HIPHOP chemogenomics database

YPL262W / FUM1

Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL262W / FUM1

Click on Significant Values for Screen Details

Top fitness defect scores for YPL262W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_184	1494-0393	17.00	24.7 µM	2.81E-61		1	1.96E-65
2	SGTC_219	mitoxanthrone	14.50	164.4 µM	4.82E-45	DNA intercalators	3	5.14E-48
3	SGTC_996	3970-0799	9.23	1.8 µM	1.86E-19		16	1.35E-20
4	SGTC_36	0335-0881	5.69	14.2 µM	1.57E-8	ubiquinone biosynthesis & proteasome	70	6.33E-9
5	SGTC_3283	9109332	5.04	35.8 µM	4.65E-7	DNA intercalators	4	2.33E-7
6	SGTC_100	0086-0128	4.85	49.6 µM	1.14E-6	copper-dependent oxidative stress	25	6.04E-7
7	SGTC_1269	0831-0796	4.59	3.0 µM	3.85E-6	60S ribosome export	7	2.21E-6
8	SGTC_1557	sumaresinolic acid	4.55	42.3 µM	4.66E-6		8	2.70E-6
9	SGTC_2858	9025781	4.33	9.0 µM	1.23E-5	sphingolipid biosynthesis & PDR1	81	7.57E-6
10	SGTC_2279	7948423	4.33	32.9 µM	1.23E-5		7	7.57E-6
11	SGTC_145	3737-0113	4.19	38.4 µM	2.21E-5	DNA intercalators	68	1.41E-5
12	SGTC_90	0870-0863	4.00	4.6 µM	4.77E-5		45	3.19E-5
13	SGTC_86	0417-1630	3.89	471.9 µM	7.35E-5	ubiquinone biosynthesis & proteasome	156	5.06E-5
14	SGTC_1353	1498-1206	3.79	8.1 µM	1.08E-4	mitochondrial response to ROS	58	7.63E-5
15	SGTC_1926	methyl fluorone black	3.77	53.6 µM	1.16E-4	DNA intercalators	69	8.16E-5
16	SGTC_1551	6-bromo-3'-methylflavone	3.75	63.5 µM	1.23E-4		24	8.72E-5
17	SGTC_892	0929-0076	3.74	29.0 µM	1.29E-4	TRP & mitochondrial translation	50	9.14E-5
18	SGTC_3000	9075062	3.73	71.4 µM	1.35E-4		25	9.66E-5
19	SGTC_628	1636-0253	3.65	16.0 µM	1.80E-4	sphingolipid biosynthesis & PDR1	43	1.30E-4
20	SGTC_3319	9138450	3.65	16.8 µM	1.82E-4		29	1.32E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.241	2.21E-45	YMR044W	IOC4	hom	Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
0.219	1.52E-37	YML118W	NGL3	hom	3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.219	1.38E-37	YPL035C_d	YPL035C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.208	3.67E-34	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.190	1.68E-28	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.184	6.03E-27	YJR116W_p	TDA4_p	hom	Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
0.178	3.14E-25	YGR035C_p	YGR035C_p	hom	Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.177	6.68E-25	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.166	5.08E-22	YGR070W	ROM1	hom	GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.165	6.64E-22	YMR166C_p	YMR166C_p	hom	Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.165	6.60E-22	YDL099W	BUG1	hom	Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.165	7.55E-22	YDR406W	PDR15	hom	Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.165	1.04E-21	YBR123C	TFC1	het	One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
0.160	1.72E-20	YPL197C_d	YPL197C_d	hom	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.157	7.37E-20	YGL222C	EDC1	hom	RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress