YOR254C / SEC63

Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR254C / SEC63

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:6.37|P-value:2.05E-11 ID:SGTC_52|Compound:0329-0193|FD-Score:4.89|P-value:2.00E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:7.19|P-value:4.49E-14 ID:SGTC_67|Compound:0335-0847|FD-Score:19.70|P-value:2.14E-93 ID:SGTC_86|Compound:0417-1630|FD-Score:17.80|P-value:4.05E-76 ID:SGTC_133|Compound:0724-0033|FD-Score:3.00|P-value:9.61E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:13.60|P-value:2.57E-45 ID:SGTC_142|Compound:0335-0849|FD-Score:19.00|P-value:1.79E-86 ID:SGTC_158|Compound:k038-0087|FD-Score:19.00|P-value:7.61E-87 ID:SGTC_165|Compound:k038-0023|FD-Score:12.50|P-value:6.58E-39 ID:SGTC_173|Compound:1273-0061|FD-Score:4.69|P-value:6.00E-7 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.34|P-value:2.51E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:7.45|P-value:5.45E-15 ID:SGTC_216|Compound:k038-0035|FD-Score:9.27|P-value:3.37E-22 ID:SGTC_245|Compound:methotrexate|FD-Score:3.89|P-value:2.84E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:3.52|P-value:1.34E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.45|P-value:1.61E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:7.40|P-value:8.27E-15 ID:SGTC_332|Compound:4112-0506|FD-Score:3.01|P-value:9.26E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:7.12|P-value:7.69E-14 ID:SGTC_367|Compound:0335-0881|FD-Score:8.03|P-value:4.12E-17 ID:SGTC_513|Compound:4245-1575|FD-Score:3.03|P-value:8.46E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.72|P-value:5.83E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:7.35|P-value:1.28E-14 ID:SGTC_730|Compound:1436-0052|FD-Score:3.10|P-value:6.73E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:11.60|P-value:1.06E-33 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.05|P-value:7.44E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-3.21|P-value:4.06E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.03|P-value:8.46E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.78|P-value:4.51E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.01|P-value:9.13E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:6.11|P-value:1.23E-10 ID:SGTC_1738|Compound:st035555|FD-Score:3.54|P-value:1.22E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-3.16|P-value:5.02E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.13|P-value:5.98E-4 ID:SGTC_1897|Compound:st060201|FD-Score:3.01|P-value:9.20E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-3.49|P-value:1.39E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.04|P-value:8.19E-4 ID:SGTC_2175|Compound:5807670|FD-Score:4.33|P-value:3.56E-6 ID:SGTC_2182|Compound:6325192|FD-Score:3.13|P-value:6.10E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.20|P-value:4.69E-4 ID:SGTC_2448|Compound:5185112|FD-Score:-3.17|P-value:4.82E-4 ID:SGTC_2459|Compound:5811157|FD-Score:3.44|P-value:1.82E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.34|P-value:2.69E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.44|P-value:1.84E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.64|P-value:8.13E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.41|P-value:1.88E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:7.40|P-value:8.11E-15 ID:SGTC_2601|Compound:securinine|FD-Score:3.46|P-value:1.70E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.03|P-value:8.52E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.17|P-value:4.85E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.98|P-value:1.92E-5 ID:SGTC_2795|Compound:7780010|FD-Score:-3.41|P-value:1.91E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.87|P-value:2.26E-7 ID:SGTC_2835|Compound:9006588|FD-Score:3.42|P-value:1.97E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.07|P-value:7.46E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.13|P-value:5.97E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.38|P-value:2.16E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-3.56|P-value:1.02E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.39|P-value:2.22E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.67|P-value:7.31E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:6.37|P-value:2.05E-11 ID:SGTC_52|Compound:0329-0193|FD-Score:4.89|P-value:2.00E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:7.19|P-value:4.49E-14 ID:SGTC_67|Compound:0335-0847|FD-Score:19.70|P-value:2.14E-93 ID:SGTC_86|Compound:0417-1630|FD-Score:17.80|P-value:4.05E-76 ID:SGTC_133|Compound:0724-0033|FD-Score:3.00|P-value:9.61E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:13.60|P-value:2.57E-45 ID:SGTC_142|Compound:0335-0849|FD-Score:19.00|P-value:1.79E-86 ID:SGTC_158|Compound:k038-0087|FD-Score:19.00|P-value:7.61E-87 ID:SGTC_165|Compound:k038-0023|FD-Score:12.50|P-value:6.58E-39 ID:SGTC_173|Compound:1273-0061|FD-Score:4.69|P-value:6.00E-7 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.34|P-value:2.51E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:7.45|P-value:5.45E-15 ID:SGTC_216|Compound:k038-0035|FD-Score:9.27|P-value:3.37E-22 ID:SGTC_245|Compound:methotrexate|FD-Score:3.89|P-value:2.84E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:3.52|P-value:1.34E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.45|P-value:1.61E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:7.40|P-value:8.27E-15 ID:SGTC_332|Compound:4112-0506|FD-Score:3.01|P-value:9.26E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:7.12|P-value:7.69E-14 ID:SGTC_367|Compound:0335-0881|FD-Score:8.03|P-value:4.12E-17 ID:SGTC_513|Compound:4245-1575|FD-Score:3.03|P-value:8.46E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.72|P-value:5.83E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:7.35|P-value:1.28E-14 ID:SGTC_730|Compound:1436-0052|FD-Score:3.10|P-value:6.73E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:11.60|P-value:1.06E-33 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.05|P-value:7.44E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-3.21|P-value:4.06E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.03|P-value:8.46E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.78|P-value:4.51E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.01|P-value:9.13E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:6.11|P-value:1.23E-10 ID:SGTC_1738|Compound:st035555|FD-Score:3.54|P-value:1.22E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-3.16|P-value:5.02E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.13|P-value:5.98E-4 ID:SGTC_1897|Compound:st060201|FD-Score:3.01|P-value:9.20E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-3.49|P-value:1.39E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.04|P-value:8.19E-4 ID:SGTC_2175|Compound:5807670|FD-Score:4.33|P-value:3.56E-6 ID:SGTC_2182|Compound:6325192|FD-Score:3.13|P-value:6.10E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.20|P-value:4.69E-4 ID:SGTC_2448|Compound:5185112|FD-Score:-3.17|P-value:4.82E-4 ID:SGTC_2459|Compound:5811157|FD-Score:3.44|P-value:1.82E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.34|P-value:2.69E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.44|P-value:1.84E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.64|P-value:8.13E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.41|P-value:1.88E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:7.40|P-value:8.11E-15 ID:SGTC_2601|Compound:securinine|FD-Score:3.46|P-value:1.70E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.03|P-value:8.52E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.17|P-value:4.85E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.98|P-value:1.92E-5 ID:SGTC_2795|Compound:7780010|FD-Score:-3.41|P-value:1.91E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.87|P-value:2.26E-7 ID:SGTC_2835|Compound:9006588|FD-Score:3.42|P-value:1.97E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.07|P-value:7.46E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.13|P-value:5.97E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.38|P-value:2.16E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-3.56|P-value:1.02E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.39|P-value:2.22E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.67|P-value:7.31E-5

Top fitness defect scores for YOR254C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_67 0335-0847 19.70 266.0 µM 2.14E-93 ubiquinone biosynthesis & proteasome 1 7.48E-87 7.59
2 SGTC_158 k038-0087 19.00 26.4 µM 7.61E-87 ubiquinone biosynthesis & proteasome 1 8.98E-81 4.87
3 SGTC_142 0335-0849 19.00 62.7 µM 1.79E-86 ubiquinone biosynthesis & proteasome 2 1.99E-80 3.93
4 SGTC_86 0417-1630 17.80 471.9 µM 4.05E-76 ubiquinone biosynthesis & proteasome 1 8.06E-71 4.61
5 SGTC_137 0438-0332 13.60 85.4 µM 2.57E-45 ubiquinone biosynthesis & proteasome 6 3.12E-42 2.71
6 SGTC_165 k038-0023 12.50 64.4 µM 6.58E-39 ubiquinone biosynthesis & proteasome 1 2.78E-36 3.19
7 SGTC_794 k038-0031 11.60 316.0 µM 1.06E-33 ubiquinone biosynthesis & proteasome 2 1.89E-31 2.26
8 SGTC_216 k038-0035 9.27 28.4 µM 3.37E-22 ubiquinone biosynthesis & proteasome 3 9.14E-21 3.26
9 SGTC_367 0335-0881 8.03 7.1 µM 4.12E-17 ubiquinone biosynthesis & proteasome 7 4.87E-16 2.24
10 SGTC_200 k038-0079 7.45 37.5 µM 5.45E-15 ubiquinone biosynthesis & proteasome 3 4.57E-14 0.91
11 SGTC_2593 nobiletin 7.40 86.2 µM 8.11E-15 1 6.62E-14 1.17
12 SGTC_294 k038-0071 7.40 157.0 µM 8.27E-15 ubiquinone biosynthesis & proteasome 4 6.73E-14 0.32
13 SGTC_635 k038-0028 7.35 24.7 µM 1.28E-14 ubiquinone biosynthesis & proteasome 4 1.01E-13 1.09
14 SGTC_64 000l-0018 7.19 272.1 µM 4.49E-14 ubiquinone biosynthesis & proteasome 24 3.24E-13 0.42
15 SGTC_340 k038-0059 7.12 454.0 µM 7.69E-14 ubiquinone biosynthesis & proteasome 3 5.36E-13 1.79
16 SGTC_36 0335-0881 6.37 14.2 µM 2.05E-11 ubiquinone biosynthesis & proteasome 13 9.65E-11 0.31
17 SGTC_1511 0906-3109 6.11 28.5 µM 1.23E-10 excess fatty acid 2 5.12E-10 0.89
18 SGTC_52 0329-0193 4.89 875.0 nM 2.00E-7 TSC3-RPN4 4 4.98E-7 0.17
19 SGTC_2797 7859098 4.87 81.8 µM 2.26E-7 3 5.58E-7 0.15
20 SGTC_173 1273-0061 4.69 2.7 µM 6.00E-7 mitochondrial stress 14 1.39E-6 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.679 0 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.665 0 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.631 0 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.628 0 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.615 0 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.601 0 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.593 2.36E-317 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.585 7.04E-307 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.581 4.03E-302 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.571 6.71E-290 YPR065W ROX1 hom Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.562 1.98E-279 YLR378C SEC61 het Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
0.557 1.85E-273 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.555 4.27E-271 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.548 1.14E-261 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.547 3.14E-261 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data