YOR298C-A / MBF1

Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR298C-A / MBF1

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:3.13|P-value:1.61E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-2.86|P-value:5.43E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.95|P-value:3.40E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:2.92|P-value:3.84E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.53|P-value:2.43E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.12|P-value:1.79E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.13|P-value:1.72E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.46|P-value:3.48E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:-2.72|P-value:9.44E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.01|P-value:2.93E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.26|P-value:5.52E-7 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.87|P-value:5.17E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:-2.74|P-value:8.52E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.86|P-value:5.08E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.56|P-value:8.79E-8 ID:SGTC_904|Compound:1141-0088|FD-Score:-2.74|P-value:8.76E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-2.86|P-value:5.45E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.94|P-value:3.59E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:2.74|P-value:8.10E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:3.09|P-value:1.92E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:-3.06|P-value:2.32E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-10.10|P-value:2.63E-31 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.68|P-value:1.28E-5 ID:SGTC_1115|Compound:galangin|FD-Score:3.30|P-value:7.27E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.77|P-value:7.05E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:-4.08|P-value:1.53E-6 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.91|P-value:3.47E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.74|P-value:9.56E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.81|P-value:6.53E-4 ID:SGTC_1689|Compound:st020265|FD-Score:5.89|P-value:6.65E-12 ID:SGTC_1744|Compound:st037937|FD-Score:3.62|P-value:1.60E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.40|P-value:4.61E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.29|P-value:7.66E-5 ID:SGTC_2299|Compound:7976368|FD-Score:4.95|P-value:6.42E-9 ID:SGTC_2452|Compound:5863136|FD-Score:-3.35|P-value:6.24E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.03|P-value:2.42E-4 ID:SGTC_2691|Compound:st077356|FD-Score:2.92|P-value:3.93E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-2.81|P-value:6.43E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.15|P-value:1.60E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-2.92|P-value:4.15E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-4.75|P-value:2.70E-8 ID:SGTC_68|Compound:0986-0284|FD-Score:3.13|P-value:1.61E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-2.86|P-value:5.43E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.95|P-value:3.40E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:2.92|P-value:3.84E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.53|P-value:2.43E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.12|P-value:1.79E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.13|P-value:1.72E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.46|P-value:3.48E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:-2.72|P-value:9.44E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.01|P-value:2.93E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.26|P-value:5.52E-7 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.87|P-value:5.17E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:-2.74|P-value:8.52E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.86|P-value:5.08E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.56|P-value:8.79E-8 ID:SGTC_904|Compound:1141-0088|FD-Score:-2.74|P-value:8.76E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-2.86|P-value:5.45E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.94|P-value:3.59E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:2.74|P-value:8.10E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:3.09|P-value:1.92E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:-3.06|P-value:2.32E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-10.10|P-value:2.63E-31 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.68|P-value:1.28E-5 ID:SGTC_1115|Compound:galangin|FD-Score:3.30|P-value:7.27E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.77|P-value:7.05E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:-4.08|P-value:1.53E-6 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.91|P-value:3.47E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.74|P-value:9.56E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.81|P-value:6.53E-4 ID:SGTC_1689|Compound:st020265|FD-Score:5.89|P-value:6.65E-12 ID:SGTC_1744|Compound:st037937|FD-Score:3.62|P-value:1.60E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.40|P-value:4.61E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.29|P-value:7.66E-5 ID:SGTC_2299|Compound:7976368|FD-Score:4.95|P-value:6.42E-9 ID:SGTC_2452|Compound:5863136|FD-Score:-3.35|P-value:6.24E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.03|P-value:2.42E-4 ID:SGTC_2691|Compound:st077356|FD-Score:2.92|P-value:3.93E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-2.81|P-value:6.43E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.15|P-value:1.60E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-2.92|P-value:4.15E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-4.75|P-value:2.70E-8

Top fitness defect scores for YOR298C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1689 st020265 5.89 53.5 µM 6.65E-12 3 1.95E-9
2 SGTC_2299 7976368 4.95 114.9 µM 6.42E-9 15 3.73E-7
3 SGTC_1400 3771-8998 3.91 51.5 µM 3.47E-6 10 4.63E-5
4 SGTC_1744 st037937 3.62 38.9 µM 1.60E-5 40 1.49E-4
5 SGTC_376 3970-0862 3.53 59.1 µM 2.43E-5 30 2.06E-4
6 SGTC_558 k064-0027 3.46 21.3 µM 3.48E-5 DNA damage response 41 2.72E-4
7 SGTC_1745 st081495 3.40 74.0 µM 4.61E-5 RNA processing & uracil transport 105 3.38E-4
8 SGTC_1115 galangin 3.30 695.7 nM 7.27E-5 RPP1 & pyrimidine depletion 29 4.80E-4
9 SGTC_1988 st071872 3.29 53.4 µM 7.66E-5 22 4.99E-4
10 SGTC_68 0986-0284 3.13 82.8 µM 1.61E-4 122 8.86E-4
11 SGTC_988 1497-1057 3.09 103.0 µM 1.92E-4 37 0.00101
12 SGTC_2574 2',4'-dihydroxychalcone 3.03 14.6 µM 2.42E-4 56 0.00121
13 SGTC_335 4077-0256 2.95 27.6 µM 3.40E-4 53 0.00158
14 SGTC_916 4466-0038 2.94 42.0 nM 3.59E-4 cell wall 153 0.00164
15 SGTC_340 k038-0059 2.92 454.0 µM 3.84E-4 ubiquinone biosynthesis & proteasome 83 0.00173
16 SGTC_2691 st077356 2.92 60.2 µM 3.93E-4 82 0.00176
17 SGTC_1143 3454-2408 2.77 5.1 µM 7.05E-4 60S ribosome export 29 0.00277
18 SGTC_940 3232-1287 2.74 54.8 µM 8.10E-4 NEO1 103 0.00308
19 SGTC_113 0099-0221 2.65 2.5 µM 0.00112 107 0.00397
20 SGTC_1309 pyrazolanthrone 2.62 13.4 µM 0.00127 89 0.00436

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.312 6.66E-77 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.312 1.84E-76 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.283 1.24E-62 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.274 6.68E-59 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.261 2.04E-53 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.256 3.12E-51 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.255 9.51E-51 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.248 6.21E-48 YDR242W AMD2 hom Putative amidase
0.245 6.34E-47 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.241 1.25E-45 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.240 2.87E-45 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.239 8.39E-45 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.238 1.94E-44 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.235 1.86E-43 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.230 1.91E-41 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion