YPL228W / CET1

Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL228W / CET1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.78|P-value:9.08E-5 ID:SGTC_13|Compound:0199-0087|FD-Score:5.35|P-value:5.76E-8 ID:SGTC_281|Compound:1672-3440|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.12|P-value:9.94E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.75|P-value:1.01E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.20|P-value:1.58E-5 ID:SGTC_906|Compound:1165-0455|FD-Score:3.35|P-value:4.44E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.41|P-value:3.83E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.46|P-value:3.04E-4 ID:SGTC_1687|Compound:st020778|FD-Score:-3.72|P-value:1.18E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.73|P-value:1.08E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.79|P-value:8.72E-5 ID:SGTC_2352|Compound:9027995|FD-Score:4.26|P-value:1.22E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.14|P-value:9.57E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.18|P-value:8.32E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.45|P-value:3.07E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.18|P-value:8.52E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.78|P-value:9.08E-5 ID:SGTC_13|Compound:0199-0087|FD-Score:5.35|P-value:5.76E-8 ID:SGTC_281|Compound:1672-3440|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.12|P-value:9.94E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.75|P-value:1.01E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.20|P-value:1.58E-5 ID:SGTC_906|Compound:1165-0455|FD-Score:3.35|P-value:4.44E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.41|P-value:3.83E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.46|P-value:3.04E-4 ID:SGTC_1687|Compound:st020778|FD-Score:-3.72|P-value:1.18E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.73|P-value:1.08E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.79|P-value:8.72E-5 ID:SGTC_2352|Compound:9027995|FD-Score:4.26|P-value:1.22E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.14|P-value:9.57E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.18|P-value:8.32E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.45|P-value:3.07E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.18|P-value:8.52E-4

Top fitness defect scores for YPL228W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_13 0199-0087 5.35 65.0 µM 5.76E-8 ERG2 1 4.42E-8 0.73
2 SGTC_2352 9027995 4.26 87.8 µM 1.22E-5 tubulin folding & SWR complex 2 1.03E-5 0.09
3 SGTC_820 1582-0060 4.20 59.9 µM 1.58E-5 10 1.35E-5 0.30
4 SGTC_1747 st037496 3.79 66.6 µM 8.72E-5 9 7.66E-5 0.06
5 SGTC_6 0844-0013 3.78 19.8 µM 9.08E-5 8 7.98E-5 0.03
6 SGTC_578 3460-0051 3.75 92.0 µM 1.01E-4 6 8.92E-5 0.26
7 SGTC_1731 st035964 3.73 63.0 µM 1.08E-4 15 9.54E-5 0.03
8 SGTC_1378 2093-0007 3.51 380.0 nM 2.53E-4 12 2.27E-4 0.19
9 SGTC_1473 k844-0725 3.46 134.0 µM 3.04E-4 10 2.73E-4 0.13
10 SGTC_3005 9076496 3.45 71.4 µM 3.07E-4 3 2.76E-4 0.36
11 SGTC_2962 9082311 3.43 13.2 µM 3.34E-4 18 3.01E-4 0.04
12 SGTC_1740 st038286 3.37 13.2 µM 4.16E-4 14 3.76E-4 0.04
13 SGTC_906 1165-0455 3.35 526.0 µM 4.44E-4 6 4.02E-4 0.05
14 SGTC_281 1672-3440 3.15 14.9 µM 8.99E-4 Golgi 4 8.23E-4 0.02
15 SGTC_572 0960-0087 3.12 17.7 µM 9.94E-4 21 9.12E-4 0.02
16 SGTC_1113 artemisinin 3.00 1.2 µM 0.00147 heme biosynthesis & mitochondrial translocase 44 0.00136 0.02
17 SGTC_1766 st045414 2.98 44.8 µM 0.00154 RPP1 & pyrimidine depletion 25 0.00143 0.07
18 SGTC_288 1315-0019 2.98 35.1 µM 0.00157 RSC & ERG11 35 0.00146 0.04
19 SGTC_2979 9012552 2.96 45.5 µM 0.00166 6 0.00153 0.04
20 SGTC_105 0111-0055 2.95 734.8 µM 0.00171 7 0.00159 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 3.63E-23 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.130 4.91E-14 YEL023C_p YEL023C_p hom Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
0.125 3.13E-13 YOR176W HEM15 het Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
0.118 8.28E-12 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.110 1.88E-10 YDL220C CDC13 het Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
0.108 3.82E-10 YOR180C DCI1 hom Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed
0.107 4.41E-10 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.101 5.02E-9 YLR319C BUD6 hom Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
0.099 1.14E-8 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.098 1.39E-8 YKL201C MNN4 hom Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.097 2.12E-8 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.094 4.27E-8 YGR096W TPC1 hom Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
0.093 6.98E-8 YLR366W_d YLR366W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
0.092 9.45E-8 YLR458W_d YLR458W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
0.092 1.02E-7 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress