YPL143W / RPL33A

Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL143W / RPL33A

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.11|P-value:8.75E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-6.17|P-value:1.74E-10 ID:SGTC_258|Compound:haloperidol|FD-Score:3.22|P-value:5.97E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:3.17|P-value:7.10E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:-3.61|P-value:1.16E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.72|P-value:7.58E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:-4.38|P-value:4.10E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.57|P-value:1.61E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.19|P-value:6.61E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:-4.00|P-value:2.30E-5 ID:SGTC_587|Compound:k089-0003|FD-Score:3.40|P-value:3.07E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.40|P-value:2.62E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:3.15|P-value:7.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.17|P-value:7.26E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:5.23|P-value:6.56E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:4.74|P-value:8.99E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.29|P-value:3.95E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.28|P-value:4.79E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.26|P-value:5.20E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.11|P-value:7.50E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.59|P-value:1.51E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.30|P-value:4.53E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.71|P-value:9.37E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.14|P-value:1.52E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.38|P-value:3.41E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.24|P-value:5.58E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.96|P-value:2.77E-5 ID:SGTC_1700|Compound:st030840|FD-Score:3.70|P-value:9.72E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.11|P-value:7.56E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-3.10|P-value:7.84E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.16|P-value:6.28E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.53|P-value:1.59E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-3.06|P-value:9.06E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.48|P-value:1.94E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.37|P-value:2.94E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.13|P-value:1.12E-7 ID:SGTC_1967|Compound:st070177|FD-Score:3.92|P-value:3.96E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.61|P-value:1.72E-6 ID:SGTC_2064|Compound:5220758|FD-Score:3.79|P-value:6.71E-5 ID:SGTC_2276|Compound:7947513|FD-Score:3.27|P-value:5.05E-4 ID:SGTC_2393|Compound:9031399|FD-Score:4.61|P-value:1.71E-6 ID:SGTC_2413|Compound:inermin|FD-Score:-4.29|P-value:6.23E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.37|P-value:2.88E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.40|P-value:2.61E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.82|P-value:5.89E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.57|P-value:1.66E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.96|P-value:2.76E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.40|P-value:3.14E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.15|P-value:7.71E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.38|P-value:3.31E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-4.29|P-value:6.18E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-4.42|P-value:3.32E-6 ID:SGTC_3268|Compound:9137683|FD-Score:3.72|P-value:9.19E-5 ID:SGTC_3272|Compound:9137413|FD-Score:3.45|P-value:2.57E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-4.04|P-value:1.91E-5 ID:SGTC_3340|Compound:9146634|FD-Score:-3.12|P-value:7.34E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.11|P-value:8.75E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-6.17|P-value:1.74E-10 ID:SGTC_258|Compound:haloperidol|FD-Score:3.22|P-value:5.97E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:3.17|P-value:7.10E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:-3.61|P-value:1.16E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.72|P-value:7.58E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:-4.38|P-value:4.10E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.57|P-value:1.61E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.19|P-value:6.61E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:-4.00|P-value:2.30E-5 ID:SGTC_587|Compound:k089-0003|FD-Score:3.40|P-value:3.07E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.40|P-value:2.62E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:3.15|P-value:7.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.17|P-value:7.26E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.30|P-value:4.51E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:5.23|P-value:6.56E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:4.74|P-value:8.99E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.29|P-value:3.95E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.28|P-value:4.79E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.26|P-value:5.20E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.11|P-value:7.50E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:3.59|P-value:1.51E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.30|P-value:4.53E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.71|P-value:9.37E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.14|P-value:1.52E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.38|P-value:3.41E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.24|P-value:5.58E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.96|P-value:2.77E-5 ID:SGTC_1700|Compound:st030840|FD-Score:3.70|P-value:9.72E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.11|P-value:7.56E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-3.10|P-value:7.84E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.16|P-value:6.28E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-3.53|P-value:1.59E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-3.06|P-value:9.06E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.48|P-value:1.94E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.37|P-value:2.94E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.13|P-value:1.12E-7 ID:SGTC_1967|Compound:st070177|FD-Score:3.92|P-value:3.96E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.61|P-value:1.72E-6 ID:SGTC_2064|Compound:5220758|FD-Score:3.79|P-value:6.71E-5 ID:SGTC_2276|Compound:7947513|FD-Score:3.27|P-value:5.05E-4 ID:SGTC_2393|Compound:9031399|FD-Score:4.61|P-value:1.71E-6 ID:SGTC_2413|Compound:inermin|FD-Score:-4.29|P-value:6.23E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.37|P-value:2.88E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.40|P-value:2.61E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.82|P-value:5.89E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.57|P-value:1.66E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.96|P-value:2.76E-5 ID:SGTC_2993|Compound:9054257|FD-Score:3.40|P-value:3.14E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.15|P-value:7.71E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.38|P-value:3.31E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-4.29|P-value:6.18E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-4.42|P-value:3.32E-6 ID:SGTC_3268|Compound:9137683|FD-Score:3.72|P-value:9.19E-5 ID:SGTC_3272|Compound:9137413|FD-Score:3.45|P-value:2.57E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-4.04|P-value:1.91E-5 ID:SGTC_3340|Compound:9146634|FD-Score:-3.12|P-value:7.34E-4

Top fitness defect scores for YPL143W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_805 3852-0218 5.23 479.0 µM 6.56E-8 ERAD & cell cycle 6 8.39E-8 0.31
2 SGTC_1911 st060208 5.13 74.3 µM 1.12E-7 4 1.42E-7 0.15
3 SGTC_832 4083-0019 4.74 164.0 µM 8.99E-7 translation 16 1.09E-6 0.13
4 SGTC_2393 9031399 4.61 185.9 µM 1.71E-6 RNA processing & uracil transport 7 2.05E-6 0.14
5 SGTC_2050 5263056 4.61 171.0 µM 1.72E-6 fatty acid desaturase (OLE1) 17 2.05E-6 0.04
6 SGTC_1243 0416-0003 4.14 5.7 µM 1.52E-5 PDR1 3 1.74E-5 0.22
7 SGTC_1967 st070177 3.92 51.4 µM 3.96E-5 5 4.45E-5 0.08
8 SGTC_3192 9110362 3.86 49.5 µM 5.00E-5 mitochondrial processes 7 5.59E-5 0.00
9 SGTC_2825 7998094 3.82 58.4 µM 5.89E-5 fatty acid desaturase (OLE1) 13 6.57E-5 0.05
10 SGTC_2064 5220758 3.79 200.0 µM 6.71E-5 10 7.46E-5 0.01
11 SGTC_3268 9137683 3.72 49.5 µM 9.19E-5 5 1.02E-4 0.08
12 SGTC_1137 ixabepilone 3.71 100.0 µM 9.37E-5 8 1.04E-4 0.23
13 SGTC_1700 st030840 3.70 7.9 µM 9.72E-5 PDR1 9 1.07E-4 0.00
14 SGTC_975 1335-0046 3.59 28.5 µM 1.51E-4 RPP1 & pyrimidine depletion 13 1.65E-4 0.05
15 SGTC_389 tpck 3.57 16.7 µM 1.61E-4 11 1.76E-4 0.52
16 SGTC_2838 9002687 3.57 71.4 µM 1.66E-4 fatty acid desaturase (OLE1) 18 1.81E-4 0.02
17 SGTC_3272 9137413 3.45 49.5 µM 2.57E-4 13 2.79E-4 0.03
18 SGTC_587 k089-0003 3.40 57.0 µM 3.07E-4 3 3.31E-4 0.16
19 SGTC_2993 9054257 3.40 71.4 µM 3.14E-4 fatty acid desaturase (OLE1) 44 3.39E-4 2.44E-4
20 SGTC_3193 9111931 3.38 49.5 µM 3.31E-4 15 3.57E-4 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.237 4.04E-44 YPL142C_d YPL142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
0.195 5.33E-30 YPL131W RPL5 het Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
0.184 5.48E-27 YOL120C RPL18A het Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
0.168 1.35E-22 YBR190W_d YBR190W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
0.160 1.48E-20 YKL180W RPL17A het Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.158 6.29E-20 YLR076C_d YLR076C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
0.135 4.82E-15 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.132 1.58E-14 YJL111W CCT7 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
0.127 1.44E-13 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.123 8.29E-13 YLR009W RLP24 het Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
0.121 2.15E-12 YOL040C RPS15 het Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
0.120 3.50E-12 YDL143W CCT4 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.119 5.50E-12 YJR007W SUI2 het Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.118 8.68E-12 YHL015W RPS20 het Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
0.116 1.42E-11 YDR064W RPS13 het Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15