YPL195W / APL5

Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL195W / APL5

Click on Significant Values for Screen Details ID:SGTC_123|Compound:0083-0120|FD-Score:3.71|P-value:4.88E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.49|P-value:3.34E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.36|P-value:5.29E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:3.93|P-value:2.44E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:7.71|P-value:3.62E-12 ID:SGTC_263|Compound:alizarin|FD-Score:3.53|P-value:8.56E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.52|P-value:8.32E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:3.80|P-value:3.74E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:1.33E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.63|P-value:2.86E-7 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.90|P-value:2.67E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.14|P-value:1.16E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:9.81|P-value:1.39E-18 ID:SGTC_459|Compound:nigericin|FD-Score:6.22|P-value:1.64E-8 ID:SGTC_524|Compound:r073-0010|FD-Score:4.71|P-value:1.41E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:10.10|P-value:1.30E-19 ID:SGTC_679|Compound:0422-0091|FD-Score:4.56|P-value:2.60E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:3.56|P-value:7.93E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:7.60|P-value:7.21E-12 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.79|P-value:3.60E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.49|P-value:3.38E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.78|P-value:3.89E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:5.97|P-value:5.66E-8 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.33|P-value:6.06E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.38|P-value:4.99E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.72|P-value:4.83E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:5.97|P-value:5.57E-8 ID:SGTC_1555|Compound:citronellol|FD-Score:4.56|P-value:2.55E-5 ID:SGTC_1624|Compound:st003710|FD-Score:4.83|P-value:8.71E-6 ID:SGTC_1687|Compound:st020778|FD-Score:7.37|P-value:2.83E-11 ID:SGTC_1715|Compound:st032160|FD-Score:-7.55|P-value:8.44E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:3.64|P-value:6.13E-4 ID:SGTC_1828|Compound:st055811|FD-Score:4.56|P-value:2.53E-5 ID:SGTC_1840|Compound:st055363|FD-Score:-3.87|P-value:2.78E-4 ID:SGTC_1841|Compound:st054148|FD-Score:-3.74|P-value:4.29E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.52|P-value:4.64E-7 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:7.05|P-value:1.87E-10 ID:SGTC_1953|Compound:st076800|FD-Score:5.33|P-value:1.08E-6 ID:SGTC_1981|Compound:st071826|FD-Score:5.19|P-value:2.01E-6 ID:SGTC_2003|Compound:4023581|FD-Score:3.98|P-value:2.01E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-4.01|P-value:1.76E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-5.90|P-value:7.09E-8 ID:SGTC_2020|Compound:4017018|FD-Score:-5.53|P-value:4.19E-7 ID:SGTC_2026|Compound:5148700|FD-Score:3.91|P-value:2.59E-4 ID:SGTC_2030|Compound:5175110|FD-Score:3.64|P-value:6.22E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-3.69|P-value:4.99E-4 ID:SGTC_2291|Compound:7917025|FD-Score:5.94|P-value:6.59E-8 ID:SGTC_2322|Compound:7413319|FD-Score:4.79|P-value:1.06E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-3.80|P-value:3.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.01|P-value:1.72E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.59|P-value:7.08E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.47|P-value:3.64E-5 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.88|P-value:2.88E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.78|P-value:1.10E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.56|P-value:7.55E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-4.04|P-value:1.56E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.20|P-value:1.15E-27 ID:SGTC_2785|Compound:7756463|FD-Score:3.83|P-value:3.33E-4 ID:SGTC_2810|Compound:7959871|FD-Score:4.20|P-value:9.44E-5 ID:SGTC_2913|Compound:7966556|FD-Score:-4.63|P-value:1.80E-5 ID:SGTC_3216|Compound:9129716|FD-Score:4.36|P-value:5.40E-5 ID:SGTC_3217|Compound:9129673|FD-Score:6.44|P-value:5.07E-9 ID:SGTC_123|Compound:0083-0120|FD-Score:3.71|P-value:4.88E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.49|P-value:3.34E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.36|P-value:5.29E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:3.93|P-value:2.44E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:7.71|P-value:3.62E-12 ID:SGTC_263|Compound:alizarin|FD-Score:3.53|P-value:8.56E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.52|P-value:8.32E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:3.80|P-value:3.74E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:1.33E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.63|P-value:2.86E-7 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.90|P-value:2.67E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.14|P-value:1.16E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:9.81|P-value:1.39E-18 ID:SGTC_459|Compound:nigericin|FD-Score:6.22|P-value:1.64E-8 ID:SGTC_524|Compound:r073-0010|FD-Score:4.71|P-value:1.41E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:10.10|P-value:1.30E-19 ID:SGTC_679|Compound:0422-0091|FD-Score:4.56|P-value:2.60E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:3.56|P-value:7.93E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:7.60|P-value:7.21E-12 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.79|P-value:3.60E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.49|P-value:3.38E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.78|P-value:3.89E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:5.97|P-value:5.66E-8 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.33|P-value:6.06E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.38|P-value:4.99E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.72|P-value:4.83E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:5.97|P-value:5.57E-8 ID:SGTC_1555|Compound:citronellol|FD-Score:4.56|P-value:2.55E-5 ID:SGTC_1624|Compound:st003710|FD-Score:4.83|P-value:8.71E-6 ID:SGTC_1687|Compound:st020778|FD-Score:7.37|P-value:2.83E-11 ID:SGTC_1715|Compound:st032160|FD-Score:-7.55|P-value:8.44E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:3.64|P-value:6.13E-4 ID:SGTC_1828|Compound:st055811|FD-Score:4.56|P-value:2.53E-5 ID:SGTC_1840|Compound:st055363|FD-Score:-3.87|P-value:2.78E-4 ID:SGTC_1841|Compound:st054148|FD-Score:-3.74|P-value:4.29E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.52|P-value:4.64E-7 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:7.05|P-value:1.87E-10 ID:SGTC_1953|Compound:st076800|FD-Score:5.33|P-value:1.08E-6 ID:SGTC_1981|Compound:st071826|FD-Score:5.19|P-value:2.01E-6 ID:SGTC_2003|Compound:4023581|FD-Score:3.98|P-value:2.01E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-4.01|P-value:1.76E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-5.90|P-value:7.09E-8 ID:SGTC_2020|Compound:4017018|FD-Score:-5.53|P-value:4.19E-7 ID:SGTC_2026|Compound:5148700|FD-Score:3.91|P-value:2.59E-4 ID:SGTC_2030|Compound:5175110|FD-Score:3.64|P-value:6.22E-4 ID:SGTC_2260|Compound:7964312|FD-Score:-3.69|P-value:4.99E-4 ID:SGTC_2291|Compound:7917025|FD-Score:5.94|P-value:6.59E-8 ID:SGTC_2322|Compound:7413319|FD-Score:4.79|P-value:1.06E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-3.80|P-value:3.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.01|P-value:1.72E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.59|P-value:7.08E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.47|P-value:3.64E-5 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.88|P-value:2.88E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.78|P-value:1.10E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.56|P-value:7.55E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-4.04|P-value:1.56E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.20|P-value:1.15E-27 ID:SGTC_2785|Compound:7756463|FD-Score:3.83|P-value:3.33E-4 ID:SGTC_2810|Compound:7959871|FD-Score:4.20|P-value:9.44E-5 ID:SGTC_2913|Compound:7966556|FD-Score:-4.63|P-value:1.80E-5 ID:SGTC_3216|Compound:9129716|FD-Score:4.36|P-value:5.40E-5 ID:SGTC_3217|Compound:9129673|FD-Score:6.44|P-value:5.07E-9

Top fitness defect scores for YPL195W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2779 methotrexate 12.20 400.0 µM 1.15E-27 4 1.88E-34
2 SGTC_528 3492-0019 10.10 172.0 µM 1.30E-19 calcium & mitochondrial duress 39 2.67E-24
3 SGTC_445 e6 berbamine 9.81 66.0 µM 1.39E-18 NEO1-PIK1 6 5.31E-23
4 SGTC_245 methotrexate 7.71 289.6 µM 3.62E-12 4 6.39E-15
5 SGTC_841 0251-0215 7.60 91.2 µM 7.21E-12 23 1.52E-14
6 SGTC_1687 st020778 7.37 55.4 µM 2.83E-11 80 8.50E-14
7 SGTC_1951 sophoraflavanone G 7.05 9.2 µM 1.87E-10 RNA processing & uracil transport 1 9.16E-13
8 SGTC_3217 9129673 6.44 49.5 µM 5.07E-9 4 5.80E-11
9 SGTC_459 nigericin 6.22 15.3 µM 1.64E-8 28 2.54E-10
10 SGTC_1529 apigenin 5.97 74.0 µM 5.57E-8 5 1.18E-9
11 SGTC_1173 0443-0020 5.97 122.0 µM 5.66E-8 TSC3-RPN4 9 1.20E-9
12 SGTC_2291 7917025 5.94 104.3 µM 6.59E-8 iron homeostasis 21 1.45E-9
13 SGTC_391 gliotoxin 5.63 8.3 µM 2.86E-7 29 9.21E-9
14 SGTC_1873 rapamycin 5.52 2.0 nM 4.64E-7 39 1.69E-8
15 SGTC_1953 st076800 5.33 12.1 µM 1.08E-6 19 4.89E-8
16 SGTC_1981 st071826 5.19 47.0 µM 2.01E-6 4 1.06E-7
17 SGTC_1624 st003710 4.83 6.2 µM 8.71E-6 calcium & mitochondrial duress 25 6.66E-7
18 SGTC_2322 7413319 4.79 38.2 µM 1.06E-5 ERAD & cell cycle 107 8.49E-7
19 SGTC_2737 bleomycin 4.78 30.5 nM 1.10E-5 Golgi 64 8.92E-7
20 SGTC_524 r073-0010 4.71 27.8 µM 1.41E-5 34 1.21E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.419 8.06E-143 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.385 6.14E-119 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.367 1.76E-107 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.364 1.68E-105 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.359 8.18E-103 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.311 3.07E-76 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.276 7.50E-60 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.271 1.70E-57 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.245 3.58E-47 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.232 2.15E-42 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.227 1.33E-40 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.226 3.10E-40 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.225 6.98E-40 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.211 3.94E-35 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.210 1.05E-34 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules