YOR149C / SMP3

Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR149C / SMP3

Click on Significant Values for Screen Details ID:SGTC_24|Compound:0417-1663|FD-Score:3.19|P-value:1.18E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-2.99|P-value:3.50E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.19|P-value:1.21E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.16|P-value:8.79E-7 ID:SGTC_202|Compound:3594-0513|FD-Score:3.22|P-value:1.07E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.33|P-value:6.23E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-2.89|P-value:5.20E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.74|P-value:2.27E-11 ID:SGTC_365|Compound:0121-0052|FD-Score:2.90|P-value:4.15E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.48|P-value:3.11E-5 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.25|P-value:1.11E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:5.64|P-value:4.77E-11 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.71|P-value:9.72E-6 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.81|P-value:6.13E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:2.84|P-value:5.24E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.02|P-value:3.06E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:2.91|P-value:3.94E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:2.80|P-value:6.35E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.49|P-value:2.92E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:3.64|P-value:1.44E-5 ID:SGTC_1715|Compound:st032160|FD-Score:3.10|P-value:1.76E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.36|P-value:6.63E-5 ID:SGTC_1720|Compound:st037298|FD-Score:-5.88|P-value:1.06E-11 ID:SGTC_1721|Compound:st082152|FD-Score:5.56|P-value:8.99E-11 ID:SGTC_1737|Compound:st035557|FD-Score:3.19|P-value:1.20E-4 ID:SGTC_1909|Compound:st060837|FD-Score:2.83|P-value:5.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.02|P-value:1.89E-6 ID:SGTC_2052|Compound:5265182|FD-Score:3.19|P-value:1.22E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.16|P-value:1.35E-4 ID:SGTC_2267|Compound:7931562|FD-Score:2.85|P-value:5.04E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.90|P-value:3.61E-6 ID:SGTC_2432|Compound:5630864|FD-Score:3.70|P-value:1.07E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.62|P-value:1.56E-5 ID:SGTC_2923|Compound:7992654|FD-Score:2.70|P-value:9.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.07|P-value:2.49E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.58|P-value:1.92E-5 ID:SGTC_3310|Compound:9130229|FD-Score:2.88|P-value:4.49E-4 ID:SGTC_24|Compound:0417-1663|FD-Score:3.19|P-value:1.18E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-2.99|P-value:3.50E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.19|P-value:1.21E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.16|P-value:8.79E-7 ID:SGTC_202|Compound:3594-0513|FD-Score:3.22|P-value:1.07E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.33|P-value:6.23E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-2.89|P-value:5.20E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.74|P-value:2.27E-11 ID:SGTC_365|Compound:0121-0052|FD-Score:2.90|P-value:4.15E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.48|P-value:3.11E-5 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.25|P-value:1.11E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:5.64|P-value:4.77E-11 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.71|P-value:9.72E-6 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.81|P-value:6.13E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:2.84|P-value:5.24E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.02|P-value:3.06E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:2.91|P-value:3.94E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:2.80|P-value:6.35E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.49|P-value:2.92E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:3.64|P-value:1.44E-5 ID:SGTC_1715|Compound:st032160|FD-Score:3.10|P-value:1.76E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.36|P-value:6.63E-5 ID:SGTC_1720|Compound:st037298|FD-Score:-5.88|P-value:1.06E-11 ID:SGTC_1721|Compound:st082152|FD-Score:5.56|P-value:8.99E-11 ID:SGTC_1737|Compound:st035557|FD-Score:3.19|P-value:1.20E-4 ID:SGTC_1909|Compound:st060837|FD-Score:2.83|P-value:5.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.02|P-value:1.89E-6 ID:SGTC_2052|Compound:5265182|FD-Score:3.19|P-value:1.22E-4 ID:SGTC_2150|Compound:5556613|FD-Score:3.16|P-value:1.35E-4 ID:SGTC_2267|Compound:7931562|FD-Score:2.85|P-value:5.04E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.90|P-value:3.61E-6 ID:SGTC_2432|Compound:5630864|FD-Score:3.70|P-value:1.07E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.62|P-value:1.56E-5 ID:SGTC_2923|Compound:7992654|FD-Score:2.70|P-value:9.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.07|P-value:2.49E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.58|P-value:1.92E-5 ID:SGTC_3310|Compound:9130229|FD-Score:2.88|P-value:4.49E-4

Top fitness defect scores for YOR149C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_359 0081-0058 5.74 15.9 µM 2.27E-11 60S ribosome export 5 4.81E-9 0.43
2 SGTC_1047 2043-3044 5.64 82.0 µM 4.77E-11 2 8.50E-9 0.97
3 SGTC_1721 st082152 5.56 9.3 µM 8.99E-11 60S ribosome export 4 1.38E-8 0.84
4 SGTC_143 4032-1194 4.16 15.7 µM 8.79E-7 13 1.60E-5 0.10
5 SGTC_1994 st072947 4.02 40.9 µM 1.89E-6 6 2.89E-5 0.13
6 SGTC_2406 ethylparaben 3.90 405.7 µM 3.61E-6 10 4.76E-5 0.49
7 SGTC_1157 4092-0394 3.71 27.6 µM 9.72E-6 4 1.02E-4 0.19
8 SGTC_2432 5630864 3.70 12.2 µM 1.07E-5 7 1.10E-4 0.03
9 SGTC_1570 verbenone 3.64 133.0 µM 1.44E-5 6 1.38E-4 0.32
10 SGTC_2605 digitonin 3.62 300.0 nM 1.56E-5 6 1.47E-4 0.26
11 SGTC_3137 9097352 3.58 49.5 µM 1.92E-5 10 1.72E-4 0.02
12 SGTC_1416 3966-0321 3.49 25.3 µM 2.92E-5 10 2.39E-4 0.21
13 SGTC_559 1550-0661 3.48 158.0 µM 3.11E-5 4 2.50E-4 0.34
14 SGTC_208 k072-0098 3.33 12.4 µM 6.23E-5 heme biosynthesis & mitochondrial translocase 26 4.28E-4 0.07
15 SGTC_202 3594-0513 3.22 34.8 µM 1.07E-4 PDR1 8 6.51E-4 0.03
16 SGTC_24 0417-1663 3.19 219.0 µM 1.18E-4 3 7.03E-4 0.04
17 SGTC_1737 st035557 3.19 69.7 µM 1.20E-4 12 7.12E-4 0.01
18 SGTC_47 0149-0135 3.19 24.2 µM 1.21E-4 TSC3-RPN4 11 7.13E-4 0.14
19 SGTC_2052 5265182 3.19 7.1 µM 1.22E-4 16 7.20E-4 0.02
20 SGTC_2150 5556613 3.16 200.0 µM 1.35E-4 15 7.76E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 4.03E-17 YHL032C GUT1 hom Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
0.139 5.18E-16 YGR144W THI4 hom Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.122 1.06E-12 YOR004W UTP23 het Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
0.120 2.79E-12 YGR148C RPL24B hom Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
0.118 5.80E-12 YDL017W CDC7 het DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
0.117 1.07E-11 YJL208C NUC1 hom Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.107 4.30E-10 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.106 6.25E-10 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.105 9.33E-10 YNL118C DCP2 het Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
0.103 1.96E-9 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.103 2.52E-9 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.098 1.24E-8 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.098 1.29E-8 YHL041W_d YHL041W_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.096 2.30E-8 YOR121C_d YOR121C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W
0.094 5.07E-8 YOL001W PHO80 hom Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling