YOR171C / LCB4

Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR171C / LCB4

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:2.87|P-value:9.70E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:2.99|P-value:6.27E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:6.79|P-value:1.54E-13 ID:SGTC_745|Compound:0469-0705|FD-Score:3.23|P-value:2.45E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.77|P-value:1.42E-7 ID:SGTC_766|Compound:1391-0721|FD-Score:3.51|P-value:7.65E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:-2.98|P-value:7.95E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.19|P-value:2.85E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-3.55|P-value:8.23E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.77|P-value:2.46E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.34|P-value:1.54E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.59|P-value:5.47E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.66|P-value:3.92E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.88|P-value:1.45E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.06|P-value:4.74E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.16|P-value:3.23E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.69|P-value:2.23E-7 ID:SGTC_1567|Compound:st069303|FD-Score:3.03|P-value:5.24E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.40|P-value:1.06E-6 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.98|P-value:9.15E-6 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.46|P-value:9.49E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.04|P-value:6.33E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.23|P-value:3.05E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.35|P-value:1.92E-4 ID:SGTC_2063|Compound:5246145|FD-Score:5.46|P-value:2.13E-9 ID:SGTC_2068|Compound:5226020|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.04|P-value:5.22E-4 ID:SGTC_2081|Compound:5214970|FD-Score:2.86|P-value:9.84E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.73|P-value:3.78E-5 ID:SGTC_2270|Compound:7924575|FD-Score:3.12|P-value:3.76E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-3.87|P-value:1.97E-5 ID:SGTC_2325|Compound:6158266|FD-Score:2.88|P-value:9.39E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.83|P-value:2.32E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.24|P-value:2.36E-4 ID:SGTC_2525|Compound:harmine|FD-Score:2.99|P-value:6.15E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-3.34|P-value:1.96E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.89|P-value:1.37E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-4.06|P-value:8.11E-6 ID:SGTC_2920|Compound:7982904|FD-Score:3.30|P-value:1.87E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.36|P-value:1.46E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.24|P-value:2.33E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.19|P-value:3.52E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.46|P-value:7.80E-7 ID:SGTC_2994|Compound:9053520|FD-Score:7.29|P-value:2.34E-15 ID:SGTC_3037|Compound:9091026|FD-Score:-3.05|P-value:6.04E-4 ID:SGTC_3076|Compound:9116187|FD-Score:3.04|P-value:5.09E-4 ID:SGTC_3094|Compound:9117742|FD-Score:2.91|P-value:8.23E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.20|P-value:2.78E-4 ID:SGTC_3168|Compound:9103419|FD-Score:3.71|P-value:3.20E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.88|P-value:1.44E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.53|P-value:7.16E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:2.87|P-value:9.70E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:2.99|P-value:6.27E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:6.79|P-value:1.54E-13 ID:SGTC_745|Compound:0469-0705|FD-Score:3.23|P-value:2.45E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.77|P-value:1.42E-7 ID:SGTC_766|Compound:1391-0721|FD-Score:3.51|P-value:7.65E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:-2.98|P-value:7.95E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.19|P-value:2.85E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-3.55|P-value:8.23E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.77|P-value:2.46E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.34|P-value:1.54E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.59|P-value:5.47E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.66|P-value:3.92E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.88|P-value:1.45E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.06|P-value:4.74E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.16|P-value:3.23E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.69|P-value:2.23E-7 ID:SGTC_1567|Compound:st069303|FD-Score:3.03|P-value:5.24E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.40|P-value:1.06E-6 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.98|P-value:9.15E-6 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.46|P-value:9.49E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.04|P-value:6.33E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.23|P-value:3.05E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.35|P-value:1.92E-4 ID:SGTC_2063|Compound:5246145|FD-Score:5.46|P-value:2.13E-9 ID:SGTC_2068|Compound:5226020|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.04|P-value:5.22E-4 ID:SGTC_2081|Compound:5214970|FD-Score:2.86|P-value:9.84E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.73|P-value:3.78E-5 ID:SGTC_2270|Compound:7924575|FD-Score:3.12|P-value:3.76E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-3.87|P-value:1.97E-5 ID:SGTC_2325|Compound:6158266|FD-Score:2.88|P-value:9.39E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.83|P-value:2.32E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.24|P-value:2.36E-4 ID:SGTC_2525|Compound:harmine|FD-Score:2.99|P-value:6.15E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-3.34|P-value:1.96E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.89|P-value:1.37E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-4.06|P-value:8.11E-6 ID:SGTC_2920|Compound:7982904|FD-Score:3.30|P-value:1.87E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.36|P-value:1.46E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.24|P-value:2.33E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.19|P-value:3.52E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.46|P-value:7.80E-7 ID:SGTC_2994|Compound:9053520|FD-Score:7.29|P-value:2.34E-15 ID:SGTC_3037|Compound:9091026|FD-Score:-3.05|P-value:6.04E-4 ID:SGTC_3076|Compound:9116187|FD-Score:3.04|P-value:5.09E-4 ID:SGTC_3094|Compound:9117742|FD-Score:2.91|P-value:8.23E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.20|P-value:2.78E-4 ID:SGTC_3168|Compound:9103419|FD-Score:3.71|P-value:3.20E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.88|P-value:1.44E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.53|P-value:7.16E-5

Top fitness defect scores for YOR171C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2994 9053520 7.29 71.4 µM 2.34E-15 RPP1 & pyrimidine depletion 2 1.50E-13
2 SGTC_457 grayanotoxin iii 6.79 135.0 µM 1.54E-13 16 5.80E-12
3 SGTC_2063 5246145 5.46 200.0 µM 2.13E-9 7 2.37E-8
4 SGTC_747 3448-8087 4.77 38.7 µM 1.42E-7 12 9.25E-7
5 SGTC_1564 D-Arginine 4.69 115.0 µM 2.23E-7 16 1.37E-6
6 SGTC_2993 9054257 4.46 71.4 µM 7.80E-7 fatty acid desaturase (OLE1) 29 4.08E-6
7 SGTC_1571 st070080 4.40 73.4 µM 1.06E-6 6 5.35E-6
8 SGTC_1598 3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin 3.98 19.1 µM 9.15E-6 18 3.50E-5
9 SGTC_2854 9024559 3.89 71.4 µM 1.37E-5 22 4.99E-5
10 SGTC_3205 9113483 3.88 49.5 µM 1.44E-5 32 5.21E-5
11 SGTC_1338 1486-1223 3.88 76.0 µM 1.45E-5 26 5.24E-5
12 SGTC_1032 k061-1018 3.77 98.7 µM 2.46E-5 28 8.30E-5
13 SGTC_3168 9103419 3.71 49.5 µM 3.20E-5 60S ribosome export 6 1.05E-4
14 SGTC_1247 0448-0043 3.66 5.8 µM 3.92E-5 ERG2 59 1.25E-4
15 SGTC_1210 3013-0140 3.59 750.0 nM 5.47E-5 12 1.67E-4
16 SGTC_3209 9113616 3.53 49.5 µM 7.16E-5 44 2.11E-4
17 SGTC_766 1391-0721 3.51 126.0 µM 7.65E-5 30 2.24E-4
18 SGTC_1645 Cervolide 3.46 78.0 µM 9.49E-5 12 2.70E-4
19 SGTC_2921 7974240 3.36 10.7 µM 1.46E-4 83 3.93E-4
20 SGTC_1135 dihydrocapsaicin 3.34 536.7 nM 1.54E-4 58 4.13E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.178 3.01E-25 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.136 2.59E-15 YOR047C STD1 hom Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]
0.129 6.15E-14 YJL134W LCB3 hom Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
0.127 1.58E-13 YOR170W_d YOR170W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4
0.109 2.45E-10 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.105 9.94E-10 YGR160W_d YGR160W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.104 1.28E-9 YLR190W MMR1 hom Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
0.100 6.45E-9 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.096 2.42E-8 YCL025C AGP1 hom Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
0.093 6.73E-8 YMR059W SEN15 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
0.093 7.11E-8 YDR042C_p YDR042C_p hom Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
0.092 9.65E-8 YJL208C NUC1 hom Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.092 1.07E-7 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.091 1.16E-7 YDR402C DIT2 hom N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s
0.091 1.23E-7 YNR044W AGA1 hom Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds