YOR274W / MOD5

Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR274W / MOD5

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.45|P-value:2.86E-10 ID:SGTC_65|Compound:0798-0905|FD-Score:3.49|P-value:3.88E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:5.89|P-value:7.04E-9 ID:SGTC_135|Compound:0933-0004|FD-Score:4.08|P-value:4.14E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:-5.51|P-value:5.82E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:10.90|P-value:4.33E-26 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.68|P-value:2.19E-8 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.81|P-value:1.89E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.33|P-value:1.53E-7 ID:SGTC_396|Compound:dithiazanine|FD-Score:-4.04|P-value:5.21E-5 ID:SGTC_406|Compound:estrone|FD-Score:5.18|P-value:2.99E-7 ID:SGTC_428|Compound:3448-4153|FD-Score:-4.02|P-value:5.71E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.41|P-value:5.32E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.97|P-value:6.51E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:1.69E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.26|P-value:8.63E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.52|P-value:3.50E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.64|P-value:2.24E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.41|P-value:1.13E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:-6.78|P-value:3.50E-11 ID:SGTC_1081|Compound:sertraline|FD-Score:3.58|P-value:2.76E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:-3.43|P-value:4.98E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-5.68|P-value:2.40E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.02|P-value:5.22E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.76|P-value:1.51E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-6.29|P-value:7.54E-10 ID:SGTC_1174|Compound:0739-0097|FD-Score:3.91|P-value:8.24E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.49|P-value:3.81E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.46|P-value:8.72E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.31|P-value:1.63E-5 ID:SGTC_1603|Compound:st002045|FD-Score:-4.18|P-value:2.91E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.52|P-value:6.74E-6 ID:SGTC_1683|Compound:st010134|FD-Score:5.14|P-value:3.72E-7 ID:SGTC_1694|Compound:st024011|FD-Score:3.88|P-value:9.21E-5 ID:SGTC_1706|Compound:st031321|FD-Score:-3.31|P-value:7.29E-4 ID:SGTC_1712|Compound:st032288|FD-Score:8.26|P-value:8.61E-16 ID:SGTC_1745|Compound:st081495|FD-Score:3.38|P-value:5.59E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.82|P-value:2.41E-14 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.59|P-value:2.72E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.52|P-value:3.62E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.69|P-value:1.86E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.52|P-value:3.42E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.39|P-value:5.35E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.82|P-value:1.16E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.24|P-value:9.22E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.39|P-value:5.41E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.86|P-value:9.88E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.31|P-value:6.98E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.79|P-value:2.05E-6 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.49|P-value:7.46E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:6.87|P-value:1.82E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:14.00|P-value:6.23E-42 ID:SGTC_2796|Compound:7949634|FD-Score:4.23|P-value:2.29E-5 ID:SGTC_2821|Compound:7996764|FD-Score:-3.40|P-value:5.44E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-5.47|P-value:7.31E-8 ID:SGTC_2878|Compound:9043973|FD-Score:-3.80|P-value:1.29E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.78|P-value:1.33E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.41|P-value:4.99E-4 ID:SGTC_3079|Compound:9117512|FD-Score:-3.32|P-value:7.16E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-4.45|P-value:9.69E-6 ID:SGTC_3087|Compound:9117406|FD-Score:-4.09|P-value:4.25E-5 ID:SGTC_3098|Compound:9119039|FD-Score:3.47|P-value:4.09E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.30|P-value:7.70E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.53|P-value:3.28E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.34|P-value:6.41E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.45|P-value:2.86E-10 ID:SGTC_65|Compound:0798-0905|FD-Score:3.49|P-value:3.88E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:5.89|P-value:7.04E-9 ID:SGTC_135|Compound:0933-0004|FD-Score:4.08|P-value:4.14E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:-5.51|P-value:5.82E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:10.90|P-value:4.33E-26 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.68|P-value:2.19E-8 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.81|P-value:1.89E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.33|P-value:1.53E-7 ID:SGTC_396|Compound:dithiazanine|FD-Score:-4.04|P-value:5.21E-5 ID:SGTC_406|Compound:estrone|FD-Score:5.18|P-value:2.99E-7 ID:SGTC_428|Compound:3448-4153|FD-Score:-4.02|P-value:5.71E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.41|P-value:5.32E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.97|P-value:6.51E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:1.69E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.26|P-value:8.63E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.52|P-value:3.50E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.64|P-value:2.24E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.41|P-value:1.13E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:-6.78|P-value:3.50E-11 ID:SGTC_1081|Compound:sertraline|FD-Score:3.58|P-value:2.76E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:-3.43|P-value:4.98E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-5.68|P-value:2.40E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.02|P-value:5.22E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.76|P-value:1.51E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-6.29|P-value:7.54E-10 ID:SGTC_1174|Compound:0739-0097|FD-Score:3.91|P-value:8.24E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.49|P-value:3.81E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.46|P-value:8.72E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.31|P-value:1.63E-5 ID:SGTC_1603|Compound:st002045|FD-Score:-4.18|P-value:2.91E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.52|P-value:6.74E-6 ID:SGTC_1683|Compound:st010134|FD-Score:5.14|P-value:3.72E-7 ID:SGTC_1694|Compound:st024011|FD-Score:3.88|P-value:9.21E-5 ID:SGTC_1706|Compound:st031321|FD-Score:-3.31|P-value:7.29E-4 ID:SGTC_1712|Compound:st032288|FD-Score:8.26|P-value:8.61E-16 ID:SGTC_1745|Compound:st081495|FD-Score:3.38|P-value:5.59E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.82|P-value:2.41E-14 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.59|P-value:2.72E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.52|P-value:3.62E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.69|P-value:1.86E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.52|P-value:3.42E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.39|P-value:5.35E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.82|P-value:1.16E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.24|P-value:9.22E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.39|P-value:5.41E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.86|P-value:9.88E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.31|P-value:6.98E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.79|P-value:2.05E-6 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.49|P-value:7.46E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:6.87|P-value:1.82E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:14.00|P-value:6.23E-42 ID:SGTC_2796|Compound:7949634|FD-Score:4.23|P-value:2.29E-5 ID:SGTC_2821|Compound:7996764|FD-Score:-3.40|P-value:5.44E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-5.47|P-value:7.31E-8 ID:SGTC_2878|Compound:9043973|FD-Score:-3.80|P-value:1.29E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.78|P-value:1.33E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.41|P-value:4.99E-4 ID:SGTC_3079|Compound:9117512|FD-Score:-3.32|P-value:7.16E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-4.45|P-value:9.69E-6 ID:SGTC_3087|Compound:9117406|FD-Score:-4.09|P-value:4.25E-5 ID:SGTC_3098|Compound:9119039|FD-Score:3.47|P-value:4.09E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.30|P-value:7.70E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.53|P-value:3.28E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.34|P-value:6.41E-4

Top fitness defect scores for YOR274W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2679 rapamycin 14.00 1.0 nM 6.23E-42 8 4.59E-45
2 SGTC_163 1327-0036 10.90 7.5 µM 4.33E-26 11 5.59E-28
3 SGTC_1712 st032288 8.26 53.4 µM 8.61E-16 calcium & mitochondrial duress 42 7.05E-17
4 SGTC_1844 rapamycin 7.82 4.0 nM 2.41E-14 60 2.55E-15
5 SGTC_2595 hexamethylquercetagetin 6.87 100.0 µM 1.82E-11 3 3.23E-12
6 SGTC_87 0100-0052 5.89 35.5 µM 7.04E-9 34 1.96E-9
7 SGTC_221 trifluoperazine 5.68 10.3 µM 2.19E-8 NEO1-PIK1 131 6.65E-9
8 SGTC_406 estrone 5.18 100.0 µM 2.99E-7 3 1.11E-7
9 SGTC_1683 st010134 5.14 710.0 nM 3.72E-7 10 1.40E-7
10 SGTC_1676 st018489 4.52 23.4 µM 6.74E-6 60S ribosome export 19 3.16E-6
11 SGTC_2592 piperlongumine 4.49 83.3 µM 7.46E-6 TSC3-RPN4 21 3.52E-6
12 SGTC_1577 3(2'-chlorophenyl)-7-ethoxycoumarin 4.46 66.5 µM 8.72E-6 11 4.17E-6
13 SGTC_1599 8-chlro-1-tetrahydronorharmanone 4.31 90.6 µM 1.63E-5 23 8.18E-6
14 SGTC_2796 7949634 4.23 45.5 µM 2.29E-5 14 1.18E-5
15 SGTC_135 0933-0004 4.08 26.4 µM 4.14E-5 60S ribosome export 20 2.22E-5
16 SGTC_1130 3454-2858 4.02 15.9 µM 5.22E-5 NEO1 88 2.85E-5
17 SGTC_445 e6 berbamine 3.97 66.0 µM 6.51E-5 NEO1-PIK1 106 3.61E-5
18 SGTC_1174 0739-0097 3.91 51.1 µM 8.24E-5 redox potentiating 35 4.65E-5
19 SGTC_1694 st024011 3.88 44.9 µM 9.21E-5 ERG2 44 5.24E-5
20 SGTC_2435 5706476 3.86 10.6 µM 9.88E-5 29 5.65E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.302 1.38E-71 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.285 8.92E-64 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.258 2.89E-52 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.247 9.50E-48 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.242 4.62E-46 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.236 8.28E-44 YBR049C REB1 het RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
0.232 2.40E-42 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.228 6.24E-41 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.216 9.96E-37 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.215 1.76E-36 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.214 4.72E-36 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.206 2.55E-33 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.206 2.17E-33 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.200 1.25E-31 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.199 3.02E-31 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway