YOR265W / RBL2

Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR265W / RBL2

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:4.72|P-value:4.41E-6 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.43|P-value:5.48E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.34|P-value:8.46E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.44|P-value:5.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:4.02|P-value:7.76E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.56|P-value:8.78E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:3.33|P-value:8.58E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.76|P-value:1.75E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.26|P-value:9.44E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.66|P-value:2.91E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.36|P-value:7.86E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.68|P-value:2.68E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:5.30|P-value:3.00E-7 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-3.45|P-value:5.04E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.66|P-value:2.83E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.78|P-value:1.88E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-4.18|P-value:3.53E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.57|P-value:3.36E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.82|P-value:1.61E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_1612|Compound:st002233|FD-Score:3.31|P-value:9.20E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.36|P-value:8.04E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.50|P-value:1.11E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.81|P-value:2.99E-6 ID:SGTC_1678|Compound:st019265|FD-Score:3.85|P-value:1.44E-4 ID:SGTC_1711|Compound:st032263|FD-Score:3.70|P-value:2.46E-4 ID:SGTC_1763|Compound:st044482|FD-Score:5.47|P-value:1.24E-7 ID:SGTC_1782|Compound:st039423|FD-Score:4.18|P-value:4.22E-5 ID:SGTC_1860|Compound:st056228|FD-Score:3.46|P-value:5.68E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.47|P-value:5.51E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.74|P-value:2.20E-4 ID:SGTC_2141|Compound:5621638|FD-Score:5.13|P-value:6.82E-7 ID:SGTC_2374|Compound:9071832|FD-Score:-3.50|P-value:4.29E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.26|P-value:3.04E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.66|P-value:5.73E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.77|P-value:1.95E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.48|P-value:5.34E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-4.01|P-value:6.82E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.33|P-value:8.69E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.81|P-value:2.43E-6 ID:SGTC_3179|Compound:9107801|FD-Score:3.64|P-value:3.13E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:4.72|P-value:4.41E-6 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.43|P-value:5.48E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.34|P-value:8.46E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.44|P-value:5.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:4.02|P-value:7.76E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.56|P-value:8.78E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:3.33|P-value:8.58E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.76|P-value:1.75E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.26|P-value:9.44E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:3.66|P-value:2.91E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.36|P-value:7.86E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.68|P-value:2.68E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:5.30|P-value:3.00E-7 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-3.45|P-value:5.04E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.66|P-value:2.83E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.78|P-value:1.88E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-4.18|P-value:3.53E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.57|P-value:3.36E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.82|P-value:1.61E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_1612|Compound:st002233|FD-Score:3.31|P-value:9.20E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.36|P-value:8.04E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.50|P-value:1.11E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.81|P-value:2.99E-6 ID:SGTC_1678|Compound:st019265|FD-Score:3.85|P-value:1.44E-4 ID:SGTC_1711|Compound:st032263|FD-Score:3.70|P-value:2.46E-4 ID:SGTC_1763|Compound:st044482|FD-Score:5.47|P-value:1.24E-7 ID:SGTC_1782|Compound:st039423|FD-Score:4.18|P-value:4.22E-5 ID:SGTC_1860|Compound:st056228|FD-Score:3.46|P-value:5.68E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.47|P-value:5.51E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.74|P-value:2.20E-4 ID:SGTC_2141|Compound:5621638|FD-Score:5.13|P-value:6.82E-7 ID:SGTC_2374|Compound:9071832|FD-Score:-3.50|P-value:4.29E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.26|P-value:3.04E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.66|P-value:5.73E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.77|P-value:1.95E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.48|P-value:5.34E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-4.01|P-value:6.82E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.33|P-value:8.69E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.81|P-value:2.43E-6 ID:SGTC_3179|Compound:9107801|FD-Score:3.64|P-value:3.13E-4

Top fitness defect scores for YOR265W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1763 st044482 5.47 94.5 µM 1.24E-7 copper-dependent oxidative stress 6 2.21E-8
2 SGTC_1068 loratadine 5.30 26.9 µM 3.00E-7 7 5.93E-8
3 SGTC_2141 5621638 5.13 7.7 µM 6.82E-7 tubulin folding & SWR complex 17 1.48E-7
4 SGTC_1676 st018489 4.81 23.4 µM 2.99E-6 60S ribosome export 15 7.73E-7
5 SGTC_21 0849-0113 4.72 23.0 µM 4.41E-6 15 1.19E-6
6 SGTC_2401 5128683 4.66 200.0 µM 5.73E-6 20 1.60E-6
7 SGTC_210 3551-0445 4.56 56.8 µM 8.78E-6 49 2.57E-6
8 SGTC_1638 st008370 4.50 60.6 µM 1.11E-5 ergosterol biosynthesis 20 3.33E-6
9 SGTC_2398 5228213 4.26 48.8 µM 3.04E-5 iron homeostasis 30 1.03E-5
10 SGTC_1782 st039423 4.18 80.6 µM 4.22E-5 15 1.48E-5
11 SGTC_151 3013-0144 4.02 2.3 µM 7.76E-5 iron homeostasis 26 2.93E-5
12 SGTC_1472 k841-0093 3.95 193.0 µM 1.02E-4 17 3.96E-5
13 SGTC_1678 st019265 3.85 80.2 µM 1.44E-4 60S ribosome export 22 5.83E-5
14 SGTC_1326 1431-2094 3.82 10.8 µM 1.61E-4 tubulin folding & SWR complex 39 6.61E-5
15 SGTC_1087 pterostilbene 3.78 9.1 µM 1.88E-4 30 7.84E-5
16 SGTC_2524 avocatin b 3.77 10.6 µM 1.95E-4 27 8.17E-5
17 SGTC_2109 5483026 3.74 200.0 µM 2.20E-4 RSC complex & mRNA processing 34 9.36E-5
18 SGTC_1711 st032263 3.70 62.8 µM 2.46E-4 24 1.06E-4
19 SGTC_932 2857-0803 3.68 154.0 µM 2.68E-4 32 1.17E-4
20 SGTC_1083 didemethylchlorpromazine sulfoxide 3.66 707.4 nM 2.83E-4 redox potentiating 97 1.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 1.30E-10 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.110 1.62E-10 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.096 2.21E-8 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.096 2.36E-8 YPL171C OYE3 hom Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
0.096 2.89E-8 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.092 1.12E-7 YML007W YAP1 hom Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.083 1.52E-6 YHL030W ECM29 hom Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
0.082 2.06E-6 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.080 3.28E-6 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.080 3.41E-6 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.079 5.24E-6 YLR085C ARP6 hom Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.076 9.49E-6 YOR264W DSE3 hom Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.074 1.87E-5 YML041C VPS71 hom Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.073 2.45E-5 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.072 2.90E-5 YNR026C SEC12 het Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER