HIPHOP chemogenomics database

YPL101W / ELP4

Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL101W / ELP4

Click on Significant Values for Screen Details

Top fitness defect scores for YPL101W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1844	rapamycin	11.10	4.0 nM	1.25E-23		31	4.68E-29
2	SGTC_2679	rapamycin	10.50	1.0 nM	2.58E-21		23	3.58E-26
3	SGTC_697	0508-1955	8.22	564.0 µM	9.91E-14		2	9.87E-17
4	SGTC_694	1315-0438	7.86	43.2 µM	1.09E-12		1	1.95E-15
5	SGTC_258	haloperidol	5.64	50.8 µM	2.36E-7	fatty acid desaturase (OLE1)	10	8.34E-9
6	SGTC_2827	7993847	5.33	71.4 µM	9.97E-7		7	5.00E-8
7	SGTC_799	1310-0557	5.01	151.0 µM	3.95E-6	superoxide	9	2.76E-7
8	SGTC_2688	kanamycin b	4.98	96.7 µM	4.40E-6	calcium & mitochondrial duress	247	3.15E-7
9	SGTC_2582	agaric acid	4.96	100.0 µM	4.90E-6	redox potentiating	225	3.60E-7
10	SGTC_2810	7959871	4.90	45.5 µM	6.17E-6	copper-dependent oxidative stress	22	4.79E-7
11	SGTC_351	caspofungin	4.76	25.0 nM	1.08E-5	cell wall	61	9.54E-7
12	SGTC_1248	0469-0687	4.28	58.6 µM	6.67E-5		17	9.15E-6
13	SGTC_1578	josamycin	4.25	24.2 µM	7.57E-5		9	1.07E-5
14	SGTC_1147	3809-1045	4.21	12.3 µM	8.75E-5		17	1.28E-5
15	SGTC_317	4495-0002	4.21	170.0 µM	8.86E-5		7	1.30E-5
16	SGTC_2496	diffractaic acid	4.21	79.8 µM	8.86E-5		16	1.30E-5
17	SGTC_1195	1502-0147	4.20	41.1 µM	9.07E-5		19	1.34E-5
18	SGTC_2330	7979373	4.20	200.0 µM	9.19E-5	60S ribosome export	29	1.36E-5
19	SGTC_1862	st057513	4.17	3.8 µM	1.01E-4		7	1.53E-5
20	SGTC_1935	st074532	4.15	41.6 µM	1.09E-4		23	1.68E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.300	5.97E-71	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.283	6.55E-63	YLR384C	IKI3	hom	Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.278	1.54E-60	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.274	1.01E-58	YKL110C	KTI12	hom	Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.274	9.46E-59	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.258	4.69E-52	YGL211W	NCS6	hom	Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.253	5.90E-50	YHR187W	IKI1	hom	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.227	2.20E-40	YOR184W	SER1	hom	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.225	1.34E-39	YNL119W	NCS2	hom	Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.222	8.45E-39	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.221	2.05E-38	YGR200C	ELP2	hom	Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.210	7.63E-35	YMR312W	ELP6	hom	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.207	9.18E-34	YKR035W-A	DID2	hom	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.203	1.05E-32	YHR186C	KOG1	het	Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.199	2.96E-31	YHR111W	UBA4	hom	Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p