HIPHOP chemogenomics database

YPL208W / RKM1

SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL208W / RKM1

Click on Significant Values for Screen Details

Top fitness defect scores for YPL208W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2003	4023581	8.08	200.0 µM	9.95E-17	cell wall signaling	7	3.15E-16
2	SGTC_800	3448-7362	6.36	155.0 µM	5.12E-11	translation	36	1.04E-10
3	SGTC_2945	9067681	6.33	61.8 µM	5.92E-11		3	1.20E-10
4	SGTC_1728	st035614	5.95	33.8 µM	7.35E-10		8	1.37E-9
5	SGTC_791	1348-1292	5.03	225.0 µM	1.56E-7		17	2.43E-7
6	SGTC_786	4544-0069	4.97	423.0 µM	2.22E-7		11	3.41E-7
7	SGTC_978	1416-0512	4.83	87.4 µM	4.45E-7	redox potentiating	17	6.70E-7
8	SGTC_2434	5670830	4.69	169.9 µM	9.20E-7		12	1.35E-6
9	SGTC_2768	sulfasalazine	4.51	50.2 µM	2.26E-6		8	3.23E-6
10	SGTC_2942	9052777	4.31	62.2 µM	5.94E-6		28	8.21E-6
11	SGTC_2775	cyproheptadine	4.18	69.6 µM	1.07E-5		14	1.45E-5
12	SGTC_3211	9128781	4.08	49.5 µM	1.69E-5		53	2.26E-5
13	SGTC_882	0843-0131	4.04	351.0 µM	2.03E-5		16	2.69E-5
14	SGTC_864	0469-0573	3.98	172.0 µM	2.61E-5		20	3.44E-5
15	SGTC_2432	5630864	3.82	12.2 µM	5.14E-5		23	6.62E-5
16	SGTC_1677	st019204	3.72	53.9 µM	7.96E-5		26	1.01E-4
17	SGTC_2481	5478948	3.70	45.2 µM	8.66E-5	PDR1	43	1.10E-4
18	SGTC_66	0109-0187	3.64	42.4 µM	1.06E-4		47	1.34E-4
19	SGTC_1192	1488-0095	3.63	21.4 µM	1.11E-4		24	1.40E-4
20	SGTC_3272	9137413	3.51	49.5 µM	1.81E-4		24	2.24E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.253	2.49E-50	YOL100W	PKH2	hom	Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.220	4.84E-38	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.204	5.38E-33	YGL251C	HFM1	hom	Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.195	5.51E-30	YBR162C	TOS1	hom	Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.194	1.05E-29	YGL250W	RMR1	hom	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.185	3.15E-27	YIL039W	TED1	hom	Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.165	5.89E-22	YGL236C	MTO1	hom	Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.144	5.44E-17	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.139	5.59E-16	YNL067W	RPL9B	hom	Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.138	1.26E-15	YGR159C	NSR1	hom	Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.115	2.36E-11	YKL096C-B_p	YKL096C-B_p	hom	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.115	2.87E-11	YDR281C	PHM6	hom	Protein of unknown function, expression is regulated by phosphate levels
0.113	6.38E-11	YDR443C	SSN2	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.110	1.52E-10	YLL045C	RPL8B	hom	Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
0.110	1.55E-10	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin