YPL203W / TPK2

cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL203W / TPK2

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:-3.34|P-value:3.43E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.56|P-value:1.47E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:5.91|P-value:9.45E-10 ID:SGTC_258|Compound:haloperidol|FD-Score:3.18|P-value:6.10E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.05|P-value:9.75E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-4.45|P-value:3.08E-6 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.84|P-value:4.78E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:3.70E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:3.18|P-value:6.14E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.09|P-value:8.48E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:-3.37|P-value:3.10E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:4.03|P-value:2.15E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:4.65|P-value:1.13E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:6.02|P-value:4.71E-10 ID:SGTC_755|Compound:1995-0008|FD-Score:5.32|P-value:3.20E-8 ID:SGTC_821|Compound:1114-0266|FD-Score:3.48|P-value:2.04E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:3.37|P-value:3.06E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.13|P-value:7.45E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.24|P-value:5.00E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.31|P-value:3.88E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.05|P-value:9.55E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.94|P-value:3.10E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:4.34|P-value:5.21E-6 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.20|P-value:5.69E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.17|P-value:6.34E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.43|P-value:2.50E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.36|P-value:3.18E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-4.37|P-value:4.54E-6 ID:SGTC_1706|Compound:st031321|FD-Score:3.11|P-value:7.91E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.87|P-value:4.12E-5 ID:SGTC_1736|Compound:st036774|FD-Score:4.06|P-value:1.84E-5 ID:SGTC_1790|Compound:st045711|FD-Score:-3.33|P-value:3.48E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-3.39|P-value:2.88E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:3.33|P-value:3.53E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.81|P-value:5.49E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.28|P-value:4.38E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.05|P-value:1.92E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.40|P-value:3.90E-6 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.61|P-value:1.23E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.73|P-value:7.53E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.93|P-value:3.24E-5 ID:SGTC_3054|Compound:9094397|FD-Score:4.16|P-value:1.16E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.17|P-value:6.39E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:-3.34|P-value:3.43E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.56|P-value:1.47E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:5.91|P-value:9.45E-10 ID:SGTC_258|Compound:haloperidol|FD-Score:3.18|P-value:6.10E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.05|P-value:9.75E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-4.45|P-value:3.08E-6 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.84|P-value:4.78E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:3.70E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:3.18|P-value:6.14E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.09|P-value:8.48E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:-3.37|P-value:3.10E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:4.03|P-value:2.15E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:4.65|P-value:1.13E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:6.02|P-value:4.71E-10 ID:SGTC_755|Compound:1995-0008|FD-Score:5.32|P-value:3.20E-8 ID:SGTC_821|Compound:1114-0266|FD-Score:3.48|P-value:2.04E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:3.37|P-value:3.06E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.13|P-value:7.45E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.24|P-value:5.00E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.31|P-value:3.88E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.05|P-value:9.55E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.94|P-value:3.10E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:4.34|P-value:5.21E-6 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.20|P-value:5.69E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.17|P-value:6.34E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.43|P-value:2.50E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.36|P-value:3.18E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-4.37|P-value:4.54E-6 ID:SGTC_1706|Compound:st031321|FD-Score:3.11|P-value:7.91E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.87|P-value:4.12E-5 ID:SGTC_1736|Compound:st036774|FD-Score:4.06|P-value:1.84E-5 ID:SGTC_1790|Compound:st045711|FD-Score:-3.33|P-value:3.48E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-3.39|P-value:2.88E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:3.33|P-value:3.53E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.81|P-value:5.49E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.28|P-value:4.38E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.05|P-value:1.92E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.40|P-value:3.90E-6 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.61|P-value:1.23E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.73|P-value:7.53E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.93|P-value:3.24E-5 ID:SGTC_3054|Compound:9094397|FD-Score:4.16|P-value:1.16E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.17|P-value:6.39E-4

Top fitness defect scores for YPL203W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_637 1181-0519 6.02 7.3 µM 4.71E-10 fatty acid desaturase (OLE1) 53 8.67E-10
2 SGTC_176 1486-1328 5.91 72.8 µM 9.45E-10 22 1.70E-9
3 SGTC_755 1995-0008 5.32 37.3 µM 3.20E-8 8 5.16E-8
4 SGTC_634 1611-4317 4.65 11.2 µM 1.13E-6 ERG2 9 1.64E-6
5 SGTC_2584 androsterone 4.40 48.7 µM 3.90E-6 cell wall 98 5.43E-6
6 SGTC_1307 1187-1599 4.34 12.2 µM 5.21E-6 PDR1 8 7.19E-6
7 SGTC_3054 9094397 4.16 49.5 µM 1.16E-5 fatty acid desaturase (OLE1) 26 1.56E-5
8 SGTC_1736 st036774 4.06 51.0 µM 1.84E-5 30 2.45E-5
9 SGTC_2410 aloe-emodin-8-o-glycoside 4.05 46.3 µM 1.92E-5 mitochondrial processes 27 2.54E-5
10 SGTC_619 1652-1634 4.03 108.0 µM 2.15E-5 PDR1 20 2.85E-5
11 SGTC_1257 0669-0121 3.94 16.0 µM 3.10E-5 29 4.05E-5
12 SGTC_2959 9079155 3.93 11.0 µM 3.24E-5 26 4.23E-5
13 SGTC_453 enantio-paf c16 3.90 10.0 µM 3.70E-5 147 4.81E-5
14 SGTC_1707 st032323 3.87 6.3 µM 4.12E-5 DNA damage response 59 5.33E-5
15 SGTC_451 chloral hydrate 3.84 100.0 µM 4.78E-5 59 6.17E-5
16 SGTC_1969 st069479 3.81 40.1 µM 5.49E-5 15 7.05E-5
17 SGTC_2860 9025725 3.73 39.0 µM 7.53E-5 RPP1 & pyrimidine depletion 16 9.58E-5
18 SGTC_2750 bromebric acid 3.61 70.2 µM 1.23E-4 43 1.54E-4
19 SGTC_58 0957-0393 3.56 9.0 µM 1.47E-4 cell wall 421 1.83E-4
20 SGTC_821 1114-0266 3.48 29.2 µM 2.04E-4 34 2.52E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.09E-21 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138 7.96E-16 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.122 1.40E-12 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.111 1.20E-10 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.109 2.43E-10 YBR270C_p BIT2_p hom Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication
0.108 3.67E-10 YGL185C_p YGL185C_p hom Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.105 1.21E-9 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.104 1.39E-9 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.101 5.30E-9 YKL028W TFA1 het TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
0.100 5.93E-9 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.100 6.39E-9 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.099 8.43E-9 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.099 9.62E-9 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
0.097 1.85E-8 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
0.097 1.95E-8 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids