YPL064C / CWC27

Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL064C / CWC27

Click on Significant Values for Screen Details ID:SGTC_176|Compound:1486-1328|FD-Score:3.35|P-value:7.07E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.45|P-value:5.00E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:4.11|P-value:4.44E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.64|P-value:2.66E-8 ID:SGTC_435|Compound:c-PAF|FD-Score:4.68|P-value:4.04E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.39|P-value:6.24E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.05|P-value:5.63E-5 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.81|P-value:1.15E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.39|P-value:5.27E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.33|P-value:7.70E-4 ID:SGTC_1610|Compound:st001799|FD-Score:4.67|P-value:4.24E-6 ID:SGTC_1727|Compound:st034309|FD-Score:-6.36|P-value:4.34E-10 ID:SGTC_1980|Compound:st071811|FD-Score:3.36|P-value:6.85E-4 ID:SGTC_2069|Compound:5228469|FD-Score:3.45|P-value:5.16E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.66|P-value:2.40E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.55|P-value:2.96E-4 ID:SGTC_2269|Compound:7935389|FD-Score:8.60|P-value:9.17E-17 ID:SGTC_2271|Compound:7849410|FD-Score:-4.27|P-value:1.83E-5 ID:SGTC_2273|Compound:7815444|FD-Score:-3.20|P-value:9.79E-4 ID:SGTC_2283|Compound:7944793|FD-Score:6.29|P-value:8.90E-10 ID:SGTC_2395|Compound:5646831|FD-Score:3.77|P-value:1.63E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.93|P-value:1.27E-6 ID:SGTC_2452|Compound:5863136|FD-Score:3.73|P-value:1.92E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.69|P-value:2.16E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:4.84|P-value:1.89E-6 ID:SGTC_2749|Compound:bexarotene|FD-Score:5.11|P-value:5.37E-7 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.58|P-value:2.66E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.17|P-value:3.46E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.66|P-value:2.43E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.90|P-value:1.02E-4 ID:SGTC_2954|Compound:9074818|FD-Score:3.33|P-value:7.56E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.74|P-value:1.82E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.45|P-value:5.09E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.35|P-value:7.07E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.45|P-value:5.00E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:4.11|P-value:4.44E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.64|P-value:2.66E-8 ID:SGTC_435|Compound:c-PAF|FD-Score:4.68|P-value:4.04E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.39|P-value:6.24E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.05|P-value:5.63E-5 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.81|P-value:1.15E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.39|P-value:5.27E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.33|P-value:7.70E-4 ID:SGTC_1610|Compound:st001799|FD-Score:4.67|P-value:4.24E-6 ID:SGTC_1727|Compound:st034309|FD-Score:-6.36|P-value:4.34E-10 ID:SGTC_1980|Compound:st071811|FD-Score:3.36|P-value:6.85E-4 ID:SGTC_2069|Compound:5228469|FD-Score:3.45|P-value:5.16E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.66|P-value:2.40E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.55|P-value:2.96E-4 ID:SGTC_2269|Compound:7935389|FD-Score:8.60|P-value:9.17E-17 ID:SGTC_2271|Compound:7849410|FD-Score:-4.27|P-value:1.83E-5 ID:SGTC_2273|Compound:7815444|FD-Score:-3.20|P-value:9.79E-4 ID:SGTC_2283|Compound:7944793|FD-Score:6.29|P-value:8.90E-10 ID:SGTC_2395|Compound:5646831|FD-Score:3.77|P-value:1.63E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.93|P-value:1.27E-6 ID:SGTC_2452|Compound:5863136|FD-Score:3.73|P-value:1.92E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.69|P-value:2.16E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:4.84|P-value:1.89E-6 ID:SGTC_2749|Compound:bexarotene|FD-Score:5.11|P-value:5.37E-7 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.58|P-value:2.66E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.17|P-value:3.46E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.66|P-value:2.43E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.90|P-value:1.02E-4 ID:SGTC_2954|Compound:9074818|FD-Score:3.33|P-value:7.56E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.74|P-value:1.82E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.45|P-value:5.09E-4

Top fitness defect scores for YPL064C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2269 7935389 8.60 174.4 µM 9.17E-17 tubulin folding & SWR complex 2 3.87E-18
2 SGTC_2283 7944793 6.29 200.0 µM 8.90E-10 5 1.54E-10
3 SGTC_2749 bexarotene 5.11 57.4 µM 5.37E-7 8 1.63E-7
4 SGTC_2401 5128683 4.93 200.0 µM 1.27E-6 15 4.16E-7
5 SGTC_2627 acetyl isogambogic acid 4.84 100.0 µM 1.89E-6 TSC3-RPN4 28 6.39E-7
6 SGTC_435 c-PAF 4.68 6.7 µM 4.04E-6 21 1.46E-6
7 SGTC_1610 st001799 4.67 48.1 µM 4.24E-6 6 1.54E-6
8 SGTC_2766 oltipraz 4.17 83.9 µM 3.46E-5 heme requiring 22 1.51E-5
9 SGTC_422 cantharidin 4.11 155.0 µM 4.44E-5 cell wall signaling 52 1.98E-5
10 SGTC_1224 0823-0416 4.05 294.0 µM 5.63E-5 4 2.56E-5
11 SGTC_2914 7968218 3.90 45.3 µM 1.02E-4 14 4.89E-5
12 SGTC_2395 5646831 3.77 180.2 µM 1.63E-4 28 8.14E-5
13 SGTC_2997 9067075 3.74 71.4 µM 1.82E-4 unfolded protein response 59 9.18E-5
14 SGTC_2452 5863136 3.73 48.4 µM 1.92E-4 31 9.74E-5
15 SGTC_2625 euphol 3.69 100.0 µM 2.16E-4 34 1.11E-4
16 SGTC_2230 bromochlorosalicylanilide 3.66 954.5 nM 2.40E-4 7 1.24E-4
17 SGTC_2773 thioridazine 3.66 9.1 µM 2.43E-4 17 1.26E-4
18 SGTC_402 epigallocatechin gallate 3.45 10.0 µM 5.00E-4 51 2.76E-4
19 SGTC_3093 9117344 3.45 49.5 µM 5.09E-4 60S ribosome export 32 2.81E-4
20 SGTC_2069 5228469 3.45 200.0 µM 5.16E-4 27 2.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.39E-12 YNL260C LTO1 het Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.100 6.06E-9 YMR229C RRP5 het RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
0.088 3.43E-7 YLR284C ECI1 hom Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
0.086 5.48E-7 YPL173W MRPL40 hom Mitochondrial ribosomal protein of the large subunit
0.086 6.89E-7 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.085 7.57E-7 YPL152W RRD2 hom Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
0.083 1.65E-6 YHR146W CRP1 hom Protein that binds to cruciform DNA structures
0.082 2.04E-6 YCR084C TUP1 hom General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
0.079 4.23E-6 YOR096W RPS7A hom Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication
0.079 5.04E-6 YLR139C SLS1 hom Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
0.079 5.27E-6 YDL129W YDL129W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.077 7.10E-6 YHR118C ORC6 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
0.077 8.05E-6 YER065C ICL1 hom Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
0.073 2.16E-5 YML107C PML39 hom Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes
0.073 2.62E-5 YPL201C YIG1 hom Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol