YPL194W / DDC1

DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL194W / DDC1

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.44|P-value:1.38E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.09|P-value:5.71E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.32|P-value:5.58E-15 ID:SGTC_291|Compound:4130-1278|FD-Score:2.93|P-value:9.75E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.67|P-value:5.24E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:4.21|P-value:4.40E-6 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.17|P-value:4.08E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:2.99|P-value:7.99E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.46|P-value:4.29E-9 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.43|P-value:1.48E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.47|P-value:1.24E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.46|P-value:4.24E-9 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.00|P-value:7.90E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.23|P-value:3.26E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.12|P-value:4.85E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.04|P-value:6.52E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.65|P-value:5.90E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.03|P-value:6.77E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:-3.31|P-value:2.42E-4 ID:SGTC_1650|Compound:st011916|FD-Score:-2.97|P-value:8.69E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-3.03|P-value:7.04E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.14|P-value:4.65E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.84|P-value:2.50E-5 ID:SGTC_1994|Compound:st072947|FD-Score:-3.61|P-value:6.98E-5 ID:SGTC_2191|Compound:5911152|FD-Score:3.08|P-value:5.74E-4 ID:SGTC_2340|Compound:7987427|FD-Score:-3.42|P-value:1.58E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.82|P-value:2.76E-5 ID:SGTC_2344|Compound:7990960|FD-Score:-4.71|P-value:3.41E-7 ID:SGTC_2349|Compound:9023730|FD-Score:-4.39|P-value:1.85E-6 ID:SGTC_2448|Compound:5185112|FD-Score:-3.42|P-value:1.59E-4 ID:SGTC_2462|Compound:5162487|FD-Score:4.06|P-value:9.13E-6 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.01|P-value:7.52E-4 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.11|P-value:5.03E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.18|P-value:3.91E-4 ID:SGTC_3001|Compound:9076041|FD-Score:2.95|P-value:9.27E-4 ID:SGTC_3292|Compound:9120825|FD-Score:4.28|P-value:3.19E-6 ID:SGTC_3307|Compound:9126885|FD-Score:4.81|P-value:1.87E-7 ID:SGTC_38|Compound:0929-0051|FD-Score:3.44|P-value:1.38E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.09|P-value:5.71E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.32|P-value:5.58E-15 ID:SGTC_291|Compound:4130-1278|FD-Score:2.93|P-value:9.75E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.67|P-value:5.24E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:4.21|P-value:4.40E-6 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.17|P-value:4.08E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:2.99|P-value:7.99E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.46|P-value:4.29E-9 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.43|P-value:1.48E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.47|P-value:1.24E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.46|P-value:4.24E-9 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.00|P-value:7.90E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.23|P-value:3.26E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.12|P-value:4.85E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.04|P-value:6.52E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.65|P-value:5.90E-5 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.03|P-value:6.77E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:-3.31|P-value:2.42E-4 ID:SGTC_1650|Compound:st011916|FD-Score:-2.97|P-value:8.69E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-3.03|P-value:7.04E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.14|P-value:4.65E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.84|P-value:2.50E-5 ID:SGTC_1994|Compound:st072947|FD-Score:-3.61|P-value:6.98E-5 ID:SGTC_2191|Compound:5911152|FD-Score:3.08|P-value:5.74E-4 ID:SGTC_2340|Compound:7987427|FD-Score:-3.42|P-value:1.58E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.82|P-value:2.76E-5 ID:SGTC_2344|Compound:7990960|FD-Score:-4.71|P-value:3.41E-7 ID:SGTC_2349|Compound:9023730|FD-Score:-4.39|P-value:1.85E-6 ID:SGTC_2448|Compound:5185112|FD-Score:-3.42|P-value:1.59E-4 ID:SGTC_2462|Compound:5162487|FD-Score:4.06|P-value:9.13E-6 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.01|P-value:7.52E-4 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.11|P-value:5.03E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.18|P-value:3.91E-4 ID:SGTC_3001|Compound:9076041|FD-Score:2.95|P-value:9.27E-4 ID:SGTC_3292|Compound:9120825|FD-Score:4.28|P-value:3.19E-6 ID:SGTC_3307|Compound:9126885|FD-Score:4.81|P-value:1.87E-7

Top fitness defect scores for YPL194W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_268 camptothecin 7.32 39.6 µM 5.58E-15 DNA damage response 18 1.25E-13
2 SGTC_1244 0416-0015 5.46 52.5 µM 4.24E-9 ERG2 9 2.45E-8
3 SGTC_3307 9126885 4.81 69.3 µM 1.87E-7 18 7.40E-7
4 SGTC_3292 9120825 4.28 59.8 µM 3.19E-6 11 9.54E-6
5 SGTC_358 0075-0058 4.21 9.4 µM 4.40E-6 51 1.28E-5
6 SGTC_2462 5162487 4.06 171.8 µM 9.13E-6 16 2.47E-5
7 SGTC_1854 st056233 3.84 32.9 µM 2.50E-5 amide catabolism 22 6.13E-5
8 SGTC_329 k048-0121 3.67 21.3 µM 5.24E-5 RSC & ERG11 16 1.19E-4
9 SGTC_1454 4239-0054 3.65 42.6 µM 5.90E-5 45 1.33E-4
10 SGTC_1132 3456-3614 3.47 2.5 µM 1.24E-4 tubulin folding & SWR complex 79 2.61E-4
11 SGTC_38 0929-0051 3.44 58.3 µM 1.38E-4 37 2.86E-4
12 SGTC_1121 fusaric acid 3.43 630.5 nM 1.48E-4 54 3.05E-4
13 SGTC_1369 1866-0067 3.23 208.0 µM 3.26E-4 RPP1 & pyrimidine depletion 69 6.22E-4
14 SGTC_2678 equilin 3.18 100.0 µM 3.91E-4 48 7.34E-4
15 SGTC_421 streptovitacin 3.17 30.0 mM 4.08E-4 61 7.62E-4
16 SGTC_1796 st049173 3.14 110.4 µM 4.65E-4 27 8.58E-4
17 SGTC_1405 3909-7894 3.12 12.0 µM 4.85E-4 60S ribosome export 32 8.92E-4
18 SGTC_2670 beta-carotene 3.11 100.0 µM 5.03E-4 12 9.21E-4
19 SGTC_2191 5911152 3.08 200.0 µM 5.74E-4 48 0.00104
20 SGTC_1453 4237-0541 3.04 195.0 µM 6.52E-4 heme biosynthesis & mitochondrial translocase 66 0.00116

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.40E-17 YDR217C RAD9 hom DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.127 1.50E-13 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.127 1.75E-13 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.124 6.51E-13 YGL140C_p YGL140C_p hom Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains
0.119 4.43E-12 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.114 3.08E-11 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.111 1.19E-10 YOR034C AKR2 hom Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
0.108 3.15E-10 YKL222C YKL222C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
0.107 5.66E-10 YHR022C-A_p YHR022C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.104 1.66E-9 YDR180W SCC2 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
0.103 1.85E-9 YLR224W_p YLR224W_p hom F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
0.102 2.64E-9 YJR099W YUH1 hom Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
0.102 3.16E-9 YDL003W MCD1 het Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
0.101 4.60E-9 YKL221W MCH2 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.101 5.34E-9 YJL130C URA2 hom Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP