YPL243W / SRP68

Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL243W / SRP68

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:7.75|P-value:9.98E-18 ID:SGTC_69|Compound:0096-0274|FD-Score:7.38|P-value:2.84E-16 ID:SGTC_86|Compound:0417-1630|FD-Score:2.84|P-value:9.38E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.07|P-value:4.06E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:2.83|P-value:9.79E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:2.87|P-value:8.30E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-3.41|P-value:8.53E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:3.46|P-value:7.65E-5 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-4.02|P-value:4.84E-6 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.21|P-value:2.02E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.55|P-value:3.15E-7 ID:SGTC_555|Compound:4296-0685|FD-Score:-2.83|P-value:9.05E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.01|P-value:4.88E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:2.88|P-value:8.28E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:2.85|P-value:9.20E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:3.18|P-value:2.48E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.11|P-value:3.01E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.04|P-value:4.41E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.11|P-value:3.28E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.42|P-value:1.38E-9 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.14|P-value:2.95E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.66|P-value:2.84E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.67|P-value:2.89E-5 ID:SGTC_1613|Compound:st002381|FD-Score:2.91|P-value:7.28E-4 ID:SGTC_1620|Compound:st003704|FD-Score:4.40|P-value:7.23E-7 ID:SGTC_1731|Compound:st035964|FD-Score:-2.90|P-value:7.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.08|P-value:3.43E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.34|P-value:1.15E-4 ID:SGTC_1962|Compound:st076595|FD-Score:2.98|P-value:5.63E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.97|P-value:5.42E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.30|P-value:1.52E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.29|P-value:1.56E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.78|P-value:1.77E-5 ID:SGTC_2430|Compound:5571206|FD-Score:2.89|P-value:7.91E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.04|P-value:4.32E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.16|P-value:2.73E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.48|P-value:9.39E-10 ID:SGTC_3231|Compound:9132313|FD-Score:3.32|P-value:1.38E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.62|P-value:3.68E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:7.75|P-value:9.98E-18 ID:SGTC_69|Compound:0096-0274|FD-Score:7.38|P-value:2.84E-16 ID:SGTC_86|Compound:0417-1630|FD-Score:2.84|P-value:9.38E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.07|P-value:4.06E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:2.83|P-value:9.79E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:2.87|P-value:8.30E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-3.41|P-value:8.53E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:3.46|P-value:7.65E-5 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-4.02|P-value:4.84E-6 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.21|P-value:2.02E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.55|P-value:3.15E-7 ID:SGTC_555|Compound:4296-0685|FD-Score:-2.83|P-value:9.05E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.01|P-value:4.88E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:2.88|P-value:8.28E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:2.85|P-value:9.20E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:3.18|P-value:2.48E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.11|P-value:3.01E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.04|P-value:4.41E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.11|P-value:3.28E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.42|P-value:1.38E-9 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.14|P-value:2.95E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.66|P-value:2.84E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.67|P-value:2.89E-5 ID:SGTC_1613|Compound:st002381|FD-Score:2.91|P-value:7.28E-4 ID:SGTC_1620|Compound:st003704|FD-Score:4.40|P-value:7.23E-7 ID:SGTC_1731|Compound:st035964|FD-Score:-2.90|P-value:7.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.08|P-value:3.43E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.34|P-value:1.15E-4 ID:SGTC_1962|Compound:st076595|FD-Score:2.98|P-value:5.63E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.97|P-value:5.42E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.30|P-value:1.52E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.29|P-value:1.56E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.78|P-value:1.77E-5 ID:SGTC_2430|Compound:5571206|FD-Score:2.89|P-value:7.91E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.04|P-value:4.32E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.16|P-value:2.73E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.48|P-value:9.39E-10 ID:SGTC_3231|Compound:9132313|FD-Score:3.32|P-value:1.38E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.62|P-value:3.68E-5

Top fitness defect scores for YPL243W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_16 erodoxin 7.75 22.8 µM 9.98E-18 unfolded protein response 5 4.76E-15 0.76
2 SGTC_69 0096-0274 7.38 206.7 µM 2.84E-16 mitochondrial processes 12 7.75E-14 0.27
3 SGTC_2645 abamectin 5.48 100.0 µM 9.39E-10 heme requiring 5 2.11E-8 0.19
4 SGTC_1161 k007-0610 5.42 110.0 µM 1.38E-9 ERAD & cell cycle 20 2.92E-8 0.19
5 SGTC_546 peri-Naphthalide 4.55 147.0 µM 3.15E-7 DNA damage response 18 2.73E-6 0.10
6 SGTC_1620 st003704 4.40 16.5 µM 7.23E-7 3 5.45E-6 0.49
7 SGTC_103 0844-0104 4.07 51.8 µM 4.06E-6 ERAD & cell cycle 48 2.32E-5 0.01
8 SGTC_2369 9071472 3.78 200.0 µM 1.77E-5 5 7.96E-5 0.26
9 SGTC_1585 2',4'-dihydroxychalcone 3.67 10.4 µM 2.89E-5 12 1.20E-4 0.04
10 SGTC_3311 9131146 3.62 2.9 µM 3.68E-5 cell wall 24 1.47E-4 0.07
11 SGTC_435 c-PAF 3.46 6.7 µM 7.65E-5 17 2.72E-4 0.06
12 SGTC_3231 9132313 3.32 49.5 µM 1.38E-4 4 4.46E-4 0.00
13 SGTC_2139 5578692 3.30 56.5 µM 1.52E-4 superoxide 25 4.85E-4 0.25
14 SGTC_2148 5546263 3.29 113.6 µM 1.56E-4 6 4.95E-4 0.02
15 SGTC_910 3454-3003 3.18 490.0 µM 2.48E-4 10 7.31E-4 0.00
16 SGTC_2598 perillyl alcohol 3.16 100.0 µM 2.73E-4 16 7.93E-4 0.01
17 SGTC_1298 1079-1278 3.14 263.0 µM 2.95E-4 DNA damage response 10 8.48E-4 0.01
18 SGTC_1157 4092-0394 3.11 27.6 µM 3.28E-4 10 9.26E-4 0.01
19 SGTC_2434 5670830 3.04 169.9 µM 4.32E-4 16 0.00117 0.05
20 SGTC_1103 duloxetine 3.04 36.1 µM 4.41E-4 16 0.00119 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.254 3.39E-50 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.244 1.15E-46 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.239 1.30E-44 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.224 1.67E-39 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.220 6.73E-38 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.196 2.11E-30 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.193 2.26E-29 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.176 1.12E-24 YPL210C SRP72 het Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
0.175 1.69E-24 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.175 1.93E-24 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.175 2.39E-24 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.153 6.64E-19 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.152 1.05E-18 YEL043W YEL043W hom Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
0.149 3.94E-18 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.147 1.16E-17 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication