YOR074C / CDC21

Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR074C / CDC21

Click on Significant Values for Screen Details ID:SGTC_191|Compound:3914-0123|FD-Score:4.29|P-value:1.58E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:8.61|P-value:2.64E-17 ID:SGTC_635|Compound:k038-0028|FD-Score:3.60|P-value:2.38E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.13|P-value:2.61E-5 ID:SGTC_714|Compound:4296-0578|FD-Score:3.64|P-value:2.10E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.63|P-value:2.14E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.39|P-value:5.03E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.57|P-value:2.65E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.23|P-value:7.64E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.26|P-value:1.74E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.79|P-value:1.61E-6 ID:SGTC_1527|Compound:coumarin|FD-Score:3.51|P-value:3.28E-4 ID:SGTC_1554|Compound:st069299|FD-Score:5.12|P-value:3.18E-7 ID:SGTC_1755|Compound:st041960|FD-Score:4.70|P-value:2.51E-6 ID:SGTC_1778|Compound:st046819|FD-Score:3.36|P-value:5.70E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.23|P-value:8.59E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.93|P-value:5.90E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-3.94|P-value:5.56E-5 ID:SGTC_1995|Compound:st072814|FD-Score:3.32|P-value:6.51E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.31|P-value:6.63E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.50|P-value:2.97E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:5.09|P-value:3.85E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.04|P-value:3.67E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:16.00|P-value:8.25E-55 ID:SGTC_2819|Compound:7996655|FD-Score:-4.35|P-value:1.00E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-3.94|P-value:5.52E-5 ID:SGTC_2829|Compound:7992743|FD-Score:-3.66|P-value:1.65E-4 ID:SGTC_2914|Compound:7968218|FD-Score:-3.30|P-value:5.95E-4 ID:SGTC_2922|Compound:7999257|FD-Score:4.63|P-value:3.49E-6 ID:SGTC_3126|Compound:9127922|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.63|P-value:2.11E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:4.29|P-value:1.58E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:8.61|P-value:2.64E-17 ID:SGTC_635|Compound:k038-0028|FD-Score:3.60|P-value:2.38E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.13|P-value:2.61E-5 ID:SGTC_714|Compound:4296-0578|FD-Score:3.64|P-value:2.10E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.63|P-value:2.14E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.39|P-value:5.03E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.57|P-value:2.65E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.23|P-value:7.64E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.26|P-value:1.74E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.79|P-value:1.61E-6 ID:SGTC_1527|Compound:coumarin|FD-Score:3.51|P-value:3.28E-4 ID:SGTC_1554|Compound:st069299|FD-Score:5.12|P-value:3.18E-7 ID:SGTC_1755|Compound:st041960|FD-Score:4.70|P-value:2.51E-6 ID:SGTC_1778|Compound:st046819|FD-Score:3.36|P-value:5.70E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.23|P-value:8.59E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.93|P-value:5.90E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-3.94|P-value:5.56E-5 ID:SGTC_1995|Compound:st072814|FD-Score:3.32|P-value:6.51E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.31|P-value:6.63E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.50|P-value:2.97E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:5.09|P-value:3.85E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.04|P-value:3.67E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:16.00|P-value:8.25E-55 ID:SGTC_2819|Compound:7996655|FD-Score:-4.35|P-value:1.00E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-3.94|P-value:5.52E-5 ID:SGTC_2829|Compound:7992743|FD-Score:-3.66|P-value:1.65E-4 ID:SGTC_2914|Compound:7968218|FD-Score:-3.30|P-value:5.95E-4 ID:SGTC_2922|Compound:7999257|FD-Score:4.63|P-value:3.49E-6 ID:SGTC_3126|Compound:9127922|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.63|P-value:2.11E-4

Top fitness defect scores for YOR074C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2779 methotrexate 16.00 400.0 µM 8.25E-55 2 1.29E-57 9.29
2 SGTC_245 methotrexate 8.61 289.6 µM 2.64E-17 3 3.65E-18 1.43
3 SGTC_1554 st069299 5.12 38.7 µM 3.18E-7 3 1.49E-7 1.17
4 SGTC_2607 solasodine 5.09 47.4 µM 3.85E-7 6 1.82E-7 0.28
5 SGTC_1264 0783-0040 4.79 158.0 µM 1.61E-6 1 8.20E-7 0.52
6 SGTC_1755 st041960 4.70 45.2 µM 2.51E-6 6 1.31E-6 0.20
7 SGTC_2922 7999257 4.63 32.5 µM 3.49E-6 5 1.85E-6 0.69
8 SGTC_191 3914-0123 4.29 66.5 µM 1.58E-5 60S ribosome export 5 9.10E-6 0.75
9 SGTC_1259 0671-0132 4.26 43.0 µM 1.74E-5 3 1.00E-5 0.11
10 SGTC_714 4296-0578 3.64 26.1 µM 2.10E-4 1 1.38E-4 0.60
11 SGTC_3156 9099931 3.63 49.5 µM 2.11E-4 3 1.39E-4 0.15
12 SGTC_856 0433-0016 3.63 11.3 µM 2.14E-4 4 1.41E-4 0.11
13 SGTC_635 k038-0028 3.60 24.7 µM 2.38E-4 ubiquinone biosynthesis & proteasome 21 1.58E-4 0.09
14 SGTC_1147 3809-1045 3.57 12.3 µM 2.65E-4 6 1.77E-4 0.08
15 SGTC_1527 coumarin 3.51 137.0 µM 3.28E-4 10 2.21E-4 0.09
16 SGTC_3126 9127922 3.42 49.5 µM 4.62E-4 fatty acid desaturase (OLE1) 41 3.17E-4 0.08
17 SGTC_982 1491-0518 3.39 27.5 µM 5.03E-4 8 3.47E-4 0.12
18 SGTC_1778 st046819 3.36 58.9 µM 5.70E-4 11 3.96E-4 0.02
19 SGTC_1995 st072814 3.32 61.1 µM 6.51E-4 4 4.56E-4 0.08
20 SGTC_2394 5221648 3.31 200.0 µM 6.63E-4 12 4.65E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.509 6.94E-221 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.502 7.71E-214 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.216 1.21E-36 YNL256W FOL1 het Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
0.211 3.94E-35 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.211 4.28E-35 YER056C FCY2 hom Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
0.204 7.48E-33 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.189 2.48E-28 YIR039C YPS6 hom Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance
0.183 1.23E-26 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.173 6.62E-24 YBR252W DUT1 het deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
0.167 1.79E-22 YDR399W HPT1 hom Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
0.151 1.36E-18 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.150 1.83E-18 YLR385C SWC7 hom Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin
0.145 2.59E-17 YLL047W_d YLL047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
0.132 1.35E-14 YGL124C MON1 hom Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
0.127 1.62E-13 YER123W YCK3 hom Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway