YPL183C / RTT10

WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL183C / RTT10

Click on Significant Values for Screen Details ID:SGTC_178|Compound:4092-0806|FD-Score:5.72|P-value:9.28E-9 ID:SGTC_217|Compound:ketoconazole|FD-Score:5.46|P-value:3.87E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.82|P-value:8.69E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:7.40|P-value:1.66E-13 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.48|P-value:3.20E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.09|P-value:2.77E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.75|P-value:1.37E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:5.33|P-value:7.79E-8 ID:SGTC_547|Compound:1120-0019|FD-Score:4.04|P-value:3.60E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:4.17|P-value:2.06E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:17.70|P-value:1.95E-68 ID:SGTC_659|Compound:k018-0002|FD-Score:3.50|P-value:2.95E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:14.30|P-value:1.80E-45 ID:SGTC_727|Compound:3032-0951|FD-Score:3.15|P-value:9.78E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.94|P-value:5.44E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.27|P-value:6.54E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-4.22|P-value:1.57E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:5.04|P-value:3.53E-7 ID:SGTC_955|Compound:1216-0110|FD-Score:3.99|P-value:4.42E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.43|P-value:3.80E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.46|P-value:5.89E-6 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.27|P-value:6.10E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.94|P-value:2.63E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.21|P-value:7.46E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.66|P-value:1.49E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.44|P-value:4.02E-8 ID:SGTC_1164|Compound:k060-0068|FD-Score:4.32|P-value:1.09E-5 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.27|P-value:6.64E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:7.18|P-value:7.90E-13 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.69|P-value:1.34E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.19|P-value:8.79E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.59|P-value:1.96E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.36|P-value:9.31E-6 ID:SGTC_1568|Compound:digitonin|FD-Score:3.35|P-value:4.92E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.23|P-value:7.52E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.21|P-value:8.05E-4 ID:SGTC_1721|Compound:st082152|FD-Score:3.19|P-value:8.55E-4 ID:SGTC_1800|Compound:st050079|FD-Score:7.33|P-value:2.85E-13 ID:SGTC_1803|Compound:st050068|FD-Score:3.58|P-value:2.18E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.22|P-value:7.95E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.80|P-value:9.48E-5 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.60|P-value:2.03E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.41|P-value:7.34E-6 ID:SGTC_1941|Compound:st076221|FD-Score:4.37|P-value:8.76E-6 ID:SGTC_2125|Compound:5246583|FD-Score:3.25|P-value:7.07E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.51|P-value:2.82E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2262|Compound:9014885|FD-Score:3.76|P-value:1.11E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.51|P-value:2.86E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.44|P-value:3.69E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-5.56|P-value:1.99E-8 ID:SGTC_2576|Compound:guaiol|FD-Score:6.07|P-value:1.18E-9 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.39|P-value:4.11E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-5.53|P-value:2.44E-8 ID:SGTC_2754|Compound:granisetron|FD-Score:3.29|P-value:6.09E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:4.71|P-value:1.83E-6 ID:SGTC_3306|Compound:9127676|FD-Score:4.08|P-value:3.02E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:5.72|P-value:9.28E-9 ID:SGTC_217|Compound:ketoconazole|FD-Score:5.46|P-value:3.87E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.82|P-value:8.69E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:7.40|P-value:1.66E-13 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.48|P-value:3.20E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.09|P-value:2.77E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.75|P-value:1.37E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:5.33|P-value:7.79E-8 ID:SGTC_547|Compound:1120-0019|FD-Score:4.04|P-value:3.60E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:4.17|P-value:2.06E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:17.70|P-value:1.95E-68 ID:SGTC_659|Compound:k018-0002|FD-Score:3.50|P-value:2.95E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:14.30|P-value:1.80E-45 ID:SGTC_727|Compound:3032-0951|FD-Score:3.15|P-value:9.78E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.94|P-value:5.44E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.27|P-value:6.54E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-4.22|P-value:1.57E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:5.04|P-value:3.53E-7 ID:SGTC_955|Compound:1216-0110|FD-Score:3.99|P-value:4.42E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.43|P-value:3.80E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.46|P-value:5.89E-6 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.27|P-value:6.10E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.94|P-value:2.63E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.21|P-value:7.46E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.66|P-value:1.49E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.44|P-value:4.02E-8 ID:SGTC_1164|Compound:k060-0068|FD-Score:4.32|P-value:1.09E-5 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.27|P-value:6.64E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:7.18|P-value:7.90E-13 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.69|P-value:1.34E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.19|P-value:8.79E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.59|P-value:1.96E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.36|P-value:9.31E-6 ID:SGTC_1568|Compound:digitonin|FD-Score:3.35|P-value:4.92E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.23|P-value:7.52E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.21|P-value:8.05E-4 ID:SGTC_1721|Compound:st082152|FD-Score:3.19|P-value:8.55E-4 ID:SGTC_1800|Compound:st050079|FD-Score:7.33|P-value:2.85E-13 ID:SGTC_1803|Compound:st050068|FD-Score:3.58|P-value:2.18E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.22|P-value:7.95E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.80|P-value:9.48E-5 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.60|P-value:2.03E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.41|P-value:7.34E-6 ID:SGTC_1941|Compound:st076221|FD-Score:4.37|P-value:8.76E-6 ID:SGTC_2125|Compound:5246583|FD-Score:3.25|P-value:7.07E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.51|P-value:2.82E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2262|Compound:9014885|FD-Score:3.76|P-value:1.11E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.51|P-value:2.86E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.44|P-value:3.69E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-5.56|P-value:1.99E-8 ID:SGTC_2576|Compound:guaiol|FD-Score:6.07|P-value:1.18E-9 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.39|P-value:4.11E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-5.53|P-value:2.44E-8 ID:SGTC_2754|Compound:granisetron|FD-Score:3.29|P-value:6.09E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:4.71|P-value:1.83E-6 ID:SGTC_3306|Compound:9127676|FD-Score:4.08|P-value:3.02E-5

Top fitness defect scores for YPL183C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_608 k048-0125 17.70 159.0 µM 1.95E-68 1 1.59E-70
2 SGTC_712 r015-0009 14.30 80.6 µM 1.80E-45 1 7.53E-47
3 SGTC_227 fluconazole 7.40 33.5 µM 1.66E-13 azole & statin 26 6.78E-14
4 SGTC_1800 st050079 7.33 29.1 µM 2.85E-13 NEO1-PIK1 20 1.18E-13
5 SGTC_1248 0469-0687 7.18 58.6 µM 7.90E-13 3 3.39E-13
6 SGTC_2576 guaiol 6.07 23.1 µM 1.18E-9 6 6.34E-10
7 SGTC_1031 k035-0009 5.94 16.0 µM 2.63E-9 7 1.45E-9
8 SGTC_178 4092-0806 5.72 87.5 µM 9.28E-9 ergosterol depletion effects on membrane 44 5.34E-9
9 SGTC_217 ketoconazole 5.46 989.0 nM 3.87E-8 ergosterol depletion effects on membrane 11 2.32E-8
10 SGTC_515 4092-1064 5.33 102.0 µM 7.79E-8 plasma membrane duress 31 4.79E-8
11 SGTC_358 0075-0058 5.09 9.4 µM 2.77E-7 28 1.77E-7
12 SGTC_953 1188-0045 5.04 9.7 µM 3.53E-7 60S ribosome export 8 2.27E-7
13 SGTC_2765 myclobutanil 4.71 7.7 µM 1.83E-6 azole & statin 28 1.24E-6
14 SGTC_1019 4262-0212 4.46 3.4 µM 5.89E-6 ergosterol depletion effects on membrane 42 4.14E-6
15 SGTC_1915 st067105 4.41 30.6 µM 7.34E-6 RNA pol III & RNase P/MRP 28 5.20E-6
16 SGTC_1941 st076221 4.37 61.8 µM 8.76E-6 sphingolipid biosynthesis & PDR1 61 6.24E-6
17 SGTC_1562 morin hydrate 4.36 59.1 µM 9.31E-6 11 6.64E-6
18 SGTC_1164 k060-0068 4.32 93.7 µM 1.09E-5 17 7.84E-6
19 SGTC_597 k018-0003 4.17 82.8 µM 2.06E-5 RNA pol III & RNase P/MRP 10 1.51E-5
20 SGTC_3306 9127676 4.08 59.8 µM 3.02E-5 18 2.24E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.461 8.64E-176 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.319 6.56E-80 YBR061C TRM7 hom 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
0.269 8.12E-57 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.136 2.26E-15 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.133 9.05E-15 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.133 1.35E-14 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.130 4.02E-14 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.130 4.79E-14 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.128 1.07E-13 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.126 2.71E-13 YPL126W NAN1 het U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.125 3.64E-13 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.117 1.03E-11 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.115 2.37E-11 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.113 4.71E-11 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.108 3.79E-10 YHR094C HXT1 hom Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication