-4 14 5.68E-4 0.16
18 SGTC_82 0054-0107 3.24 293.8 µM 5.72E-4 8 5.93E-4 0.20
19 SGTC_3310 9130229 3.20 44.1 µM 6.59E-4 Golgi 9 6.81E-4 0.02
20 SGTC_1775 st081588 3.16 35.2 µM 7.74E-4 60S ribosome export 20 7.98E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 9.73E-25 YDL220C CDC13 het Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
0.133 9.50E-15 YLR262C-A TMA7 hom Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
0.117 8.98E-12 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.115 2.64E-11 YDR021W FAL1 het Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
0.113 5.71E-11 YOR335C ALA1 het Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
0.112 7.44E-11 YML054C CYB2 hom Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
0.111 1.53E-10 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.104 1.58E-9 YBR057C MUM2 hom Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex
0.103 2.29E-9 YLR071C RGR1 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
0.102 3.04E-9 YDR236C FMN1 het Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
0.100 5.55E-9 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.099 1.08E-8 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.098 1.15E-8 YDR117C TMA64 hom Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
0.097 1.98E-8 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.095 4.04E-8 YDR143C SAN1 hom Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition