YPL244C / HUT1

Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL244C / HUT1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:20.80|P-value:1.78E-95 ID:SGTC_24|Compound:0417-1663|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.25|P-value:5.68E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.50|P-value:2.31E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.93|P-value:4.16E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.55|P-value:2.25E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.52|P-value:2.14E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.19|P-value:6.85E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.60|P-value:1.57E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:6.95|P-value:2.00E-12 ID:SGTC_976|Compound:1348-1067|FD-Score:3.48|P-value:2.50E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.03|P-value:2.71E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.43|P-value:3.49E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.22|P-value:7.24E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.16|P-value:7.81E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.89|P-value:4.92E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.88|P-value:5.12E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.41|P-value:3.13E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.93|P-value:4.16E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.70|P-value:1.05E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.24|P-value:1.13E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.18|P-value:7.29E-4 ID:SGTC_1647|Compound:st011722|FD-Score:-3.77|P-value:9.63E-5 ID:SGTC_1699|Compound:st031318|FD-Score:-3.89|P-value:5.96E-5 ID:SGTC_1792|Compound:st045773|FD-Score:3.42|P-value:3.11E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.23|P-value:6.03E-4 ID:SGTC_1831|Compound:st055328|FD-Score:-3.62|P-value:1.69E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.34|P-value:4.80E-4 ID:SGTC_1934|Compound:st074720|FD-Score:4.32|P-value:7.67E-6 ID:SGTC_2421|Compound:5690543|FD-Score:3.42|P-value:3.11E-4 ID:SGTC_2477|Compound:5967686|FD-Score:6.56|P-value:2.91E-11 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.71|P-value:1.26E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.58|P-value:1.69E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.92|P-value:4.29E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.19|P-value:6.90E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.25|P-value:5.64E-4 ID:SGTC_3012|Compound:9080433|FD-Score:4.17|P-value:1.52E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:20.80|P-value:1.78E-95 ID:SGTC_24|Compound:0417-1663|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.25|P-value:5.68E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.50|P-value:2.31E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.93|P-value:4.16E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.55|P-value:2.25E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.52|P-value:2.14E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.19|P-value:6.85E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.60|P-value:1.57E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:6.95|P-value:2.00E-12 ID:SGTC_976|Compound:1348-1067|FD-Score:3.48|P-value:2.50E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.03|P-value:2.71E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.43|P-value:3.49E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.22|P-value:7.24E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.16|P-value:7.81E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.89|P-value:4.92E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.88|P-value:5.12E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.41|P-value:3.13E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.93|P-value:4.16E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.70|P-value:1.05E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.24|P-value:1.13E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.18|P-value:7.29E-4 ID:SGTC_1647|Compound:st011722|FD-Score:-3.77|P-value:9.63E-5 ID:SGTC_1699|Compound:st031318|FD-Score:-3.89|P-value:5.96E-5 ID:SGTC_1792|Compound:st045773|FD-Score:3.42|P-value:3.11E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.23|P-value:6.03E-4 ID:SGTC_1831|Compound:st055328|FD-Score:-3.62|P-value:1.69E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.34|P-value:4.80E-4 ID:SGTC_1934|Compound:st074720|FD-Score:4.32|P-value:7.67E-6 ID:SGTC_2421|Compound:5690543|FD-Score:3.42|P-value:3.11E-4 ID:SGTC_2477|Compound:5967686|FD-Score:6.56|P-value:2.91E-11 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.71|P-value:1.26E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.58|P-value:1.69E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.92|P-value:4.29E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.19|P-value:6.90E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.25|P-value:5.64E-4 ID:SGTC_3012|Compound:9080433|FD-Score:4.17|P-value:1.52E-5

Top fitness defect scores for YPL244C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_16 erodoxin 20.80 22.8 µM 1.78E-95 unfolded protein response 4 3.71E-96
2 SGTC_927 2425-1185 6.95 123.0 µM 2.00E-12 1 1.85E-12
3 SGTC_2477 5967686 6.56 25.2 µM 2.91E-11 4 2.73E-11
4 SGTC_2543 pimpinellin 4.71 60.7 µM 1.26E-6 Golgi 5 1.25E-6
5 SGTC_1934 st074720 4.32 32.6 µM 7.67E-6 38 7.71E-6
6 SGTC_1381 2324-0066 4.24 62.1 µM 1.13E-5 24 1.13E-5
7 SGTC_3012 9080433 4.17 71.4 µM 1.52E-5 8 1.53E-5
8 SGTC_1038 k292-0204 4.03 154.0 µM 2.71E-5 16 2.74E-5
9 SGTC_1239 0337-0584 3.93 35.4 µM 4.16E-5 unfolded protein response 55 4.21E-5
10 SGTC_623 k072-0108 3.93 16.9 µM 4.16E-5 heme biosynthesis & mitochondrial translocase 21 4.21E-5
11 SGTC_2646 retinol 3.92 96.8 µM 4.29E-5 9 4.34E-5
12 SGTC_1196 1588-0294 3.89 184.0 µM 4.92E-5 15 4.98E-5
13 SGTC_1200 1643-0193 3.88 18.4 µM 5.12E-5 19 5.19E-5
14 SGTC_1380 2291-0730 3.70 164.0 µM 1.05E-4 32 1.07E-4
15 SGTC_24 0417-1663 3.65 219.0 µM 1.27E-4 24 1.29E-4
16 SGTC_817 1391-0715 3.60 46.0 µM 1.57E-4 25 1.60E-4
17 SGTC_2625 euphol 3.58 100.0 µM 1.69E-4 38 1.72E-4
18 SGTC_785 1270-0111 3.52 65.3 µM 2.14E-4 47 2.18E-4
19 SGTC_199 4063-0024 3.50 41.6 µM 2.31E-4 10 2.35E-4
20 SGTC_976 1348-1067 3.48 47.6 µM 2.50E-4 60S ribosome export 49 2.55E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.485 2.19E-197 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.430 4.67E-151 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.313 2.54E-77 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.301 4.68E-71 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.245 7.02E-47 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.244 1.15E-46 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.210 9.19E-35 YCL051W LRE1 hom Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
0.207 8.70E-34 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.207 9.87E-34 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.204 8.94E-33 YCL052C PBN1 het Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
0.192 4.14E-29 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.187 1.02E-27 YPR111W DBF20 hom Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs
0.174 3.13E-24 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.173 5.86E-24 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.156 8.80E-20 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast