YOR076C / SKI7

Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR076C / SKI7

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.66|P-value:1.69E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.92|P-value:6.07E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.27|P-value:1.14E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:3.44|P-value:3.80E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.90|P-value:7.74E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:4.83|P-value:1.08E-6 ID:SGTC_565|Compound:1418-0001|FD-Score:3.27|P-value:6.81E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.66|P-value:1.67E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:5.34|P-value:7.93E-8 ID:SGTC_1010|Compound:4112-3296|FD-Score:6.61|P-value:4.19E-11 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.62|P-value:1.65E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.57|P-value:2.37E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.38|P-value:4.63E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.47|P-value:5.73E-6 ID:SGTC_1591|Compound:genipin|FD-Score:4.76|P-value:1.48E-6 ID:SGTC_1720|Compound:st037298|FD-Score:7.39|P-value:1.88E-13 ID:SGTC_1735|Compound:st034310|FD-Score:4.20|P-value:1.91E-5 ID:SGTC_1738|Compound:st035555|FD-Score:3.63|P-value:1.86E-4 ID:SGTC_1749|Compound:st037932|FD-Score:3.30|P-value:6.20E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.29|P-value:6.39E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.44|P-value:3.76E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.23|P-value:7.89E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-4.00|P-value:3.70E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-3.16|P-value:8.42E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.27|P-value:6.80E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_2294|Compound:7971431|FD-Score:-3.13|P-value:9.41E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.64|P-value:1.80E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.58|P-value:2.25E-4 ID:SGTC_2412|Compound:st077135|FD-Score:6.94|P-value:4.59E-12 ID:SGTC_2415|Compound:st077333|FD-Score:4.35|P-value:9.96E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.55|P-value:2.53E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.26|P-value:7.08E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.27|P-value:6.89E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.40|P-value:4.40E-4 ID:SGTC_2947|Compound:9072639|FD-Score:8.24|P-value:2.64E-16 ID:SGTC_3254|Compound:9136651|FD-Score:3.34|P-value:5.45E-4 ID:SGTC_3271|Compound:9137798|FD-Score:4.54|P-value:4.14E-6 ID:SGTC_3|Compound:0833-0587|FD-Score:3.66|P-value:1.69E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.92|P-value:6.07E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.27|P-value:1.14E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:3.44|P-value:3.80E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.90|P-value:7.74E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:4.83|P-value:1.08E-6 ID:SGTC_565|Compound:1418-0001|FD-Score:3.27|P-value:6.81E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.66|P-value:1.67E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:5.34|P-value:7.93E-8 ID:SGTC_1010|Compound:4112-3296|FD-Score:6.61|P-value:4.19E-11 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.62|P-value:1.65E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.57|P-value:2.37E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.38|P-value:4.63E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.47|P-value:5.73E-6 ID:SGTC_1591|Compound:genipin|FD-Score:4.76|P-value:1.48E-6 ID:SGTC_1720|Compound:st037298|FD-Score:7.39|P-value:1.88E-13 ID:SGTC_1735|Compound:st034310|FD-Score:4.20|P-value:1.91E-5 ID:SGTC_1738|Compound:st035555|FD-Score:3.63|P-value:1.86E-4 ID:SGTC_1749|Compound:st037932|FD-Score:3.30|P-value:6.20E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.29|P-value:6.39E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.44|P-value:3.76E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.23|P-value:7.89E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-4.00|P-value:3.70E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-3.16|P-value:8.42E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.27|P-value:6.80E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_2294|Compound:7971431|FD-Score:-3.13|P-value:9.41E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.64|P-value:1.80E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.58|P-value:2.25E-4 ID:SGTC_2412|Compound:st077135|FD-Score:6.94|P-value:4.59E-12 ID:SGTC_2415|Compound:st077333|FD-Score:4.35|P-value:9.96E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.55|P-value:2.53E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.26|P-value:7.08E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.27|P-value:6.89E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.40|P-value:4.40E-4 ID:SGTC_2947|Compound:9072639|FD-Score:8.24|P-value:2.64E-16 ID:SGTC_3254|Compound:9136651|FD-Score:3.34|P-value:5.45E-4 ID:SGTC_3271|Compound:9137798|FD-Score:4.54|P-value:4.14E-6

Top fitness defect scores for YOR076C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2947 9072639 8.24 7.7 µM 2.64E-16 tubulin folding & SWR complex 7 8.32E-17
2 SGTC_1720 st037298 7.39 9.5 µM 1.88E-13 5 7.28E-14
3 SGTC_2412 st077135 6.94 33.4 µM 4.59E-12 3 1.97E-12
4 SGTC_1010 4112-3296 6.61 15.2 µM 4.19E-11 3 1.93E-11
5 SGTC_975 1335-0046 5.34 28.5 µM 7.93E-8 RPP1 & pyrimidine depletion 12 4.65E-8
6 SGTC_115 0527-0192 5.27 112.0 µM 1.14E-7 25 6.75E-8
7 SGTC_145 3737-0113 4.90 38.4 µM 7.74E-7 DNA intercalators 48 4.88E-7
8 SGTC_522 1273-0010 4.83 11.6 µM 1.08E-6 60S ribosome export 12 6.90E-7
9 SGTC_1591 genipin 4.76 88.4 µM 1.48E-6 mitochondrial processes 3 9.50E-7
10 SGTC_3271 9137798 4.54 49.5 µM 4.14E-6 2 2.76E-6
11 SGTC_1529 apigenin 4.47 74.0 µM 5.73E-6 21 3.85E-6
12 SGTC_2415 st077333 4.35 57.7 µM 9.96E-6 34 6.82E-6
13 SGTC_1735 st034310 4.20 56.8 µM 1.91E-5 Golgi 20 1.34E-5
14 SGTC_8 0354-0001 3.92 70.0 µM 6.07E-5 78 4.41E-5
15 SGTC_2277 7947813 3.77 106.9 µM 1.12E-4 RSC complex & mRNA processing 30 8.32E-5
16 SGTC_568 1193-0097 3.66 7.5 µM 1.67E-4 ERAD & cell cycle 167 1.26E-4
17 SGTC_3 0833-0587 3.66 95.6 µM 1.69E-4 redox potentiating 27 1.27E-4
18 SGTC_2348 9022543 3.64 33.4 µM 1.80E-4 mitochondrial processes 36 1.35E-4
19 SGTC_1738 st035555 3.63 74.0 µM 1.86E-4 TSC3-RPN4 43 1.40E-4
20 SGTC_2401 5128683 3.58 200.0 µM 2.25E-4 53 1.71E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 6.39E-12 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.110 1.93E-10 YPL197C_d YPL197C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.107 4.47E-10 YOR190W SPR1 hom Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance
0.107 4.71E-10 YPL038W MET31 hom Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication
0.104 1.82E-9 YEL055C POL5 het DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
0.100 6.16E-9 YFR032C-A RPL29 hom Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
0.099 9.15E-9 YPR153W YPR153W hom Putative protein of unknown function
0.095 3.75E-8 YMR013C SEC59 het Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
0.092 1.03E-7 YNL306W MRPS18 het Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
0.089 2.31E-7 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.089 2.74E-7 YDR155C CPR1 hom Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress
0.088 2.95E-7 YOL128C YGK3 hom Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation
0.087 4.43E-7 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.086 5.61E-7 YLL001W DNM1 hom Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance
0.085 7.77E-7 YJL142C_d IRC9_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci