HIPHOP chemogenomics database

YPL017C / IRC15

Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL017C / IRC15

Click on Significant Values for Screen Details

Top fitness defect scores for YPL017C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_241	5hr heat shock (37°C) + cantharidin	11.10	155.4 µM	1.77E-23	cell wall signaling	10	6.43E-29
2	SGTC_514	4466-0038	9.57	2.6 nM	6.22E-18	cell wall	23	5.32E-22
3	SGTC_422	cantharidin	7.10	155.0 µM	1.20E-10	cell wall signaling	15	6.44E-13
4	SGTC_2584	androsterone	6.85	48.7 µM	4.75E-10	cell wall	42	3.57E-12
5	SGTC_163	1327-0036	6.55	7.5 µM	2.50E-9		38	2.82E-11
6	SGTC_2040	5100729	6.24	123.0 µM	1.27E-8		9	2.14E-10
7	SGTC_3311	9131146	6.04	2.9 µM	3.49E-8	cell wall	60	7.52E-10
8	SGTC_238	5hr heat shock (37°C) + nsc-638432	5.81	8.9 µM	1.10E-7	heat shock/prefoldin	17	3.15E-9
9	SGTC_916	4466-0038	5.65	42.0 nM	2.37E-7	cell wall	29	8.12E-9
10	SGTC_1768	st044301	5.43	37.2 µM	6.31E-7		4	2.75E-8
11	SGTC_2032	5185112	5.01	26.0 µM	4.00E-6		5	2.73E-7
12	SGTC_1148	3909-7663	4.86	27.7 µM	7.35E-6		3	5.81E-7
13	SGTC_243	5hr heat shock (37°C) + splitomicin	4.73	1.0 µM	1.26E-5	heat shock/prefoldin	29	1.14E-6
14	SGTC_3321	9137793	4.72	26.8 µM	1.28E-5	cell wall	51	1.15E-6
15	SGTC_2340	7987427	4.68	183.4 µM	1.50E-5		17	1.41E-6
16	SGTC_2912	7964298	4.66	10.9 µM	1.65E-5	cell wall	76	1.58E-6
17	SGTC_2619	obliquin	4.65	87.9 µM	1.68E-5	cell wall	108	1.62E-6
18	SGTC_239	5hr heat shock (37°C) + mitomycin C	4.64	819.4 µM	1.78E-5	heat shock/prefoldin	23	1.75E-6
19	SGTC_1631	benzylvanillin	4.62	81.9 µM	1.96E-5	Golgi	15	1.96E-6
20	SGTC_2065	5221596	4.59	34.3 µM	2.12E-5		9	2.17E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.297	1.75E-69	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.294	5.49E-68	YDR318W	MCM21	hom	Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
0.290	3.49E-66	YHR209W	CRG1	hom	S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.286	3.02E-64	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.275	1.70E-59	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.252	8.64E-50	YBL085W	BOI1	hom	Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.249	1.05E-48	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.248	2.43E-48	YMR036C	MIH1	hom	Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.237	3.04E-44	YJL095W	BCK1	hom	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.224	2.61E-39	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.223	3.37E-39	YPR120C	CLB5	hom	B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase
0.221	2.68E-38	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.213	8.24E-36	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.211	3.23E-35	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.209	4.20E-34	YHR030C	SLT2	hom	Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway