HIPHOP chemogenomics database

YPL273W / SAM4

S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL273W / SAM4

Click on Significant Values for Screen Details

Top fitness defect scores for YPL273W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2045	5106652	5.43	78.0 µM	4.72E-9		2	2.88E-8
2	SGTC_1291	0993-0025	5.36	5.8 µM	7.13E-9		2	4.17E-8
3	SGTC_3075	9116269	5.17	49.5 µM	2.30E-8		6	1.19E-7
4	SGTC_360	0957-0399	4.96	1.0 µM	7.48E-8	cell wall	24	3.44E-7
5	SGTC_3013	9080133	4.67	71.4 µM	3.90E-7		7	1.51E-6
6	SGTC_2814	7977482	4.03	43.0 µM	9.94E-6		49	2.77E-5
7	SGTC_2059	5238311	4.02	157.0 µM	1.06E-5		24	2.95E-5
8	SGTC_1247	0448-0043	3.91	5.8 µM	1.75E-5	ERG2	44	4.60E-5
9	SGTC_3222	9128301	3.86	49.5 µM	2.18E-5	60S ribosome export	27	5.62E-5
10	SGTC_3180	9109287	3.84	49.5 µM	2.42E-5		15	6.17E-5
11	SGTC_3329	9141233	3.81	27.6 µM	2.72E-5		17	6.84E-5
12	SGTC_777	1315-0167	3.67	517.0 µM	5.23E-5	Golgi	35	1.23E-4
13	SGTC_1055	3448-0672	3.61	86.0 µM	6.62E-5		41	1.52E-4
14	SGTC_2318	6029776	3.57	164.0 µM	8.06E-5		43	1.82E-4
15	SGTC_1143	3454-2408	3.51	5.1 µM	1.02E-4	60S ribosome export	5	2.24E-4
16	SGTC_268	camptothecin	3.36	39.6 µM	1.86E-4	DNA damage response	110	3.86E-4
17	SGTC_1836	st055642	3.30	81.0 µM	2.36E-4		45	4.78E-4
18	SGTC_1599	8-chlro-1-tetrahydronorharmanone	3.30	90.6 µM	2.39E-4		57	4.84E-4
19	SGTC_270	tamoxifen	3.25	5.5 µM	2.95E-4	NEO1	66	5.84E-4
20	SGTC_3103	9120822	3.24	49.5 µM	2.99E-4		38	5.92E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.168	1.46E-22	YNL244C	SUI1	het	Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
0.120	2.98E-12	YLR097C	HRT3	hom	Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.111	1.29E-10	YOR060C	SLD7	het	Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.100	5.85E-9	YNL209W	SSB2	hom	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.098	1.24E-8	YNL104C	LEU4	hom	Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.094	4.29E-8	YDR020C_p	DAS2_p	hom	Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.093	6.15E-8	YLR259C	HSP60	het	Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
0.091	1.14E-7	YKR012C_d	YKR012C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2
0.089	2.56E-7	YOR103C	OST2	het	Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.088	3.06E-7	YIL032C_d	YIL032C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.088	3.77E-7	YDR217C	RAD9	hom	DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.081	2.37E-6	YDL086W_p	YDL086W_p	hom	Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
0.081	2.69E-6	YDL230W	PTP1	hom	Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
0.081	2.69E-6	YCR102W-A_d	YCR102W-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.081	2.86E-6	YJR150C	DAN1	hom	Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth