YPL089C / RLM1

MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL089C / RLM1

Click on Significant Values for Screen Details ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:3.34E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:-5.49|P-value:1.07E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.88|P-value:1.12E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:-5.04|P-value:9.51E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:6.67|P-value:4.28E-10 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.67|P-value:9.74E-8 ID:SGTC_342|Compound:k048-0088|FD-Score:3.87|P-value:2.12E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:8.42|P-value:4.13E-15 ID:SGTC_360|Compound:0957-0399|FD-Score:5.66|P-value:1.01E-7 ID:SGTC_395|Compound:anisomycin|FD-Score:5.11|P-value:1.43E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.57|P-value:5.80E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:9.45|P-value:1.35E-18 ID:SGTC_422|Compound:cantharidin|FD-Score:8.46|P-value:3.03E-15 ID:SGTC_512|Compound:prima-1|FD-Score:4.44|P-value:2.51E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:3.48|P-value:7.81E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.55|P-value:3.62E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-4.29|P-value:2.35E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.91|P-value:3.43E-6 ID:SGTC_587|Compound:k089-0003|FD-Score:-4.50|P-value:1.01E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:5.82|P-value:4.65E-8 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.52|P-value:4.01E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.54|P-value:6.42E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.90|P-value:1.87E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.53|P-value:6.51E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.43|P-value:9.21E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.23|P-value:5.62E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.54|P-value:6.40E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.38|P-value:6.37E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.76|P-value:1.75E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-3.42|P-value:5.56E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.83|P-value:2.39E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.45|P-value:8.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.78|P-value:6.19E-6 ID:SGTC_2369|Compound:9071472|FD-Score:3.43|P-value:9.00E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.30|P-value:3.53E-9 ID:SGTC_2705|Compound:st078069|FD-Score:-3.27|P-value:9.07E-4 ID:SGTC_2706|Compound:st078239|FD-Score:4.13|P-value:8.25E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:3.96|P-value:1.50E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.30|P-value:8.34E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.73|P-value:3.79E-6 ID:SGTC_3098|Compound:9119039|FD-Score:3.74|P-value:3.31E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-3.28|P-value:8.79E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:3.34E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:-5.49|P-value:1.07E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.88|P-value:1.12E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:-5.04|P-value:9.51E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:6.67|P-value:4.28E-10 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.67|P-value:9.74E-8 ID:SGTC_342|Compound:k048-0088|FD-Score:3.87|P-value:2.12E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:8.42|P-value:4.13E-15 ID:SGTC_360|Compound:0957-0399|FD-Score:5.66|P-value:1.01E-7 ID:SGTC_395|Compound:anisomycin|FD-Score:5.11|P-value:1.43E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.57|P-value:5.80E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:9.45|P-value:1.35E-18 ID:SGTC_422|Compound:cantharidin|FD-Score:8.46|P-value:3.03E-15 ID:SGTC_512|Compound:prima-1|FD-Score:4.44|P-value:2.51E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:3.48|P-value:7.81E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.55|P-value:3.62E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-4.29|P-value:2.35E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.91|P-value:3.43E-6 ID:SGTC_587|Compound:k089-0003|FD-Score:-4.50|P-value:1.01E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:5.82|P-value:4.65E-8 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.52|P-value:4.01E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.54|P-value:6.42E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.90|P-value:1.87E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.53|P-value:6.51E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.43|P-value:9.21E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.23|P-value:5.62E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.54|P-value:6.40E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.38|P-value:6.37E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-3.76|P-value:1.75E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-3.42|P-value:5.56E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.83|P-value:2.39E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.45|P-value:8.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.78|P-value:6.19E-6 ID:SGTC_2369|Compound:9071472|FD-Score:3.43|P-value:9.00E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.30|P-value:3.53E-9 ID:SGTC_2705|Compound:st078069|FD-Score:-3.27|P-value:9.07E-4 ID:SGTC_2706|Compound:st078239|FD-Score:4.13|P-value:8.25E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:3.96|P-value:1.50E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.30|P-value:8.34E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.73|P-value:3.79E-6 ID:SGTC_3098|Compound:9119039|FD-Score:3.74|P-value:3.31E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-3.28|P-value:8.79E-4

Top fitness defect scores for YPL089C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_408 cantharidin 9.45 100.0 µM 1.35E-18 cell wall signaling 4 1.67E-21
2 SGTC_422 cantharidin 8.46 155.0 µM 3.03E-15 cell wall signaling 7 1.31E-17
3 SGTC_354 2783-3500 8.42 4.9 µM 4.13E-15 cell wall 10 1.88E-17
4 SGTC_241 5hr heat shock (37°C) + cantharidin 6.67 155.4 µM 4.28E-10 cell wall signaling 25 1.28E-11
5 SGTC_2489 cantharidin 6.30 73.4 µM 3.53E-9 cell wall signaling 7 1.49E-10
6 SGTC_663 1486-0733 5.82 13.9 µM 4.65E-8 cell wall signaling 21 3.00E-9
7 SGTC_255 tunicamycin 5.67 200.5 nM 9.74E-8 unfolded protein response 36 7.10E-9
8 SGTC_360 0957-0399 5.66 1.0 µM 1.01E-7 cell wall 16 7.42E-9
9 SGTC_395 anisomycin 5.11 6.2 µM 1.43E-6 3 1.62E-7
10 SGTC_586 0957-0265 4.91 8.2 µM 3.43E-6 cell wall 28 4.50E-7
11 SGTC_2368 9071271 4.78 200.0 µM 6.19E-6 13 8.96E-7
12 SGTC_512 prima-1 4.44 270.0 µM 2.51E-5 DNA intercalators 16 4.58E-6
13 SGTC_138 0850-0259 4.36 40.9 µM 3.34E-5 cell wall 172 6.39E-6
14 SGTC_2007 4011539 4.23 71.8 µM 5.62E-5 cell wall signaling 47 1.17E-5
15 SGTC_2706 st078239 4.13 82.5 µM 8.25E-5 8 1.83E-5
16 SGTC_1940 st075990 4.06 51.1 µM 1.06E-4 60S ribosome export 19 2.45E-5
17 SGTC_2756 carbadox 3.96 75.1 µM 1.50E-4 58 3.68E-5
18 SGTC_1026 k007-1105 3.90 30.6 µM 1.87E-4 ERAD & cell cycle 98 4.75E-5
19 SGTC_342 k048-0088 3.87 23.3 µM 2.12E-4 RSC & ERG11 19 5.52E-5
20 SGTC_2357 9067248 3.83 200.0 µM 2.39E-4 21 6.34E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.336 2.09E-89 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.328 9.42E-85 YJL095W BCK1 hom Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.312 7.51E-77 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.298 7.91E-70 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.293 1.15E-67 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.266 4.18E-55 YHR030C SLT2 hom Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.227 2.24E-40 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.199 3.72E-31 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.198 5.19E-31 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.169 5.89E-23 YJR018W_d YJR018W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168 9.27E-23 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.167 2.09E-22 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.164 9.79E-22 YER133W GLC7 het Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
0.161 7.54E-21 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.152 7.19E-19 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication