HIPHOP chemogenomics database

YOR132W / VPS17

Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR132W / VPS17

Click on Significant Values for Screen Details

Top fitness defect scores for YOR132W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_227	fluconazole	11.10	33.5 µM	2.71E-29	azole & statin	13	5.98E-29
2	SGTC_2896	9052656	7.06	71.4 µM	5.94E-13	endomembrane recycling	27	8.22E-13
3	SGTC_1844	rapamycin	7.05	4.0 nM	6.63E-13		71	9.15E-13
4	SGTC_2679	rapamycin	6.83	1.0 nM	3.18E-12		62	4.30E-12
5	SGTC_168	3970-0862	6.08	530.5 µM	4.63E-10		39	5.91E-10
6	SGTC_377	3970-0964	5.77	33.1 µM	3.27E-9		19	4.07E-9
7	SGTC_2418	pentamidine	5.70	31.7 µM	4.86E-9	mitochondrial processes	17	6.02E-9
8	SGTC_2339	9000433	5.59	200.0 µM	9.07E-9		6	1.11E-8
9	SGTC_124	0136-0217	5.43	7.1 µM	2.26E-8	mitochondrial stress	28	2.75E-8
10	SGTC_1159	4092-0538	5.12	61.2 µM	1.29E-7		9	1.54E-7
11	SGTC_528	3492-0019	4.96	172.0 µM	2.97E-7	calcium & mitochondrial duress	157	3.50E-7
12	SGTC_1942	st076330	4.64	65.2 µM	1.52E-6		16	1.75E-6
13	SGTC_432	NS 1619	4.59	61.3 µM	1.89E-6		14	2.18E-6
14	SGTC_522	1273-0010	4.46	11.6 µM	3.51E-6	60S ribosome export	15	4.01E-6
15	SGTC_701	4424-0219	4.39	185.0 µM	4.97E-6	calcium & mitochondrial duress	16	5.66E-6
16	SGTC_86	0417-1630	4.39	471.9 µM	5.03E-6	ubiquinone biosynthesis & proteasome	128	5.73E-6
17	SGTC_2786	7743617	4.34	45.5 µM	6.28E-6		18	7.13E-6
18	SGTC_339	3091-4833	4.26	71.4 µM	8.95E-6	iron homeostasis	16	1.01E-5
19	SGTC_149	3970-0822	4.18	46.1 µM	1.28E-5	tubulin folding & SWR complex	85	1.44E-5
20	SGTC_3209	9113616	4.18	49.5 µM	1.31E-5		22	1.48E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.256	2.53E-51	YFL033C	RIM15	hom	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.224	3.00E-39	YJL054W	TIM54	het	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.190	8.89E-29	YLR119W	SRN2	hom	Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.188	3.81E-28	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.184	4.38E-27	YHR036W	BRL1	het	Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.183	1.15E-26	YGL113W	SLD3	het	Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.169	6.18E-23	YPL084W	BRO1	hom	Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.167	2.15E-22	YDL119C_p	YDL119C_p	hom	Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.161	6.20E-21	YCR093W	CDC39	het	Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.160	1.32E-20	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.160	1.55E-20	YOR069W	VPS5	hom	Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
0.159	1.86E-20	YGL252C	RTG2	hom	Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
0.158	2.63E-20	YOR068C	VAM10	hom	Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
0.158	3.44E-20	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.150	2.84E-18	YJL195C_d	YJL195C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6